Construct: ORF ccsbBroad304_12119
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000405.1_s304c1, BRDN0000402096
- Derived from:
- ccsbBroadEn_12119
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TRNAU1AP (54952)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12119
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54952 | TRNAU1AP | tRNA selenocysteine 1 assoc... | NM_017846.5 | 61.6% | 61.6% | 1_330del |
| 2 | human | 54952 | TRNAU1AP | tRNA selenocysteine 1 assoc... | NR_003109.1 | 26.1% | 1_572del;1104_2032del | |
| 3 | mouse | 71787 | Trnau1ap | tRNA selenocysteine 1 assoc... | XM_011250332.2 | 91.7% | 98.3% | (many diffs) |
| 4 | mouse | 71787 | Trnau1ap | tRNA selenocysteine 1 assoc... | XM_011250334.1 | 91.7% | 98.3% | (many diffs) |
| 5 | mouse | 71787 | Trnau1ap | tRNA selenocysteine 1 assoc... | XM_017320388.1 | 91.7% | 98.3% | (many diffs) |
| 6 | mouse | 71787 | Trnau1ap | tRNA selenocysteine 1 assoc... | XM_006539180.3 | 73.4% | 78.7% | (many diffs) |
| 7 | mouse | 71787 | Trnau1ap | tRNA selenocysteine 1 assoc... | NM_027925.3 | 56.5% | 60.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 597
- ORF length:
- 531
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgct gtatgaattc ttcgtcaaag tctacccctc ctgtcgggga ggcaaggtgg 121 ttttggacca gacaggcgtg tctaagggtt atggttttgt gaaattcaca gatgaactgg 181 aacagaagcg agccctgacg gagtgccagg gagcagtggg actggggtct aagcctgtgc 241 ggctgagcgt ggcaatccct aaagcgagcc gtgtaaagcc agtggaatat agtcagatgt 301 acagttatag ctacaaccag tattatcagc agtaccagaa ctactatgct cagtggggct 361 atgaccagaa cacaggcagc tacagctaca gttaccccca gtatggctat acccagagca 421 ccatgcagac atatgaagaa gttggagatg atgcattgga agaccccatg ccacagctgg 481 atgtgactga ggccaacaag gagttcatgg aacagagtga ggagctgtat gacgctctga 541 tggactgtca ctggcagccc ctggacacag tgtcttcaga gatccctgcc atgatgtacc 601 caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt 661 ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga 721 tta