Construct: ORF ccsbBroad304_12288

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013399.1_s304c1, BRDN0000400061
Derived from:
ccsbBroadEn_12288
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ACSS2 (55902)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_12288
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55902 ACSS2 acyl-CoA synthetase short c... NM_001242393.1 72.9% 72.9% 1_492del
2 human 55902 ACSS2 acyl-CoA synthetase short c... XM_006723826.1 70.2% 70.2% 1_492del;547_585del;1302_1331del
3 human 55902 ACSS2 acyl-CoA synthetase short c... XM_011528912.2 68.8% 68.8% 1_531del;586_624del;1341_1370del
4 human 55902 ACSS2 acyl-CoA synthetase short c... XM_011528911.1 65.5% 65.5% 1_627del;682_720del;1437_1466del
5 human 55902 ACSS2 acyl-CoA synthetase short c... XM_011528909.2 64.3% 64.3% 1_666del;721_759del;1476_1505del
6 human 55902 ACSS2 acyl-CoA synthetase short c... NM_018677.4 63% 63% 1_777del
7 human 55902 ACSS2 acyl-CoA synthetase short c... XM_005260456.2 62.1% 62.1% 1_777del;1548_1577del
8 human 55902 ACSS2 acyl-CoA synthetase short c... NM_001076552.2 61.9% 61.9% 1_777del;832_870del
9 human 55902 ACSS2 acyl-CoA synthetase short c... XM_011528908.1 61.9% 61.9% 1_816del
10 human 55902 ACSS2 acyl-CoA synthetase short c... XM_005260455.2 61% 61% 1_777del;832_870del;1587_1616del
11 human 55902 ACSS2 acyl-CoA synthetase short c... XM_011528907.1 61% 61% 1_816del;1587_1616del
12 human 55902 ACSS2 acyl-CoA synthetase short c... XM_011528906.1 60.7% 60.7% 1_816del;871_909del
13 human 55902 ACSS2 acyl-CoA synthetase short c... XM_011528905.1 59.9% 59.9% 1_816del;871_909del;1626_1655del
14 mouse 60525 Acss2 acyl-CoA synthetase short-c... XM_006499975.1 73.6% 76.6% (many diffs)
15 mouse 60525 Acss2 acyl-CoA synthetase short-c... XM_006499974.1 65.1% 67.8% (many diffs)
16 mouse 60525 Acss2 acyl-CoA synthetase short-c... NM_019811.3 57.5% 59.9% (many diffs)
17 mouse 60525 Acss2 acyl-CoA synthetase short-c... XM_006499973.1 53.2% 55.4% (many diffs)
18 mouse 60525 Acss2 acyl-CoA synthetase short-c... XM_017319156.1 46.5% 48.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1392
ORF length:
1326
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggg tgactccacc agccagtccc ccccaattaa gaggtcatgc ccagatgtgc
121agatctcatg gaaccaaggg attgacttgt ggtggcatga gctcatgcaa gaggcagggg
181atgagtgtga gcccgagtgg tgtgatgccg aggacccact cttcatcctg tacaccagtg
241gctccacagg caaacccaag ggtgtggttc acacagttgg gggctacatg ctctatgtag
301ccacaacctt caagtatgtg tttgacttcc atgcagagga tgtgttctgg tgcacggcag
361acattggttg gatcactggt cattcctacg tcacctatgg gccactggcc aatggtgcca
421ccagtgtttt gtttgagggg attcccacat atccggacgt gaaccgcctg tggagcattg
481tggacaaata caaggtgacc aagttctaca cagcacccac agccatccgt ctgctcatga
541agtttggaga tgagcctgtc accaagcata gccgggcatc cttgcaggtg ttaggcacag
601tgggtgaacc catcaaccct gaggcctggc tatggtacca ccgggtggta ggtgcccagc
661gctgccccat cgtggacacc ttctggcaaa cagagacagg tggccacatg ttgactcccc
721ttcctggtgc cacacccatg aaacccggtt ctgctacttt cccattcttt ggtgtagctc
781ctgcaatcct gaatgagtcc ggggaagagt tggaaggtga agctgaaggt tatctggtgt
841tcaagcagcc ctggccaggg atcatgcgca cagtctatgg gaaccacgaa cgctttgaga
901caacctactt taagaagttt cctggatact atgttacagg agatggctgc cagcgggacc
961aggatggcta ttactggatc actggcagga ttgatgacat gctcaatgta tctggacacc
1021tgctgagtac agcagaggtg gagtcagcac ttgtggaaca tgaggctgtt gcagaggcag
1081ctgtggtggg ccaccctcat cctgtgaagg gtgaatgcct ctactgcttt gtcaccttgt
1141gtgatggcca caccttcagc cccaagctca ccgaggagct caagaagcag attagagaaa
1201agattggccc cattgccaca ccagactaca tccagaatgc acctggcttg cctaaaaccc
1261gctcagggaa aatcatgagg cgagtgcttc ggaagattgc tcagaatgac catgacctcg
1321gggacatgtc tactgtggct gacccatctg tcatcagtca cctcttcagc caccgctgcc
1381tgaccatcca gtgcccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc
1441tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga
1501caatcaacct ctggatta

Download FASTA (ORF) (Full)