Construct: ORF ccsbBroad304_12254
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008194.1_s304c1, BRDN0000409533
- Derived from:
- ccsbBroadEn_12254
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- VAC14 (55697)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12254
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55697 | VAC14 | VAC14 component of PIKFYVE ... | NM_001351157.2 | 95.6% | 95.6% | 1_72del |
| 2 | human | 55697 | VAC14 | VAC14 component of PIKFYVE ... | NM_018052.5 | 67% | 67% | 1_774del |
| 3 | human | 55697 | VAC14 | VAC14 component of PIKFYVE ... | XM_005256038.4 | 62.2% | 59.5% | (many diffs) |
| 4 | human | 55697 | VAC14 | VAC14 component of PIKFYVE ... | XM_011523223.3 | 48.3% | 39.7% | (many diffs) |
| 5 | human | 55697 | VAC14 | VAC14 component of PIKFYVE ... | XM_005256041.4 | 47.5% | 40.5% | (many diffs) |
| 6 | human | 55697 | VAC14 | VAC14 component of PIKFYVE ... | XM_011523224.3 | 46.9% | 38.4% | (many diffs) |
| 7 | human | 55697 | VAC14 | VAC14 component of PIKFYVE ... | XM_017023441.2 | 46.3% | 37% | (many diffs) |
| 8 | human | 55697 | VAC14 | VAC14 component of PIKFYVE ... | XM_011523225.3 | 41.6% | 38.4% | (many diffs) |
| 9 | human | 55697 | VAC14 | VAC14 component of PIKFYVE ... | XM_017023442.2 | 40.9% | 38.7% | (many diffs) |
| 10 | mouse | 234729 | Vac14 | Vac14 homolog (S. cerevisiae) | NM_146216.2 | 59.3% | 61.5% | (many diffs) |
| 11 | mouse | 234729 | Vac14 | Vac14 homolog (S. cerevisiae) | XM_006530918.2 | 47.4% | 49% | (many diffs) |
| 12 | mouse | 234729 | Vac14 | Vac14 homolog (S. cerevisiae) | XR_001778432.1 | 40.7% | (many diffs) | |
| 13 | mouse | 234729 | Vac14 | Vac14 homolog (S. cerevisiae) | XM_006530919.3 | 33.9% | 33.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1638
- ORF length:
- 1572
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc caacatcctg gtgatccact gccagacaac agatgacctc atccagctga 121 cagccatgtg ctggatgcgg gagttcatcc agctggcggg ccgcgtcatg ctgccttact 181 cctccgggat cctgactgct gtcttgccct gcttggccta cgatgaccgc aagaaaagca 241 tcaaagaagt ggccaacgtg tgcaaccaga gcctgatgaa gctggtcacc cccgaggacg 301 acgagctgga tgagctgaga cctgggcaga ggcaggcaga gcccacccct gacgatgccc 361 tgccaaagca ggagggcaca gccagtggag gtccagatgg ttcctgtgac tccagcttca 421 gtagcggcat cagtgtcttc actgcagcca gcactgaaag agccccagtg acccttcacc 481 tcgacgggat cgtgcaggtc ctaaactgcc acctcagtga cacggccatt gggatgatga 541 ccaggattgc agttctcaag tggctctacc acctctacat caaaactcct cggaagatgt 601 tccggcacac ggacagcctc tttcccatcc tactgcagac gttatcggat gaatcggatg 661 aggtgatcct gaaggacctg gaggtgctgg cagaaatcgc ttcctccccc gcaggccaga 721 cggatgaccc aggccccctc gatggccctg acctccaggc cagccactca gagctccagg 781 tgcccacccc tggcagagcc ggcctactga acacctctgg taccaaaggc ttagaatgtt 841 ctccttcaac tcccaccatg aattcttact tttataagtt catgatcaac cttctcaaga 901 gattcagcag cgaacggaag ctcctggagg tcagaggccc tttcatcatc aggcagctgt 961 gcctcctgct gaatgcggag aacatcttcc actcaatggc agacatcctg ctgcgggagg 1021 aggacctcaa gttcgcctcg accatggtcc acgccctcaa caccatcctg ctgacctcca 1081 cagagctctt ccagctaagg aaccagctga aggacctgaa gaccctggag agccagaacc 1141 tgttctgctg cctgtaccgc tcctggtgcc acaacccagt caccacggtg tccctctgct 1201 tcctcaccca gaactaccgg cacgcctatg acctcatcca gaagtttggg gacctggagg 1261 tcaccgtgga cttcctcgca gaggtggaca agctggtgca gctgattgag tgccccatct 1321 tcacatatct gcgcctgcag ctgctggacg tgaagaacaa cccctacctg atcaaggccc 1381 tctacggcct gctcatgctc ctgccgcaga gcagcgcctt ccagctgctc tcgcaccggc 1441 tccagtgcgt gcccaaccct gagctgctgc agaccgaaga cagtctaaag gcagccccca 1501 agtcccagaa agctgactcc cctagcatcg actacgcaga gctgctgcag cactttgaga 1561 aggtccagaa caagcacctg gaagtgcggc accagcggag cgggcgtggg gaccacctgg 1621 accggagggt tgtcctctgc ccaactttct tgtacaaagt ggttggtaag cctatcccta 1681 accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta 1741 agtcgacaat caacctctgg atta