Transcript: Human XM_024451935.1

PREDICTED: Homo sapiens fermitin family member 1 (FERMT1), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
FERMT1 (55612)
Length:
5219
CDS:
858..2891

Additional Resources:

NCBI RefSeq record:
XM_024451935.1
NBCI Gene record:
FERMT1 (55612)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_024451935.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000083946 GCTCAAGTTAGTAGAACAGAT pLKO.1 986 CDS 100% 4.950 6.930 N FERMT1 n/a
2 TRCN0000413079 GCAGCTGCTCTTACGATTTAA pLKO_005 1655 CDS 100% 15.000 10.500 N FERMT1 n/a
3 TRCN0000425309 ATAATAAGGAACCCATAATTG pLKO_005 1321 CDS 100% 13.200 9.240 N FERMT1 n/a
4 TRCN0000083944 CCTACTACCTTGTCAGATTTA pLKO.1 2560 CDS 100% 13.200 9.240 N FERMT1 n/a
5 TRCN0000083945 CGCTTTCTAATTTGGAAGTAA pLKO.1 1894 CDS 100% 5.625 3.938 N FERMT1 n/a
6 TRCN0000175332 CACCTACTACCTTGTCAGATT pLKO.1 2558 CDS 100% 4.950 3.465 N Fermt1 n/a
7 TRCN0000083947 CTACAGTACCACATTAGCAAA pLKO.1 1809 CDS 100% 4.950 3.465 N FERMT1 n/a
8 TRCN0000083943 GCTGAGAACATCTCTGCCTAA pLKO.1 4800 3UTR 100% 4.050 2.430 N FERMT1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_024451935.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12233 pDONR223 100% 61.9% 61.7% None 1_771del;787C>A;863G>A n/a
2 ccsbBroad304_12233 pLX_304 0% 61.9% 61.7% V5 1_771del;787C>A;863G>A n/a
3 TRCN0000476913 CAATAACAACTACGCATTTTGACC pLX_317 25.7% 61.9% 61.7% V5 1_771del;787C>A;863G>A n/a
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