Construct: ORF ccsbBroad304_12068

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016782.1_s304c1, BRDN0000404336
Derived from:
ccsbBroadEn_12068
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MINDY2 (54629)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_12068
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 54629 MINDY2 MINDY lysine 48 deubiquitin... XM_017022335.2 88.3% 86.3% (many diffs)
2 human 54629 MINDY2 MINDY lysine 48 deubiquitin... XM_017022334.2 66.8% 64.2% (many diffs)
3 human 54629 MINDY2 MINDY lysine 48 deubiquitin... XM_017022333.2 66.7% 64% (many diffs)
4 human 54629 MINDY2 MINDY lysine 48 deubiquitin... XM_017022332.2 64.6% 62% (many diffs)
5 human 54629 MINDY2 MINDY lysine 48 deubiquitin... XM_024449964.1 64.3% 64.5% 1122_1123insGGTG;1134_1758del
6 human 54629 MINDY2 MINDY lysine 48 deubiquitin... XM_011521690.2 64.1% 64.3% 1122_1123insGGTG;1134_1761del
7 human 54629 MINDY2 MINDY lysine 48 deubiquitin... XM_011521689.2 62.2% 62.4% 964_1017del;1176_1177insGGTG;1188_1815del
8 human 54629 MINDY2 MINDY lysine 48 deubiquitin... NM_001040453.3 60.7% 60.9% 1021_1122del;1224_1225insGGTG;1236_1860del
9 human 54629 MINDY2 MINDY lysine 48 deubiquitin... NM_001040450.3 60.6% 60.8% 1021_1122del;1224_1225insGGTG;1236_1863del
10 human 54629 MINDY2 MINDY lysine 48 deubiquitin... XR_001751324.2 59.2% (many diffs)
11 human 54629 MINDY2 MINDY lysine 48 deubiquitin... XM_011521688.2 59% 59.2% (many diffs)
12 human 54629 MINDY2 MINDY lysine 48 deubiquitin... XM_011521687.2 58.9% 59.1% (many diffs)
13 human 54629 MINDY2 MINDY lysine 48 deubiquitin... XR_001751323.2 58.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1203
ORF length:
1137
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgga gagcagcccc gagagcctgc agccgctaga acacggggtg gcggccgggc
121cagcgtcagg gacaggttct tcgcaggaag ggctacagga gaccaggctc gccgctggtg
181atggtcctgg ggtatgggcg gcggagacca gcggcgggaa tgggctgggg gcggcggccg
241ccaggaggag cctcccggac tcggcttctc ccgcgggctc tcctgaggtt cccggaccct
301gcagctcctc cgcgggtttg gacttgaagg acagtggttt ggagagtcct gctgccgccg
361aggcgcctct gagagggcag tacaaggtga ccgcctcccc ggagacagcc gtggccggag
421tgggtcatga gttgggtacc gccggagacg cgggagcccg cccggatctc gccggcacct
481gccaagcaga actgaccgcc gccggctccg aagagcccag cagcgccggc ggcctcagca
541gcagttgcag cgacccgagc cctcctgggg aatctccgag cctggactct ctggagtcgt
601tctctaacct gcattctttt cccagtagct gcgagttcaa tagtgaggag ggagcggaga
661acagggtccc tgaggaggag gagggcgcgg cggtgttgcc cggggctgtt cctctgtgca
721aggaggagga gggggaggag accgctcagg tgctggcggc ctccaaggaa cgcttcccgg
781gacaatctgt gtatcacatc aagtggatcc agtggaagga agagaacaca cccatcatca
841cccagaatga gaacggaccc tgccccttgc tggccatcct caatgttttg ctcctggcct
901ggaaggtgaa acttccaccg atgatggaaa tcataactgc tgagcagctg atggaatatt
961taggagatta catgcttgat gcaaagccaa aagaaatttc agaaattcaa cgtttaaatt
1021atgaacagaa tatgagtgat gccatggcaa ttttgcacaa actacagaca ggcctggatg
1081taaatattga tgacattgta aaagctgttg gtaactgcag ctacaaccaa ctagtggaga
1141agatcatctc ttgtaaacag tcagacaata gtgagctggt tagtgaaggt gggctttgta
1201gctgcccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc
1261tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc
1321tctggatta

Download FASTA (ORF) (Full)

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