Construct: ORF ccsbBroad304_12078
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015277.1_s304c1, BRDN0000410680
- Derived from:
- ccsbBroadEn_12078
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RNF111 (54778)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12078
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54778 | RNF111 | ring finger protein 111 | NM_001270529.2 | 99.9% | 99.8% | 27C>G;444A>G |
| 2 | human | 54778 | RNF111 | ring finger protein 111 | XM_017022343.1 | 99.9% | 99.8% | 27C>G;444A>G |
| 3 | human | 54778 | RNF111 | ring finger protein 111 | NM_017610.8 | 99.8% | 99.7% | 27C>G;444A>G;1684_1686delCAG |
| 4 | human | 54778 | RNF111 | ring finger protein 111 | XM_017022342.1 | 99.8% | 99.7% | 27C>G;444A>G;1684_1686delCAG |
| 5 | human | 54778 | RNF111 | ring finger protein 111 | XM_017022340.2 | 99.1% | 99% | 27C>G;444A>G;2735_2758del |
| 6 | human | 54778 | RNF111 | ring finger protein 111 | XM_017022341.1 | 99.1% | 99% | 27C>G;444A>G;2735_2758del |
| 7 | human | 54778 | RNF111 | ring finger protein 111 | NM_001270530.1 | 99% | 98.9% | (many diffs) |
| 8 | human | 54778 | RNF111 | ring finger protein 111 | XM_017022338.2 | 99% | 98.9% | (many diffs) |
| 9 | human | 54778 | RNF111 | ring finger protein 111 | XM_017022339.2 | 99% | 98.9% | 27C>G;444A>G;2295_2321del |
| 10 | human | 54778 | RNF111 | ring finger protein 111 | NM_001270528.2 | 98.9% | 98.8% | (many diffs) |
| 11 | human | 54778 | RNF111 | ring finger protein 111 | XM_017022337.2 | 98.2% | 98.2% | (many diffs) |
| 12 | human | 54778 | RNF111 | ring finger protein 111 | NM_001330331.2 | 98.1% | 98.1% | (many diffs) |
| 13 | human | 54778 | RNF111 | ring finger protein 111 | XM_006720579.3 | 98.1% | 98.1% | (many diffs) |
| 14 | human | 54778 | RNF111 | ring finger protein 111 | XM_017022336.1 | 97.5% | 97.5% | (many diffs) |
| 15 | human | 54778 | RNF111 | ring finger protein 111 | XM_011521699.2 | 97% | 97% | (many diffs) |
| 16 | human | 54778 | RNF111 | ring finger protein 111 | XM_006720575.3 | 95.9% | 95.9% | (many diffs) |
| 17 | human | 54778 | RNF111 | ring finger protein 111 | XM_011521697.3 | 95.5% | 95.5% | (many diffs) |
| 18 | human | 54778 | RNF111 | ring finger protein 111 | XM_011521696.2 | 95.4% | 95.4% | (many diffs) |
| 19 | human | 54778 | RNF111 | ring finger protein 111 | XM_011521694.2 | 94.7% | 94.7% | (many diffs) |
| 20 | mouse | 93836 | Rnf111 | ring finger 111 | XM_006511587.3 | 88.3% | 90.3% | (many diffs) |
| 21 | mouse | 93836 | Rnf111 | ring finger 111 | XM_006511586.2 | 88.2% | 90.2% | (many diffs) |
| 22 | mouse | 93836 | Rnf111 | ring finger 111 | NM_033604.2 | 87.5% | 89.5% | (many diffs) |
| 23 | mouse | 93836 | Rnf111 | ring finger 111 | XM_006511582.2 | 86.7% | 88.7% | (many diffs) |
| 24 | mouse | 93836 | Rnf111 | ring finger 111 | XM_006511583.3 | 86.7% | 88.7% | (many diffs) |
| 25 | mouse | 93836 | Rnf111 | ring finger 111 | XM_006511584.2 | 86.7% | 88.7% | (many diffs) |
| 26 | mouse | 93836 | Rnf111 | ring finger 111 | XM_006511581.3 | 80.2% | 82.1% | (many diffs) |
| 27 | mouse | 93836 | Rnf111 | ring finger 111 | XM_006511580.3 | 80.1% | 82% | (many diffs) |
| 28 | mouse | 93836 | Rnf111 | ring finger 111 | XM_017313709.1 | 79.6% | 81.5% | (many diffs) |
| 29 | mouse | 93836 | Rnf111 | ring finger 111 | XM_006511578.3 | 79.6% | 81.4% | (many diffs) |
| 30 | mouse | 93836 | Rnf111 | ring finger 111 | XM_006511577.3 | 79.5% | 81.3% | (many diffs) |
| 31 | mouse | 93836 | Rnf111 | ring finger 111 | XM_011242841.2 | 79% | 80.8% | (many diffs) |
| 32 | mouse | 93836 | Rnf111 | ring finger 111 | XM_006511576.2 | 78.9% | 80.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3021
