Construct: ORF ccsbBroad304_12318
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009722.1_s304c1, BRDN0000460619
- Derived from:
- ccsbBroadEn_12318
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MFF (56947)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12318
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56947 | MFF | mitochondrial fission factor | NM_001277064.2 | 100% | 100% | |
| 2 | human | 56947 | MFF | mitochondrial fission factor | XM_006712639.3 | 100% | 100% | |
| 3 | human | 56947 | MFF | mitochondrial fission factor | NM_001277068.1 | 94.4% | 94.4% | 441_482del |
| 4 | human | 56947 | MFF | mitochondrial fission factor | NM_001277063.2 | 91.9% | 90.6% | (many diffs) |
| 5 | human | 56947 | MFF | mitochondrial fission factor | XM_017004515.2 | 91.9% | 90.6% | (many diffs) |
| 6 | human | 56947 | MFF | mitochondrial fission factor | NM_001277065.2 | 91.5% | 91.5% | 440_441ins60 |
| 7 | human | 56947 | MFF | mitochondrial fission factor | NM_001277066.2 | 91.5% | 91.5% | 440_441ins60 |
| 8 | human | 56947 | MFF | mitochondrial fission factor | XM_005246687.4 | 90.1% | 90.1% | 1_78del |
| 9 | human | 56947 | MFF | mitochondrial fission factor | XM_017004513.2 | 86.3% | 82.3% | (many diffs) |
| 10 | human | 56947 | MFF | mitochondrial fission factor | XM_017004514.2 | 86.3% | 82.3% | (many diffs) |
| 11 | human | 56947 | MFF | mitochondrial fission factor | XM_005246686.4 | 83.2% | 82% | (many diffs) |
| 12 | human | 56947 | MFF | mitochondrial fission factor | XM_005246688.2 | 82.5% | 82.5% | 1_78del;518_519ins60 |
| 13 | human | 56947 | MFF | mitochondrial fission factor | XM_005246685.4 | 82.3% | 82.3% | 1_78del;519_593del |
| 14 | human | 56947 | MFF | mitochondrial fission factor | XM_024453009.1 | 82.3% | 82.3% | 1_78del;519_593del |
| 15 | human | 56947 | MFF | mitochondrial fission factor | NM_001277062.2 | 81.7% | 81.7% | 441_599del |
| 16 | human | 56947 | MFF | mitochondrial fission factor | XM_006712638.2 | 81.7% | 81.7% | 441_599del |
| 17 | human | 56947 | MFF | mitochondrial fission factor | XM_024453008.1 | 81.7% | 81.7% | 441_599del |
| 18 | human | 56947 | MFF | mitochondrial fission factor | XM_005246684.2 | 78.8% | 75.1% | (many diffs) |
| 19 | human | 56947 | MFF | mitochondrial fission factor | XM_005246683.2 | 75.3% | 75.3% | 441_674del |
| 20 | human | 56947 | MFF | mitochondrial fission factor | XM_006712637.2 | 75.3% | 75.3% | 441_674del |
| 21 | human | 56947 | MFF | mitochondrial fission factor | XM_011511501.1 | 75.3% | 75.3% | 441_674del |
| 22 | human | 56947 | MFF | mitochondrial fission factor | XM_005246682.2 | 75% | 75% | 1_78del;519_677del |
| 23 | human | 56947 | MFF | mitochondrial fission factor | XM_017004512.1 | 75% | 75% | 1_78del;519_677del |
| 24 | human | 56947 | MFF | mitochondrial fission factor | XM_005246681.4 | 72.2% | 69.5% | (many diffs) |
| 25 | human | 56947 | MFF | mitochondrial fission factor | NM_001277061.2 | 69.5% | 69.5% | 1_78del;519_752del |
| 26 | human | 56947 | MFF | mitochondrial fission factor | NM_020194.5 | 69.5% | 69.5% | 1_78del;519_752del |
| 27 | human | 56947 | MFF | mitochondrial fission factor | XM_011511500.1 | 69.5% | 69.5% | 1_78del;519_752del |
| 28 | human | 56947 | MFF | mitochondrial fission factor | NM_001277067.1 | 67.9% | 63.9% | (many diffs) |
| 29 | human | 644762 | LOC644762 | mitochondrial fission facto... | NR_110534.1 | 37.3% | (many diffs) | |
| 30 | human | 56947 | MFF | mitochondrial fission factor | NR_102266.2 | 35.5% | (many diffs) | |
| 31 | mouse | 75734 | Mff | mitochondrial fission factor | NM_001310697.1 | 91% | 94.1% | (many diffs) |
