Construct: ORF ccsbBroad304_12297
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009070.1_s304c1, BRDN0000400465
- Derived from:
- ccsbBroadEn_12297
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KIAA1217 (56243)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12297
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56243 | KIAA1217 | KIAA1217 | NM_001282769.2 | 94% | 94% | 1_165del |
| 2 | human | 56243 | KIAA1217 | KIAA1217 | NM_001282770.2 | 87% | 87% | 1_165del;2461_2462ins198 |
| 3 | human | 56243 | KIAA1217 | KIAA1217 | NM_001321681.2 | 71.7% | 71.4% | (many diffs) |
| 4 | human | 56243 | KIAA1217 | KIAA1217 | XM_011519564.2 | 65.6% | 65.4% | (many diffs) |
| 5 | human | 56243 | KIAA1217 | KIAA1217 | NM_001282767.1 | 63.6% | 63.4% | (many diffs) |
| 6 | human | 56243 | KIAA1217 | KIAA1217 | XM_024448085.1 | 62.9% | 62.6% | (many diffs) |
| 7 | human | 56243 | KIAA1217 | KIAA1217 | NM_001282768.1 | 61.8% | 61.6% | (many diffs) |
| 8 | human | 56243 | KIAA1217 | KIAA1217 | NM_001098500.3 | 60.9% | 60.6% | (many diffs) |
| 9 | human | 56243 | KIAA1217 | KIAA1217 | XM_017016429.1 | 60.9% | 60.6% | (many diffs) |
| 10 | human | 56243 | KIAA1217 | KIAA1217 | XM_024448086.1 | 60.9% | 60.6% | (many diffs) |
| 11 | human | 56243 | KIAA1217 | KIAA1217 | XM_011519566.2 | 59% | 58.7% | (many diffs) |
| 12 | human | 56243 | KIAA1217 | KIAA1217 | XM_005252516.4 | 58.8% | 57.6% | (many diffs) |
| 13 | human | 56243 | KIAA1217 | KIAA1217 | XM_017016427.2 | 58.7% | 58.4% | (many diffs) |
| 14 | human | 56243 | KIAA1217 | KIAA1217 | XM_011519562.2 | 57.3% | 57% | (many diffs) |
| 15 | human | 56243 | KIAA1217 | KIAA1217 | XM_017016425.2 | 57.2% | 57% | (many diffs) |
| 16 | human | 56243 | KIAA1217 | KIAA1217 | XM_017016426.2 | 56.3% | 56% | (many diffs) |
| 17 | human | 56243 | KIAA1217 | KIAA1217 | XM_011519565.3 | 55.6% | 55.3% | (many diffs) |
| 18 | human | 56243 | KIAA1217 | KIAA1217 | XM_017016423.1 | 51.8% | 50.8% | (many diffs) |
| 19 | human | 56243 | KIAA1217 | KIAA1217 | XM_017016424.1 | 51.8% | 50.8% | (many diffs) |
| 20 | human | 56243 | KIAA1217 | KIAA1217 | XM_017016422.1 | 49% | 48% | (many diffs) |
| 21 | human | 56243 | KIAA1217 | KIAA1217 | XM_017016420.1 | 48% | 47.1% | (many diffs) |
| 22 | human | 56243 | KIAA1217 | KIAA1217 | XM_017016421.1 | 48% | 47.1% | (many diffs) |
| 23 | human | 56243 | KIAA1217 | KIAA1217 | XM_017016419.2 | 47.1% | 46.2% | (many diffs) |
| 24 | human | 56243 | KIAA1217 | KIAA1217 | XM_024448082.1 | 46.2% | 45.3% | (many diffs) |
| 25 | human | 56243 | KIAA1217 | KIAA1217 | XM_024448083.1 | 46.2% | 45.3% | (many diffs) |
| 26 | human | 56243 | KIAA1217 | KIAA1217 | XM_024448084.1 | 46.2% | 45.3% | (many diffs) |
| 27 | human | 56243 | KIAA1217 | KIAA1217 | XM_017016418.2 | 46.2% | 45.3% | (many diffs) |
| 28 | human | 56243 | KIAA1217 | KIAA1217 | XM_017016417.1 | 45.1% | 44.2% | (many diffs) |
| 29 | human | 56243 | KIAA1217 | KIAA1217 | NM_019590.5 | 44.3% | 43.4% | (many diffs) |
| 30 | human | 56243 | KIAA1217 | KIAA1217 | XM_011519559.2 | 44% | 43.2% | (many diffs) |
| 31 | human | 56243 | KIAA1217 | KIAA1217 | XM_017016416.1 | 43.7% | 42.8% | (many diffs) |
| 32 | human | 56243 | KIAA1217 | KIAA1217 | XM_011519555.3 | 43.3% | 42.4% | (many diffs) |
| 33 | human | 56243 | KIAA1217 | KIAA1217 | XM_011519552.3 | 42.7% | 41.8% | (many diffs) |
