Construct: ORF ccsbBroad304_12171

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008808.1_s304c1, BRDN0000407772
Derived from:
ccsbBroadEn_12171
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
RFWD3 (55159)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_12171
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55159 RFWD3 ring finger and WD repeat d... NM_001370534.1 96.6% 96.5% 1_75del;1175G>A;2309T>C
2 human 55159 RFWD3 ring finger and WD repeat d... NM_001370535.1 96.6% 96.5% 1_75del;1175G>A;2309T>C
3 human 55159 RFWD3 ring finger and WD repeat d... NM_018124.4 96.6% 96.5% 1_75del;1175G>A;2309T>C
4 human 55159 RFWD3 ring finger and WD repeat d... XM_005256022.4 96.6% 96.5% 1_75del;1175G>A;2309T>C
5 human 55159 RFWD3 ring finger and WD repeat d... XM_011523191.3 96.6% 96.5% 1_75del;1175G>A;2309T>C
6 human 55159 RFWD3 ring finger and WD repeat d... XM_017023391.1 96.6% 96.5% 1_75del;1175G>A;2309T>C
7 human 55159 RFWD3 ring finger and WD repeat d... NM_001370536.1 89% 88.8% (many diffs)
8 human 55159 RFWD3 ring finger and WD repeat d... XM_006721228.3 89% 88.8% (many diffs)
9 human 55159 RFWD3 ring finger and WD repeat d... NM_001370537.1 66.1% 65.9% 0_1ins759;341G>A;1475T>C
10 human 55159 RFWD3 ring finger and WD repeat d... NM_001370539.1 66.1% 65.9% 0_1ins759;341G>A;1475T>C
11 human 55159 RFWD3 ring finger and WD repeat d... NM_001370540.1 66.1% 65.9% 0_1ins759;341G>A;1475T>C
12 human 55159 RFWD3 ring finger and WD repeat d... NM_001370542.1 66.1% 65.9% 0_1ins759;341G>A;1475T>C
13 human 55159 RFWD3 ring finger and WD repeat d... NM_001370543.1 66.1% 65.9% 0_1ins759;341G>A;1475T>C
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2313
ORF length:
2247
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc cagcagccaa gggggaccag ccctcctcca gcctgttcct gctgatgtgg
121tcagcagcca gggggtacca tccatcctcc agccagctcc tgctgaggtg atcagcagcc
181aagcgacacc acccctgctc cagcctgctc cgcaactgtc tgttgacctg acagaagtgg
241aggtcttggg agaagacact gtggagaaca tcaatccaag aacttcagaa caacataggc
301agggatctga tggtaatcac accatcccag catcttcgtt gcattcaatg accaacttca
361tcagcggact gcagagactt catggcatgc tggaattcct gagaccttca tcttcaaacc
421acagtgtagg gccaatgaga acaagaagga gggtatctgc ttcacggagg gcaagagccg
481gagggtctca gaggacagac agtgccaggt tgagagcacc attggatgct tactttcagg
541tgagcaggac ccagcctgac ttgccagcta ccacttatga ttcagagact aggaatcctg
601tatctgaaga gttgcaggtg tctagtagtt ctgattctga cagtgacagc tctgcagagt
661atggaggggt tgttgaccag gcagaggaat ctggagctgt cattttagaa gagcaactag
721caggtgtctc agcagagcaa gaagttacat gtatcgatgg aggcaagacc ctccccaaac
781agccatctcc ccagaagtct gagcctctgc taccttctgc ttctatggat gaggaagaag
841gggacacttg tacaatatgt ctggaacagt ggaccaatgc tggggaccac cggctctcag
901cattacgctg tgggcatctc tttgggtata ggtgcatttc cacgtggctt aaaggacaag
961tacgaaaatg tccccagtgc aacaagaaag ccaggcacag tgacattgtc gtcctttatg
1021cccgaaccct gagagctttg gacactagtg aacaggagcg catgaaaagt tccctactga
1081aggaacagat gctaaggaaa caggccgagt tagaatcagc acagtgccga ctccaactgc
1141aggtcctcac tgataagtgc actaagcttc aaaggcgtgt tcaggacttg caaaaactta
1201cgtcacatca aagtcagaat ttacagcaac ccaggggctc ccaagcatgg gtcctgagct
1261gctcaccctc cagccagggc cagcacaagc acaagtacca cttccaaaag accttcacag
1321tatctcaggc aggaaactgc cggatcatgg catactgtga tgctctgagc tgcctggtga
1381tatcacagcc ttctcctcag gcctcttttc ttccaggctt tggtgttaag atgttgagta
1441ctgccaacat gaagagcagt cagtacattc cgatgcatgg caaacagatc cgtggactgg
1501cgtttagcag ttacctcaga ggcttgctac tctctgcttc cctagacaac actattaaac
1561tgaccagcct ggagacaaat accgtggtcc agacttataa tgctggacgt cctgtctgga
1621gctgttgctg gtgtcttgat gaggctaact acatctatgc tggactggcc aatggttcaa
1681ttctggtata tgacgtgcga aacacgagca gtcatgtgca ggagttagta gctcagaaag
1741ccagatgccc actggtctcc ctgtcataca tgcccagagc tgcctcagct gcatttccat
1801atggtggggt gctggctgga accttggagg atgcttcatt ctgggaacag aaaatggact
1861tttctcattg gcctcatgtg ctgcccttgg agccaggggg ctgcatagac tttcagacag
1921agaacagctc ccggcactgt cttgtgacct acaggcctga taaaaatcac accaccatac
1981gaagtgtgct gatggaaatg tcctaccgac tggatgacac tggaaatcca atctgctcct
2041gccagcctgt acatacattt tttggaggac ctacttgcaa actattgacc aaaaatgcca
2101ttttccaaag cccagagaat gatggcaaca tcctggtgtg tactggggat gaagcagcaa
2161attctgccct gctgtgggat gctgccagtg gctcgttgct ccaggaccta cagaccgatc
2221agcctgtgtt ggacatctgc ccatttgagg tgaaccgtaa cagctacttg gctaccttaa
2281cagagaagat ggtccacacc tataagtggg agtgcccaac tttcttgtac aaagtggttg
2341gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta
2401accggtggcg cgttaagtcg acaatcaacc tctggatta

Download FASTA (ORF) (Full)