Construct: ORF ccsbBroad304_11968

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF015649.1_s304c1, BRDN0000404901
Derived from:
ccsbBroadEn_11968
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
NT5C3A (51251)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_11968
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 51251 NT5C3A 5'-nucleotidase, cytosolic ... NM_001002010.4 100% 100%
2 human 51251 NT5C3A 5'-nucleotidase, cytosolic ... NM_001374335.1 90% 90% 137_138ins99
3 human 51251 NT5C3A 5'-nucleotidase, cytosolic ... NM_001002009.3 86.4% 86.4% (many diffs)
4 human 51251 NT5C3A 5'-nucleotidase, cytosolic ... NM_016489.13 86.4% 86.4% (many diffs)
5 human 51251 NT5C3A 5'-nucleotidase, cytosolic ... NM_001166118.3 86.1% 86.1% 0_1ins138
6 human 51251 NT5C3A 5'-nucleotidase, cytosolic ... NM_001356996.2 86.1% 86.1% 0_1ins138
7 human 51251 NT5C3A 5'-nucleotidase, cytosolic ... NM_001374336.1 86.1% 86.1% 0_1ins138
8 human 51251 NT5C3A 5'-nucleotidase, cytosolic ... NM_001374337.1 86.1% 86.1% 0_1ins138
9 human 51251 NT5C3A 5'-nucleotidase, cytosolic ... NM_001374338.1 79.7% 79.7% 693_694ins201
10 human 51251 NT5C3A 5'-nucleotidase, cytosolic ... NM_001374339.1 77.6% 77.3% (many diffs)
11 mouse 107569 Nt5c3 5'-nucleotidase, cytosolic III NM_026004.3 86.6% 93.6% (many diffs)
12 mouse 107569 Nt5c3 5'-nucleotidase, cytosolic III XM_006505274.2 75.9% 80.6% (many diffs)
13 mouse 107569 Nt5c3 5'-nucleotidase, cytosolic III XM_006505275.2 74.5% 80.3% (many diffs)
14 mouse 107569 Nt5c3 5'-nucleotidase, cytosolic III NM_001252374.1 74.3% 80.4% (many diffs)
15 mouse 107569 Nt5c3 5'-nucleotidase, cytosolic III NM_001360963.1 74.3% 80.4% (many diffs)
16 mouse 107569 Nt5c3 5'-nucleotidase, cytosolic III XM_006505273.2 74.3% 80.4% (many diffs)
17 mouse 107569 Nt5c3 5'-nucleotidase, cytosolic III XM_011241111.2 74.3% 80.4% (many diffs)
18 mouse 107569 Nt5c3 5'-nucleotidase, cytosolic III XM_030255051.1 74.3% 80.4% (many diffs)
19 mouse 107569 Nt5c3 5'-nucleotidase, cytosolic III XM_006505276.4 74.2% 80% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1059
ORF length:
993
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgga ccgcgcggcc gtggcgaggg tgggcgcggt agcgagcgcc agcgtgtgcg
121ccctggtggc gggggtggtg ctggctcagt acatattcac cttgaagagg aagacggggc
181ggaagaccaa gatcatcgag atgatgccag aattccagaa aagttcagtt cgaatcaaga
241accctacaag agtagaagaa attatctgtg gtcttatcaa aggaggagct gccaaacttc
301agataataac ggactttgat atgacactca gtagattttc atataaaggg aaaagatgcc
361caacatgtca taatatcatt gacaactgta agctggttac agatgaatgt agaaaaaagt
421tattgcaact aaaggaaaaa tattacgcta ttgaagttga tcctgttctt actgtagaag
481agaagtaccc ttatatggtg gaatggtata ctaaatcaca tggtttgctt gttcagcaag
541ctttaccaaa agctaaactt aaagaaattg tggcagaatc tgacgttatg ctcaaagaag
601gatatgagaa tttctttgat aagctccaac aacatagcat ccccgtgttc atattttcgg
661ctggaatcgg cgatgtacta gaggaagtta ttcgtcaagc tggtgtttat catcccaatg
721tcaaagttgt gtccaatttt atggattttg atgaaactgg ggtgctcaaa ggatttaaag
781gagaactaat tcatgtattt aacaaacatg atggtgcctt gaggaataca gaatatttca
841atcaactaaa agacaatagt aacataattc ttctgggaga ctcccaagga gacttaagaa
901tggcagatgg agtggccaat gttgagcaca ttctgaaaat tggatatcta aatgatagag
961tggatgagct tttagaaaag tacatggact cttatgatat tgttttagta caagatgaat
1021cattagaagt agccaactct attttacaga agattctata cccaactttc ttgtacaaag
1081tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta
1141gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta

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