| 34 | human | 56243 | KIAA1217 | KIAA1217 | XM_011519558.2 | 40.7% | 39.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2697
- ORF length:
- 2631
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggt tgttcctggc aatgccacca tccccaggga cagaatctcc agcctgccag 121 tctccagacc catctctcca agcccaagcg ccattttaga aagaagagat gtcaagcctg 181 atgaagacat gagtggcaaa aacattgcaa tgtacagaaa tgagggtttc tatgctgatc 241 cttaccttta tcacgaggga cggatgagca tagcctcatc ccatggtgga cacccactgg 301 atgtccccga ccacatcatt gcatatcacc gcaccgccat ccggtcagcg agtgcttatt 361 gtaacccctc aatgcaagcg gaaatgcata tggaacaatc actgtacaga cagaaatcaa 421 ggaaatatcc ggatagccat ttgcctacac tgggctccaa aacaccccct gcctctcctc 481 acagagtcag tgacctgagg atgatagaca tgcacgctca ctataatgcc cacggccccc 541 ctcacaccat gcagccagac cgggcctctc cgagccgcca ggcctttaaa aaggagccag 601 gcaccttggt gtatatagaa aagccacgga gcgctgcagg attatccagc cttgtagacc 661 tcggccctcc tctaatggag aagcaagttt ttgcctacag cacggcgaca atacccaaag 721 acagagagac cagcgagaaa atgatgaaaa ccacagccaa caggaaccac acagatagtg 781 caggaacgcc ccatgtgtct ggtgggaaga tgctcagtgc tctggagtcc acggtgcctc 841 ccagccagcc tccacctgtg ggcacctcag ccatccacat gagcctgctt gagatgaggc 901 ggagcgtggc ggaactcagg ctccagctcc agcagatgcg gcagctccag ctgcagaacc 961 aggagttgct gagggcaatg atgaagaagg ccgagctgga aatcagtggc aaagtgatgg 1021 aaacaatgaa gagactggag gatcccgtgc agcgacagcg cgtcctagtg gagcaagaga 1081 gacaaaaata tcttcatgag gaagagaaga tcgtcaagaa gttgtgcgag ttggaagact 1141 ttgttgaaga cttgaagaag gactccacgg cagccagccg attggttact ctgaaagacg 1201 tggaagacgg ggctttcctc ctgcgtcaag tgggagaggc tgtagctacc ctgaaaggag 1261 aatttccaac cttacaaaac aagatgcgag ccatcctgcg catagaagtg gaggccgtgc 1321 ggtttctgaa ggaggagcca cacaagctgg acagtctcct gaagcgtgtg cgcagcatga 1381 cagacgtcct gaccatgctg cggagacatg tcactgatgg gctcctgaaa ggcacggacg 1441 cagcccaagc cgcacagtac atggctatgg aaaaggccac agccgcagaa gtcctgaaga 1501 gtcaggagga ggcagcccac acctccggcc agcccttcca cagcacaggt gcccctggcg 1561 atgcgaagtc ggaagtggtg cctttgtccg gcatgatggt tcgccacgcg cagagctccc 1621 ctgtggtcat ccagccctcc cagcactccg tggccctgct gaaccctgct cagaacttgc 1681 ctcacgtggc cagctcccca gccgtccccc aggaagcaac ctccactctg cagatgtcgc 1741 aggctccgca gtccccacag atacccatga atgggtctgc catgcagagc ttgttcattg 1801 aagaaatcca cagtgtgagt gccaagaaca gggcagtgtc tatcgagaaa gcagaaaaga 1861 aatgggagga aaaaaggcaa aatctggatc actataatgg gaaagagttt gagaagctcc 1921 tagaagaagc tcaggccaat atcatgaagt caataccaaa tctggagatg ccgccagcca 1981 caggcccact gccaagggga gatgccccag tggacaaggt ggaactttca gaagattctc 2041 caaattcgga acaggacttg gaaaagctgg ggggaaagtc gccccctcct cctccgccac 2101 ctcctcgtcg aagctacctg ccaggatcgg gactcaccac cacgaggtca ggcgatgtgg 2161 tctacaccgg cagaaaggag aacatcaccg ctaaggcaag cagtgaagat gctggaccaa 2221 gcccacagac cagagctaca aaatatccag cagaggagcc tgcttcagcc tggaccccat 2281 ccccaccgcc tgtcaccacc tcctcctcaa aggatgagga ggaagaagaa gaagaaggag 2341 acaaaataat ggcagaactc caggcattcc agaagtgttc ctttatggat gtaaattcaa 2401 acagtcatgc tgagccatcc cgggctgaca gtcacgttaa agacactagg tcgggcgcca 2461 cagtgccacc caaggagaag aagaatttgg aatttttcca tgaagatgta cggaaatctg 2521 atgttgaata tgaaaatggc ccccaaatgg aattccaaaa gggctccagc ggggccccac 2581 agacgagcag gatgcctgtc cccatgagtg ccaagaacag acccggaacc ctggacaaac 2641 ccggcaagca gtccaaactg caggatcccc gccaatatcg tcaggtagtt ttaccttacc 2701 caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt 2761 ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga 2821 tta