Construct: ORF ccsbBroad304_12310
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013306.1_s304c1, BRDN0000402700
- Derived from:
- ccsbBroadEn_12310
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SPIRE1 (56907)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12310
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56907 | SPIRE1 | spire type actin nucleation... | NM_001128627.1 | 21% | 21% | 1_1473del |
| 2 | human | 56907 | SPIRE1 | spire type actin nucleation... | XM_024451225.1 | 20.6% | 20.6% | 1_1509del |
| 3 | human | 56907 | SPIRE1 | spire type actin nucleation... | XM_011525702.1 | 18.8% | 18.8% | 1_1689del |
| 4 | human | 56907 | SPIRE1 | spire type actin nucleation... | XM_024451224.1 | 18.8% | 18.8% | 1_1689del |
| 5 | human | 56907 | SPIRE1 | spire type actin nucleation... | NM_020148.3 | 17.6% | 17.6% | 1_1833del |
| 6 | human | 56907 | SPIRE1 | spire type actin nucleation... | NM_001128626.2 | 17.3% | 17.3% | 1_1875del |
| 7 | human | 56907 | SPIRE1 | spire type actin nucleation... | XM_005258122.4 | 16.3% | 16.3% | 1_2007del |
| 8 | human | 56907 | SPIRE1 | spire type actin nucleation... | XM_011525701.1 | 16% | 16% | 1_2049del |
| 9 | mouse | 68166 | Spire1 | spire homolog 1 (Drosophila) | NM_176832.2 | 22.1% | 23.8% | (many diffs) |
| 10 | mouse | 68166 | Spire1 | spire homolog 1 (Drosophila) | NM_194355.2 | 17.7% | 19.1% | (many diffs) |
| 11 | mouse | 68166 | Spire1 | spire homolog 1 (Drosophila) | XM_006526207.3 | 15.6% | 16.8% | (many diffs) |
| 12 | mouse | 68166 | Spire1 | spire homolog 1 (Drosophila) | XM_006526206.1 | 15.3% | 16.5% | (many diffs) |
| 13 | mouse | 68166 | Spire1 | spire homolog 1 (Drosophila) | XM_006526205.1 | 15.3% | 16.5% | (many diffs) |
| 14 | mouse | 68166 | Spire1 | spire homolog 1 (Drosophila) | XM_006526204.3 | 14.4% | 15.6% | (many diffs) |
| 15 | mouse | 68166 | Spire1 | spire homolog 1 (Drosophila) | XM_006526203.3 | 14.2% | 15.3% | (many diffs) |
| 16 | mouse | 68166 | Spire1 | spire homolog 1 (Drosophila) | XM_006526209.3 | 14.2% | 15.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 459
- ORF length:
- 393
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgcg gctgccctcc aaaccatact ccactcttcc tatcttttca ttgggacctt 121 ctgctctgca aagaggggaa agtagtatga ggtcagaaaa accctccact gcccatcatc 181 ggccacttcg gagcattgcc aggttctcct caaaatctaa gtctatggac aaatcagatg 241 aagaactcca gtttcccaaa gagttgatgg aggactggag caccatggag gtgtgtgtgg 301 actgcaagaa gttcatttcg gaaatcatca gttcaagccg gcgcagtctg gtgttggcca 361 acaaaagggc ccgattgaaa aggaaaacgc agtctttcta catgtcctcg ccaggcccct 421 cggagtactg cccttcagag aggacgatca gtgagatctg cccaactttc ttgtacaaag 481 tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta 541 gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta