Construct: ORF ccsbBroad304_11952
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001965.1_s304c1, BRDN0000462167
- Derived from:
- ccsbBroadEn_11952
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KCTD3 (51133)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11952
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51133 | KCTD3 | potassium channel tetrameri... | NM_001319294.2 | 100% | 100% | |
| 2 | human | 51133 | KCTD3 | potassium channel tetrameri... | NM_016121.5 | 99.7% | 99.7% | 1747_1752delGATGTA |
| 3 | human | 51133 | KCTD3 | potassium channel tetrameri... | NM_001319295.2 | 87.4% | 87.2% | 0_1ins283;3_4insAAGCAGAA;6_7insTACGGGATCACTCCA |
| 4 | human | 51133 | KCTD3 | potassium channel tetrameri... | XM_005273158.2 | 87.2% | 86.9% | (many diffs) |
| 5 | mouse | 226823 | Kctd3 | potassium channel tetrameri... | XM_006497143.2 | 83.7% | 93.6% | (many diffs) |
| 6 | mouse | 226823 | Kctd3 | potassium channel tetrameri... | NM_172650.2 | 83.4% | 93.3% | (many diffs) |
| 7 | mouse | 226823 | Kctd3 | potassium channel tetrameri... | XM_011238911.2 | 75.3% | 82.7% | (many diffs) |
| 8 | mouse | 226823 | Kctd3 | potassium channel tetrameri... | XM_006497144.3 | 73.5% | 78.4% | (many diffs) |
| 9 | mouse | 226823 | Kctd3 | potassium channel tetrameri... | XM_017320173.1 | 55.2% | 61.7% | (many diffs) |
| 10 | mouse | 226823 | Kctd3 | potassium channel tetrameri... | XM_017320169.1 | 55.1% | 61.5% | (many diffs) |
| 11 | mouse | 226823 | Kctd3 | potassium channel tetrameri... | XM_017320182.1 | 47.4% | 53.5% | (many diffs) |
| 12 | mouse | 226823 | Kctd3 | potassium channel tetrameri... | XM_017320175.1 | 47.3% | 53.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2508
- ORF length:
- 2439
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcgggaggg cactgcggca gcttccccgc ggcggcggcc ggcagcggcg 121 agatcgtcca actgaacgta ggggggacca gatttagtac ctcaagacaa actcttatgt 181 ggattccaga ttcttttttt tccagtttgc tgagtgggag aatttcaaca cttcgagatg 241 aaactggtgc tatatttatt gatagagatc cagcagcatt tgcacccatt ttaaattttc 301 ttcggacaaa agaactagac ttaaggggag tgagtattaa tgttctcagg catgaagcag 361 aattttacgg gatcactcca ttagtaagaa ggcttctctt atgtgaagaa ttggagcgtt 421 cctcttgtgg cagtgtcctt tttcatggtt acttgccccc accaggtatt cctagtcgta 481 aaataaacaa cacagtcaga tctgctgatt ctaggaatgg tctaaattct acagaaggtg 541 aagcccgggg aaatggtaca cagcctgttc tctctggaac gggagaagaa actgttaggc 601 taggatttcc tgtggatcca cgaaaggtgc taatagtagc tggccatcac aactggattg 661 tagctgcata tgcccatttt gctgtgtgtt acagaatcaa agaatcttca ggatggcagc 721 aagtgtttac gagcccatat ttggattgga ctatcgaacg agtagcttta aatgcaaagg 781 tggttggagg gccacatgga gacaaagaca aaatggttgc tgttgcctca gagagtagca 841 tcatcttgtg gagtgttcag gatgggggaa gtggaagtga aattggagtg ttcagcctgg 901 gtgttcctgt agatgctctc ttctttattg gtaaccagtt ggtggccacg agtcatacag 961 ggaaagtggg agtgtggaat gctgtcactc agcactggca ggttcaagat gttgttccta 1021 taactagtta tgacactgct ggatcattcc ttctgcttgg atgtaacaat ggatcaatat 1081 attacataga tatgcagaag ttccccttgc gaatgaaaga taatgatctt cttgtaactg 1141 aactgtatca tgatccttca aatgatgcta ttactgctct gagtgtttac ctcacaccca 1201 aaacaagtgt cagtggtaac tggatcgaga tcgcctatgg tacgagctct ggagcagtac 1261 gagtgattgt acaacaccca gagacagttg ggtcaggtcc tcagcttttt cagactttca 1321 cagttcaccg aagtcccgta acaaaaatca tgctatcaga gaagcatctt gtatcagtct 1381 gtgcagataa taatcatgtc cggacgtgga cagtaacacg attcagagga atgatctcta 1441 ctcagccagg ttctactcct ttagcgtcat tcaagatact atccctggag gagacagaaa 1501 gtcatggtag ctattcctct ggaaatgaca taggaccttt tggagagcga gacgatcaac 1561 aggtgtttat ccagaaagtt gttcccatca ccaacaaact atttgtaaga ctctcatcga 1621 ctggaaaaag aatatgtgag atccaggctg ttgactgtac tacaatatcc tcatttacag 1681 tgagggaatg tgagggatcc agtaggatgg gctcaagacc aaggcgctac ttgttcacag 1741 gccatacaaa tggcagtatt caaatgtggg atctgaccac tgctatggat atggttaaca 1801 aaagtgaaga taagggtggt ccaaccgaag aagagctact caaattactc gatcaatgtg 1861 atttgagcac atctcgctgt gctactccta acatcagtcc agcaacttcc gtagttcagc 1921 atagccactt acgagaatca aattctagcc ttcagcttca gcaccatgat accacccatg 1981 aagcagctac ttacggttcc atgaggcctt acagagaaag tcctttatta gcaagggcaa 2041 gaaggactga gagctttcac agttataggg acttccagac tattaatttg aacagaaatg 2101 tagaaagagc tgtccctgaa aatggtaact tgggtccaat acaagctgaa gtgaaagggg 2161 caacagggga atgtaatata tctgagagaa agtctcctgg agtagaaata aaaagtttga 2221 gagaattgga tagtggattg gaagtgcata aaatagctga aggtttttca gaatccaaga 2281 aaaggtcatc agaagatgaa aatgaaaata aaatagagtt taggaagaaa ggaggatttg 2341 aagggggagg attccttgga agaaagaaag ttccctatct ggcgtcatca ccaagtactt 2401 ccgatggagg aactgactca cctggtactg cgtccccatc tcctacaaag actactccat 2461 ctcctcggca taaaaaaagt gattcttcag gtcaggagta cagcttgttg ccaactttct 2521 tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt 2581 aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta