Transcript: Mouse XM_006509747.3

PREDICTED: Mus musculus integrator complex subunit 10 (Ints10), transcript variant X5, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Ints10 (70885)
Length:
3438
CDS:
93..2228

Additional Resources:

NCBI RefSeq record:
XM_006509747.3
NBCI Gene record:
Ints10 (70885)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006509747.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000179947 CGAAGCTCACAACAACTATAA pLKO.1 992 CDS 100% 13.200 18.480 N Ints10 n/a
2 TRCN0000196148 GCCGACTTCAACATCCAGTAT pLKO.1 204 CDS 100% 4.950 6.930 N Ints10 n/a
3 TRCN0000127515 CGTAGCTCTCAGAAGTACATA pLKO.1 795 CDS 100% 5.625 4.500 N INTS10 n/a
4 TRCN0000182995 CCTCTAATAATCAACAACCAT pLKO.1 630 CDS 100% 3.000 2.400 N Ints10 n/a
5 TRCN0000179239 GCTGGAGGAATTTGCTTATTT pLKO.1 1988 CDS 100% 15.000 10.500 N Ints10 n/a
6 TRCN0000455083 TAACTCCCTTAGTTGCTTAAA pLKO_005 2309 3UTR 100% 13.200 9.240 N Ints10 n/a
7 TRCN0000180016 CCAAAGTAAAGCCCAAGTGTA pLKO.1 1708 CDS 100% 4.950 3.465 N Ints10 n/a
8 TRCN0000166364 CACACACACACACACACACAA pLKO.1 2970 3UTR 100% 4.950 2.475 Y KAAG1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006509747.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12175 pDONR223 100% 73.1% 79.7% None (many diffs) n/a
2 ccsbBroad304_12175 pLX_304 0% 73.1% 79.7% V5 (many diffs) n/a
3 TRCN0000481174 ATCGTGTCTAGATCAAACACAAGA pLX_317 27.3% 73.1% 79.7% V5 (many diffs) n/a
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