Transcript: Human NM_001319295.2

Homo sapiens potassium channel tetramerization domain containing 3 (KCTD3), transcript variant 3, mRNA.

Source:
NCBI, updated 2019-08-07
Taxon:
Homo sapiens (human)
Gene:
KCTD3 (51133)
Length:
4085
CDS:
761..2896

Additional Resources:

NCBI RefSeq record:
NM_001319295.2
NBCI Gene record:
KCTD3 (51133)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001319295.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000044525 GCCACTTACGAGAATCAAATT pLKO.1 2310 CDS 100% 13.200 18.480 N KCTD3 n/a
2 TRCN0000044526 CGAGACGATCAACAGGTGTTT pLKO.1 1934 CDS 100% 4.950 6.930 N KCTD3 n/a
3 TRCN0000044524 GCCCATATTTGGATTGGACTA pLKO.1 1119 CDS 100% 4.050 5.670 N KCTD3 n/a
4 TRCN0000069415 CGAGATGAAACTGGTGCTATA pLKO.1 548 5UTR 100% 10.800 8.640 N Kctd3 n/a
5 TRCN0000044527 CCACGAAAGGTGCTAATAGTA pLKO.1 1004 CDS 100% 5.625 4.500 N KCTD3 n/a
6 TRCN0000421426 TAGGGACTTCCAGACTATTAA pLKO_005 2452 CDS 100% 15.000 10.500 N KCTD3 n/a
7 TRCN0000429792 GATGTAACAATGGATCAATAT pLKO_005 1446 CDS 100% 13.200 9.240 N KCTD3 n/a
8 TRCN0000044523 CCTCATTTACAGTGAGGGAAT pLKO.1 2055 CDS 100% 0.405 0.284 N KCTD3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001319295.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11952 pDONR223 100% 87.4% 87.2% None 0_1ins283;3_4insAAGCAGAA;6_7insTACGGGATCACTCCA n/a
2 ccsbBroad304_11952 pLX_304 0% 87.4% 87.2% V5 0_1ins283;3_4insAAGCAGAA;6_7insTACGGGATCACTCCA n/a
3 TRCN0000477450 AATATCTAGTGGAAGTGATTGTGA pLX_317 11.8% 87.4% 87.2% V5 0_1ins283;3_4insAAGCAGAA;6_7insTACGGGATCACTCCA n/a
4 ccsbBroadEn_08229 pDONR223 100% 87.1% 86.9% None (many diffs) n/a
5 ccsbBroad304_08229 pLX_304 0% 87.1% 86.9% V5 (many diffs) n/a
6 TRCN0000492353 GAATGTATTCGATCCTGATCAGAA pLX_317 20.6% 87.1% 86.9% V5 (many diffs) n/a
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