Construct: ORF ccsbBroad304_12210
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008570.1_s304c1, BRDN0000410340
- Derived from:
- ccsbBroadEn_12210
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HJURP (55355)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12210
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55355 | HJURP | Holliday junction recogniti... | XM_011511437.1 | 75.7% | 75.6% | 1_462del;548C>G |
| 2 | human | 55355 | HJURP | Holliday junction recogniti... | NM_001282963.1 | 72.6% | 72.5% | 1_543del;629C>G |
| 3 | human | 55355 | HJURP | Holliday junction recogniti... | NM_001282962.1 | 69.4% | 69.3% | 1_636del;722C>G |
| 4 | human | 55355 | HJURP | Holliday junction recogniti... | NM_018410.5 | 64.3% | 64.3% | 1_798del;884C>G |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1512
- ORF length:
- 1446
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgct gcactccatg agccggctgt tgagcacaaa gccatcaagc atcatctcca 121 ccaaaacgtt catcatgcaa aactggaact gcaggaggag gcacagatat aagagcagga 181 tgaacaaaac atattgcaaa ggagccagac gttctcagag gagctccaag gagaacttca 241 taccctgctc tgagcctgtg aaagggacag gggcattaag agattgcaag aacgtattag 301 atgtttcttg ccgtaagaca ggtttaaaat tggaaaaagc ttttcttgaa gtcaacagac 361 cccaaatcca taagttagat ccaagttgga aggagcgcaa agtgacaccc tcgaagtatt 421 cttccttgat ttacttcgac tccagtgcaa catataatct tgatgaggaa aatagattta 481 ggacattaaa atggttaatt tctcctgtaa aaatagtttc cagaccaaca atacgacagg 541 gccatggaga gaaccgtcag agggagattg aaatccgatt tgatcagctt catcgggaat 601 attgcctgag tcccaggaac cagcctcgcc ggatgtgcct cccggactcc tgggccatga 661 acatgtacag agggggtcct gcgagtcctg gtggccttca gggcttagaa acccgcaggc 721 tgagtttacc ttccagcaaa gcaaaagcaa aaagtttaag tgaggctttt gaaaacctag 781 gcaaaagatc tctggaagca ggtaggtgcc tgcccaagag cgattcatct tcatcacttc 841 caaagaccaa ccccacacac agcgcaactc gcccgcagca gacatctgac cttcacgttc 901 agggaaatag ttctggaata tttagaaagt cagtgtcacc cagcaaaact ctttcagtcc 961 cagataaaga agtgccaggc cacggaagga atcgttacga tgaaattaaa gaagaatttg 1021 acaagcttca tcaaaagtat tgcctcaaat ctcctgggca gatgacagtg cctttatgta 1081 ttggagtgtc tacagataaa gcaagtatgg aagttcgata tcaaacagaa ggcttcttag 1141 gaaaattaaa tccagaccct cacttccagg gtttccagaa gttgccatca tcacccctgg 1201 ggtgcagaaa aagtctactg ggctcaactg caattgaggc tccttcatct acatgtgttg 1261 ctcgtgccat cacgagggat ggcacgaggg accatcagtt ccctgcaaaa agacccaggc 1321 tatcagaacc ccagggctcc ggacgccagg gcaattccct gggtgcctca gatggggtgg 1381 acaacaccgt cagaccggga gaccagggca gctcttcaca gcccaactca gaagagagag 1441 gagagaacac gtcttacagg atggaagaga aaagtgattt catgctagaa aaattggaaa 1501 ctaaaagtgt gtacccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc 1561 tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga 1621 caatcaacct ctggatta