Construct: ORF ccsbBroad304_12175

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF006944.1_s304c1, BRDN0000407131
Derived from:
ccsbBroadEn_12175
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
INTS10 (55174)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_12175
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55174 INTS10 integrator complex subunit 10 NM_001353515.1 100% 100%
2 human 55174 INTS10 integrator complex subunit 10 NM_001353514.1 95.8% 95.8% 1638_1715del
3 human 55174 INTS10 integrator complex subunit 10 NM_001353513.1 95.6% 95.6% 801_803delGCA;1641_1718del
4 human 55174 INTS10 integrator complex subunit 10 NM_001353512.1 89.7% 89.7% 1_204del
5 human 55174 INTS10 integrator complex subunit 10 NM_001353511.1 86.3% 86.3% 1_204del;1842_1919del
6 human 55174 INTS10 integrator complex subunit 10 NM_018142.4 84% 84% 1_339del
7 human 55174 INTS10 integrator complex subunit 10 NM_001353510.1 83.9% 83.9% 1_339del;1140_1142delGCA
8 human 55174 INTS10 integrator complex subunit 10 NM_001353506.1 81.1% 81.1% 1_339del;1977_2054del
9 human 55174 INTS10 integrator complex subunit 10 NM_001353505.1 81% 81% 1_339del;1140_1142delGCA;1980_2057del
10 human 55174 INTS10 integrator complex subunit 10 NM_001353508.1 80.9% 80.9% 1_339del;835_836insGAA;1974_2051del
11 human 55174 INTS10 integrator complex subunit 10 NM_001353507.1 80.8% 80.8% (many diffs)
12 human 55174 INTS10 integrator complex subunit 10 NM_001353521.1 80.5% 80.5% 0_1ins348
13 human 55174 INTS10 integrator complex subunit 10 NM_001353522.1 80.5% 80.5% 0_1ins348
14 human 55174 INTS10 integrator complex subunit 10 XM_017013606.2 78.4% 77.4% (many diffs)
15 human 55174 INTS10 integrator complex subunit 10 NM_001353509.1 78.2% 78.2% 1_339del;772_773ins63;1914_1991del
16 human 55174 INTS10 integrator complex subunit 10 NM_001353517.1 77.2% 77.2% 0_1ins348;1290_1367del
17 human 55174 INTS10 integrator complex subunit 10 NM_001353518.1 77.2% 77.2% 0_1ins348;1290_1367del
18 human 55174 INTS10 integrator complex subunit 10 NM_001353519.1 77.2% 77.2% 0_1ins348;1290_1367del
19 human 55174 INTS10 integrator complex subunit 10 NM_001353516.1 77% 77% 0_1ins348;453_455delGCA;1293_1370del
20 human 55174 INTS10 integrator complex subunit 10 XM_017013607.1 74.4% 72.9% (many diffs)
21 human 55174 INTS10 integrator complex subunit 10 NM_001353520.1 73.8% 73.8% 0_1ins348;85_86ins63;1227_1304del
22 human 55174 INTS10 integrator complex subunit 10 XR_001745551.2 70.2% (many diffs)
23 human 55174 INTS10 integrator complex subunit 10 XR_001745552.2 67.9% (many diffs)
24 human 55174 INTS10 integrator complex subunit 10 XR_002956637.1 67.4% 1_486del;2124_2241del;2396_2654del
25 human 55174 INTS10 integrator complex subunit 10 XR_001745550.2 66.9% (many diffs)
26 human 55174 INTS10 integrator complex subunit 10 NR_148456.1 61.1% 1_728del;2029_2157del;2649_2929del
27 human 55174 INTS10 integrator complex subunit 10 NR_148454.1 60.6% (many diffs)
28 human 55174 INTS10 integrator complex subunit 10 NR_148453.1 59.6% (many diffs)
29 human 55174 INTS10 integrator complex subunit 10 NR_148458.1 59.3% 1_728del;2366_2585del;2740_3020del
30 human 55174 INTS10 integrator complex subunit 10 NR_148455.1 57.8% 1_728del;1224_1225ins170;2350_2630del
31 human 55174 INTS10 integrator complex subunit 10 NR_148451.1 57.1% (many diffs)