- ORF length:
- 2955
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtc tcaatggact cctgaatata aggagctcta caccttaaaa gtggatatga 121 agagtgagat tccttctgat gcaccaaaga cacaggagag tctgaaaggg atccttttgc 181 atccagagcc cattggggca gccaaaagtt ttcctgcagg agttgagatg attaatagta 241 aagtggggaa tgaattctct cacctgtgtg atgattctca aaagcaagag aaggaaatga 301 atggtaacca gcaagaacaa gaaaaaagtc tcgttgtgag gaaaaaacgc aaaagccagc 361 aggctggccc ttcgtatgtg cagaattgtg ttaaagaaaa ccagggaata ttaggactga 421 ggcaacacct agggacacca agtgatgaag ataatgattc ctcttttagt gattgtcttt 481 cttctccttc atctagtctg cattttgggg attctgatac tgtgacttca gatgaggata 541 aagaagtctc tgtaagacat tcccagacca ttttgaatgc taaaagtaga agccatagtg 601 cacggtctca taagtggcct cggactgaga cagaatctgt atcgggattg ttaatgaaaa 661 gaccctgttt acatggcagt tcgttacgga gacttccatg cagaaagaga tttgtaaaaa 721 ataattcctc acagaggaca cagaaacaaa aagagaggat attaatgcag aggaagaaac 781 gagaagtgtt agctcgaaga aaatatgcct tgctacctag ttctagtagt tccagtgaga 841 atgacctcag cagtgaatcc tcttctagct catcaactga aggagaagaa gatttgtttg 901 tttctgccag tgaaaaccac caaaacaatc cagctgttcc ctcaggaagt attgatgaag 961 atgttgtggt gatagaagct tcctccactc cccaggttac tgccaatgaa gaaattaatg 1021 ttacctcaac tgacagtgaa gtggagattg taacagttgg agaaagctat cggtctcgtt 1081 caacccttgg acactccaga tctcattgga gccagggttc cagttctcat gcaagtcggc 1141 cacaggagcc acggaaccgc agtaggattt ctactgttat acagcccttg aggcagaatg 1201 cagcagaagt tgtggacctt accgttgatg aagatgaacc tactgtagta ccaaccactt 1261 ctgcaagaat ggaatcacaa gctactagcg cttccattaa caattcaaat ccatctacct 1321 ctgagcaggc ctctgatact gcttcagctg tcaccagtag ccaaccttcc acagtgtcag 1381 agacttcagc tactcttaca agcaatagta ccactggcac ttctatagga gatgactcaa 1441 ggagaactac atctagtgct gtaacggaaa ctggccctcc tgcaatgcca aggttacctt 1501 cctgctgtcc ccagcactca ccatgtggag ggtcgtcaca gaaccaccat gcattaggac 1561 atcctcatac aagttgcttt cagcagcatg gtcaccattt tcaacatcat caccaccacc 1621 accatactcc ccacccagct gtcccagttt ctccttcctt tagtgatcct gcttgccctg 1681 tggaaagacc tccacaagta caagcacctt gtggagcaaa tagtagttct ggtaccagct 1741 atcatgaaca ggcattgcca gtggacctga gcaacagtgg tatcagaagt catggaagtg 1801 gcagttttca tggagcatct gcatttgacc cctgctgccc tgtttcttcc tcccgagctg 1861 caatctttgg ccatcaggcc gctgctgctg ccccaagtca acctttatca tcaatagatg 1921 gctatggatc aagcatggtt gcgcagcccc agccccagcc ccctccacag ccctctctct 1981 catcatgtcg acattacatg ccaccccctt atgcctcttt gacaaggcca cttcatcatc 2041 aagcttctgc ctgcccgcat tctcatggaa acccccctcc tcagactcag cctccgcctc 2101 aagtggatta tgttattcct catcctgtac atgctttcca ttctcaaata tcttctcatg 2161 caacatctca tcctgtggca cccccaccac caactcactt agccagtaca gctgcaccaa 2221 tccctcagca tcttcctcct acacaccagc caatttcgca ccatattcca gccacagcac 2281 ctccagcaca gagactgcat cctcatgaag tgatgcagag gatggaagtt caaaggagga 2341 ggatgatgca gcatccaacg cgggcacatg aacgcccccc accccatcca cataggatgc 2401 acccaaacta tggtcatggg catcatattc atgtgcctca gactatgtcc tcacatcctc 2461 gacaggctcc agagaggtct gcctgggaac tgggaattga agctggagtg actgcagcta 2521 cttatacacc tggtgcattg catcctcact tggcccatta tcacgcacct cctcgacttc 2581 atcacttaca attaggagct cttcctttaa tggttcctga tatggcaggc tatcctcaca 2641 tccgttacat ttcatcagga ttggatggaa catcattcag aggtcctttc aggggcaatt 2701 ttgaggaact gattcatttg gaagaaagat taggcaatgt caatcgtgga gcatcccagg 2761 ggacaattga aagatgtaca tatccacata aatacaaaaa gaggaaactg cactgcaaac 2821 aagatgggga agaagggact gaggaagaca cagaggaaaa atgtactatc tgtttgtcta 2881 ttttagagga aggtgaagat gtgagacgtc ttccatgtat gcaccttttc caccaagtgt 2941 gtgttgacca atggttgatt accaataaga agtgccccat atgcagagtg gacattgagg 3001 cccagctgcc aagtgaaagt tgcccaactt tcttgtacaa agtggttggt aagcctatcc 3061 ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg 3121 ttaagtcgac aatcaacctc tggatta