| 32 | mouse | 75734 | Mff | mitochondrial fission factor | NM_001310695.1 | 90.6% | 93.7% | (many diffs) |
| 33 | mouse | 75734 | Mff | mitochondrial fission factor | NM_001372413.1 | 87% | 89.9% | (many diffs) |
| 34 | mouse | 75734 | Mff | mitochondrial fission factor | NM_001372412.1 | 86.6% | 89.6% | (many diffs) |
| 35 | mouse | 75734 | Mff | mitochondrial fission factor | NM_001310699.1 | 82.9% | 85.7% | (many diffs) |
| 36 | mouse | 75734 | Mff | mitochondrial fission factor | NM_001372414.1 | 82.5% | 85.3% | (many diffs) |
| 37 | mouse | 75734 | Mff | mitochondrial fission factor | XM_006496567.4 | 82% | 84.8% | (many diffs) |
| 38 | mouse | 75734 | Mff | mitochondrial fission factor | XM_006496566.4 | 81.7% | 84.5% | (many diffs) |
| 39 | mouse | 75734 | Mff | mitochondrial fission factor | XM_006496565.4 | 78.7% | 81.4% | (many diffs) |
| 40 | mouse | 75734 | Mff | mitochondrial fission factor | XM_006496564.3 | 78.5% | 81.1% | (many diffs) |
| 41 | mouse | 75734 | Mff | mitochondrial fission factor | NM_001372411.1 | 77.4% | 77.2% | (many diffs) |
| 42 | mouse | 75734 | Mff | mitochondrial fission factor | NM_001372409.1 | 77.1% | 76.9% | (many diffs) |
| 43 | mouse | 75734 | Mff | mitochondrial fission factor | XM_006496569.3 | 74.7% | 77.2% | (many diffs) |
| 44 | mouse | 75734 | Mff | mitochondrial fission factor | NM_029409.3 | 74.6% | 76.9% | (many diffs) |
| 45 | mouse | 75734 | Mff | mitochondrial fission factor | XM_006496568.4 | 74.4% | 76.9% | (many diffs) |
| 46 | mouse | 75734 | Mff | mitochondrial fission factor | NM_001372408.1 | 74.4% | 76.7% | (many diffs) |
| 47 | mouse | 75734 | Mff | mitochondrial fission factor | NM_001372407.1 | 71.9% | 74.1% | (many diffs) |
| 48 | mouse | 75734 | Mff | mitochondrial fission factor | NM_001372406.1 | 71.7% | 73.9% | (many diffs) |
| 49 | mouse | 75734 | Mff | mitochondrial fission factor | XM_006496563.4 | 70.7% | 70.4% | (many diffs) |
| 50 | mouse | 75734 | Mff | mitochondrial fission factor | XM_006496562.3 | 70.5% | 70.2% | (many diffs) |
| 51 | mouse | 75734 | Mff | mitochondrial fission factor | NM_001372405.1 | 68.5% | 70.6% | (many diffs) |
| 52 | mouse | 75734 | Mff | mitochondrial fission factor | XM_006496558.2 | 68.3% | 70.4% | (many diffs) |
| 53 | mouse | 75734 | Mff | mitochondrial fission factor | XM_006496557.4 | 66.2% | 68.2% | (many diffs) |
| 54 | mouse | 75734 | Mff | mitochondrial fission factor | XM_006496556.3 | 66% | 68% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 780
- ORF length:
- 714
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc agaaattagt cgaattcagt acgaaatgga atatactgaa ggcattagtc 121 agcgaatgag ggtcccagaa aagttaaaag tagcaccgcc aaacgctgac ctggaacaag 181 gattccaaga aggagttcca aatgctagtg tgataatgca agttccggag aggattgttg 241 tagcaggaaa taatgaagat gtttcatttt caagaccagc agatcttgac cttattcagt 301 caactccctt taaacccctg gcactgaaaa caccacctcg tgtacttacg ctgagtgaaa 361 gaccactaga ttttctggat ttagaaagac ctcctacaac ccctcaaaat gaagaaatcc 421 gagcagttgg cagactaaaa agagagcggt ctatgagtga aaatgctgtt cgccaaaatg 481 gacagctggt cagaaatgat tctctacctg tgttgcgtgg tgggtctgct gccgccactt 541 ctaatcctca tcatgacaac gtcaggtatg gcatttcaaa tatagataca accattgaag 601 gaacgtcaga tgacctgact gttgtagatg cagcttcact aagacgacag ataatcaaac 661 taaatagacg tctacaactt ctggaagagg agaacaaaga acgtgctaaa agagaaatgg 721 tcatgtattc aattactgta gctttctggc tgcttaatag ctggctctgg tttcgccgct 781 acccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg 841 attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct 901 ggatta