32 human 55174 INTS10 integrator complex subunit 10 NR_148452.1 53.8% (many diffs)
33 human 55174 INTS10 integrator complex subunit 10 NR_148457.1 53.6% (many diffs)
34 mouse 70885 Ints10 integrator complex subunit 10 NM_027590.4 73.2% 79.8% (many diffs)
35 mouse 70885 Ints10 integrator complex subunit 10 XM_006509747.3 73.1% 79.7% (many diffs)
36 mouse 70885 Ints10 integrator complex subunit 10 NM_001293792.1 70.6% 77% (many diffs)
37 mouse 70885 Ints10 integrator complex subunit 10 NM_001293791.1 70.6% 76.9% (many diffs)
38 mouse 70885 Ints10 integrator complex subunit 10 XM_006509743.3 70.5% 76.9% (many diffs)
39 mouse 70885 Ints10 integrator complex subunit 10 XM_006509744.3 70.5% 76.7% (many diffs)
40 mouse 70885 Ints10 integrator complex subunit 10 XM_006509742.3 68.2% 74.2% (many diffs)
41 mouse 70885 Ints10 integrator complex subunit 10 XM_006509741.2 68.1% 74.1% (many diffs)
42 mouse 70885 Ints10 integrator complex subunit 10 XM_011242326.1 54.1% 57.8% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1857
ORF length:
1791
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgtt actaaaggtc acggaacaat gcttcaacac gttagaacga tcagaaatgt
121tgcttctact tttgaggcgc ttccctgaaa cggtggtgca gcatggggtt ggccttgggg
181aggcactatt agaggctgaa actattgaag aacaagaatc tccagtgaac tgctttagaa
241aattatttgt ttgtgatgtc cttcctctaa taattaacaa ccatgatgtt cgattacctg
301ccaatttatt gtataagtac ttgaacaaag cagctgaatt ttatatcaat tatgtcacta
361ggtctactca aatagaaaat cagcatcaag gcgcccagga tacatctgat ttaatgtcac
421ctagcaaacg tagctctcag aagtacataa tagaagggct gacggaaaaa tcatcccaga
481tcgtggaccc ttgggagagg ttgtttaaga ttttgaatgt tgttggaatg agatgtgaat
541ggcagatgga taaaggaaga cgaagctatg gagatatttt gcatagaatg aaggatctct
601gcagatacat gaacaacttt gatagtgaag cacatgcaaa atataaaaac caagtggtgt
661attccaccat gctggtcttc tttaagaatg cattccagta tgtcaacagc atacagccat
721ctctcttcca aggtcctaat gccccgagcc aagttccact ggttcttctt gaagatgtat
781cgaatgtgta tggtgatgta gaaattgatc gtaataaaca catccataaa aagaggaaac
841tagctgaagg aagagaaaaa accatgagtt cagacgatga agactgttcg gcgaaaggaa
901gaaatcgtca cattgtagtc aataaagccg aacttgctaa ctccactgaa gtgttagaaa
961gctttaaatt ggccagggag agctgggagt tgctctattc cctagaattc cttgacaaag
1021aatttacaag gatttgcttg gcctggaaga cggatacttg gctttggtta agaatcttcc
1081tcactgatat gatcatctat cagggtcaat ataaaaaggc gatagccagc ctgcatcact
1141tagcagctct ccagggatcc atttctcagc cacagatcac agggcagggg accctggagc
1201atcagagggc gctcatccag ctggcgacgt gccactttgc gctaggggag tacagaatga
1261catgtgaaaa agtccttgat ttgatgtgct acatggtact ccccattcaa gatggaggca
1321aatcccagga ggaaccctcg aaagtaaagc ccaaatttag aaaaggttcg gatctgaagc
1381tcctgccttg taccagcaag gctatcatgc catactgcct ccatttaatg ttagcctgtt
1441ttaagcttag agctttcaca gacaacagag acgacatggc attggggcat gtgattgtgt
1501tgcttcagca agagtggcca cggggcgaga atcttttcct gaaagctgtc aataaaattt
1561gccaacaagg aaatttccaa tatgagaatt ttttcaatta cgttacaaat attgatatgc
1621tggaggaatt tgcctacttg agaactcagg aaggtgggaa aattcatctg gaattactac
1681ccaatcaagg aatgctgatc aagcaccaca ctgtaactcg aggcatcacc aaaggcgtga
1741aggaggactt tcgcctggcc atggagcgcc aggtctcccg ctgtggagag aatctgatgg
1801tggttctgca caggttctgc attaatgaga agatcttgct ccttcagact ctgacctgcc
1861caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt
1921ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga
1981tta

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