Construct: ORF ccsbBroad304_12233
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003150.1_s304c1, BRDN0000461341
- Derived from:
- ccsbBroadEn_12233
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FERMT1 (55612)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12233
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55612 | FERMT1 | fermitin family member 1 | NM_017671.4 | 61.9% | 61.7% | 1_771del;787C>A;863G>A |
| 2 | human | 55612 | FERMT1 | fermitin family member 1 | XM_024451935.1 | 61.9% | 61.7% | 1_771del;787C>A;863G>A |
| 3 | mouse | 241639 | Fermt1 | fermitin family member 1 | XM_006499507.1 | 62.4% | 64% | (many diffs) |
| 4 | mouse | 241639 | Fermt1 | fermitin family member 1 | NM_198029.2 | 53.6% | 55% | (many diffs) |
| 5 | mouse | 241639 | Fermt1 | fermitin family member 1 | XM_017318206.1 | 53.6% | 55% | (many diffs) |
| 6 | mouse | 241639 | Fermt1 | fermitin family member 1 | XM_017318207.1 | 53.6% | 55% | (many diffs) |
| 7 | mouse | 241639 | Fermt1 | fermitin family member 1 | XM_017318210.1 | 53.6% | 55% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1329
- ORF length:
- 1260
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaacaaggc atcaaagagg atgagcagct gctcttacga tttaaatatt 121 attctttctt cgacttgaat cctaaatatg atgctgtcca aataaaccaa ctctatgagc 181 aagccaggtg ggccattctc ttagaagaaa ttgattgcac agaggaagaa atgttgatct 241 ttgcagctct acagtaccac attagcaaac tgtcgttgtc tgctgaaaca caggattttg 301 caggcgagtc cgaggttgat gaaatagaag cggcgctttc taatttggaa gtaaccctag 361 aaggtggaaa agcggacagc cttttggagg acattactga tatccctaaa cttgcagata 421 atctcaaatt atttaggccc aagaagttac taccaaaagc tttcaaacaa tattggttta 481 tctttaaaga cacatccata gcatacttta aaaataagga acttgaacaa ggagaaccac 541 tagaaaaact aaatcttaga ggctgcgaag ttgtgcccga tgtaaatgta gcaggaagaa 601 aatttggaat caagttacta atccctgttg ccgatggtat gaatgaaatg tatttgagat 661 gtgaccatga gaatcaatac gcccaatgga tggctgcctg catgttggca tcgaagggca 721 aaaccatggc agacagctcc taccagccag aggtcctcaa catcctttca tttctgagga 781 tgaaaaacag gaactctgca tctcaggtgg cttccagtct cgaaaacatg gatatgaacc 841 cagaatgttt tgtgtcacca cggtgtgcaa aaagacacaa atccaaacag ctggccgccc 901 ggatcctgga ggcgcaccag aacgtggccc agatgcccct ggtcgaagcc aagctgcggt 961 tcatccaggc gtggcagtca ctgcctgagt ttggcctcac ctactacctt gtcagattta 1021 aaggaagcaa aaaagatgac attctgggag tttcatataa caggttgatt aaaattgatg 1081 cagccaccgg gattccagtg acaacatgga gattcacaaa tatcaaacag tggaatgtaa 1141 actgggaaac ccggcaggtg gtcatcgagt ttgaccaaaa cgtctttact gctttcacct 1201 gcctgagtgc agattgcaag attgtgcacg agtacattgg cggctacatt ttcttgtcca 1261 cccgctccaa ggaccagaat gaaacactcg atgaggactt gttccacaaa ttgaccggcg 1321 gtcaggattt gccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc 1381 tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa 1441 tcaacctctg gatta