Transcript: Human XM_011521690.2

PREDICTED: Homo sapiens MINDY lysine 48 deubiquitinase 2 (MINDY2), transcript variant X4, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
MINDY2 (54629)
Length:
9192
CDS:
139..1902

Additional Resources:

NCBI RefSeq record:
XM_011521690.2
NBCI Gene record:
MINDY2 (54629)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011521690.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000144588 GAGATTACATGCTTGATGCAA pLKO.1 1037 CDS 100% 3.000 4.200 N MINDY2 n/a
2 TRCN0000140448 GAGTCGTTCTCTAACCTGCAT pLKO.1 667 CDS 100% 2.640 3.696 N MINDY2 n/a
3 TRCN0000142964 CCACCGATGATGGAAATCATA pLKO.1 988 CDS 100% 0.563 0.788 N MINDY2 n/a
4 TRCN0000283390 TGTGTGTTCTTTCGGAATAAT pLKO_005 1357 CDS 100% 15.000 10.500 N Mindy2 n/a
5 TRCN0000142831 CAGACAATAGTGAGCTGGTTA pLKO.1 1235 CDS 100% 4.950 3.465 N MINDY2 n/a
6 TRCN0000139925 GATCCAGTGGAAGGAAGAGAA pLKO.1 879 CDS 100% 4.950 3.465 N MINDY2 n/a
7 TRCN0000142735 CATGCTTGATGCAAAGCCAAA pLKO.1 1044 CDS 100% 4.050 2.835 N MINDY2 n/a
8 TRCN0000139982 GAAGGAAGAGAACACACCCAT pLKO.1 888 CDS 100% 2.640 1.848 N MINDY2 n/a
9 TRCN0000143316 GCAAAGAAACTCCAAGAGGAA pLKO.1 1672 CDS 100% 2.640 1.848 N MINDY2 n/a
10 TRCN0000145035 GCTTCTCAATACTATCAGGAA pLKO.1 1705 CDS 100% 2.640 1.848 N MINDY2 n/a
11 TRCN0000139924 GCTACAACCAACTAGTGGAGA pLKO.1 1193 CDS 100% 0.000 0.000 N MINDY2 n/a
12 TRCN0000166201 CATGGTGAAACCCTGTCTCTA pLKO.1 3028 3UTR 100% 4.950 2.475 Y ORAI2 n/a
13 TRCN0000078113 GCCTGTAATCCCAGCACTTTA pLKO.1 7601 3UTR 100% 13.200 6.600 Y LIAS n/a
14 TRCN0000256748 GGCAGGAGAATTGCTTGAATC pLKO_005 3115 3UTR 100% 10.800 5.400 Y SMIM11A n/a
15 TRCN0000179120 CAACATGGTGAAACCCTGTTT pLKO.1 7675 3UTR 100% 4.950 2.475 Y LOC339059 n/a
16 TRCN0000138772 GCAGGAGAATCGCTTGAACTT pLKO.1 4730 3UTR 100% 4.950 2.475 Y DCAF11 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011521690.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12068 pDONR223 100% 64.1% 64.3% None 1122_1123insGGTG;1134_1761del n/a
2 ccsbBroad304_12068 pLX_304 0% 64.1% 64.3% V5 1122_1123insGGTG;1134_1761del n/a
3 TRCN0000474250 TATTTAGCACGCGCTCTCCGTACT pLX_317 35% 64.1% 64.3% V5 1122_1123insGGTG;1134_1761del n/a
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