Construct: ORF ccsbBroad304_12176

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011992.1_s304c1, BRDN0000403117
Derived from:
ccsbBroadEn_12176
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SEC61A2 (55176)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_12176
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55176 SEC61A2 SEC61 translocon alpha 2 su... NM_001142627.2 95.1% 94.7% (many diffs)
2 human 55176 SEC61A2 SEC61 translocon alpha 2 su... NM_018144.4 87.5% 87.3% (many diffs)
3 human 55176 SEC61A2 SEC61 translocon alpha 2 su... NM_001142628.1 82.9% 82.7% (many diffs)
4 human 29927 SEC61A1 SEC61 translocon alpha 1 su... NM_013336.4 68.8% 81.5% (many diffs)
5 human 55176 SEC61A2 SEC61 translocon alpha 2 su... NR_024576.2 55.5% (many diffs)
6 human 55176 SEC61A2 SEC61 translocon alpha 2 su... NR_024577.2 52.4% (many diffs)
7 mouse 57743 Sec61a2 Sec61, alpha subunit 2 (S. ... NM_021305.3 79.2% 87.3% (many diffs)
8 mouse 57743 Sec61a2 Sec61, alpha subunit 2 (S. ... XM_017319150.1 50.1% 55% (many diffs)
9 mouse 57743 Sec61a2 Sec61, alpha subunit 2 (S. ... XR_373943.2 46.8% (many diffs)
10 mouse 57743 Sec61a2 Sec61, alpha subunit 2 (S. ... XM_006497517.3 39.7% 44.5% (many diffs)
11 mouse 57743 Sec61a2 Sec61, alpha subunit 2 (S. ... XM_017319151.1 39.7% 44.5% (many diffs)
12 mouse 57743 Sec61a2 Sec61, alpha subunit 2 (S. ... XM_017319152.1 39.7% 44.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1320
ORF length:
1254
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggg catcaaattt ttagaagtta tcaaaccatt ctgtgcagtt ctaccagaaa
121ttcagaaacc ggaaaggaaa atccagttta gagagaaggt tctgtggact gctataacgc
181tcttcatttt cttagtgtgt tgtcagatcc cactgtttgg aatcatgtca tcagattctg
241cagatccttt ctactggatg agagttattc tggcttccaa tagaggaact ttaatggaat
301tgggtatctc cccaattgta acatctggtt tgattatgca gttgttagct ggagccaaaa
361tcattgaagt tggagataca ccgaaagata gagctttatt caatggagcc cagaaactgt
421ttggtatgat cattaccatt gggcaagcca ttgtgtatgt catgacgggg atgtatgggg
481accctgcaga aatgggtgct ggaatctgtc tcctgatcat cattcagttg tttgttgctg
541gtttgattgt gctgctgtta gatgagctgc tacagaaggg ttacggcttg gggtctggga
601tttccctctt tattgccacc aacatctgtg agaccattgt ctggaaggcc tttagtccca
661ctaccattaa cactggcaga ggtactgagt ttgagggtgc agtcatagct ctgttccatt
721tgttggccac caggacggac aaagtccgag ctttacggga ggctttttat cggcagaact
781tacccaatct catgaacctc attgctacag tttttgtgtt tgctgttgtt atatatttcc
841aaggatttcg cgttgatctg cccattaagt cggcccgtta ccgaggacag tacagcagct
901accccatcaa actcttctac acctccaaca tccccatcat cctccagtcg gccctggtgt
961ccaacctgta tgttatttcc cagatgctgt ctgttcgatt tagtggcaac tttttagtaa
1021atttactagg acagtgggcc gatgtcagtg ggggaggacc cgcacgttct tacccagttg
1081gaggcctttg ttactatctt tctcctcctg agtccatggg cgccatcttt gaggatcctg
1141tccatgtcgt tgtttatatc atcttcatgt tggggtcatg tgcattcttc tctaagacat
1201ggattgaagt gtctggttcc tcagccaaag atgtagctaa acagctgaaa gaacagcaga
1261tggtaatgag gggccaccga gatacctcta tggttcatga gcttaatagc gctagagcgt
1321acccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg
1381attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct
1441ggatta

Download FASTA (ORF) (Full)