Construct: ORF ccsbBroad304_11961
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001963.1_s304c1, BRDN0000410846
- Derived from:
- ccsbBroadEn_11961
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HSPA14 (51182)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11961
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51182 | HSPA14 | heat shock protein family A... | NM_001278205.1 | 56.2% | 50.3% | (many diffs) |
| 2 | human | 51182 | HSPA14 | heat shock protein family A... | NM_016299.4 | 16.6% | 14.6% | (many diffs) |
| 3 | human | 51182 | HSPA14 | heat shock protein family A... | NR_103464.2 | 5.6% | 1_97del;362_4660del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 330
- ORF length:
- 264
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc ggccatcgga gttcacctgg gctgcacctc agcctgtgtg gccgtctata 121 aggatggccg ggctggtgtg gttgcaaatg atgccggtga ccgagttact ccagctgttg 181 ttgcttactc agaaaatgaa gagattgttg gattggcagc aaaacaaagt agaataagaa 241 atatttcaaa tacagtaatg aaagtaaagc agatcctggg cagaagccag aaatgcggtc 301 cttggacctg gcttctcagc aattatccct gcccaacttt cttgtacaaa gtggttggta 361 agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc 421 ggtggcgcgt taagtcgaca atcaacctct ggatta