Construct: ORF ccsbBroad304_12343
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016288.1_s304c1, BRDN0000407944
- Derived from:
- ccsbBroadEn_12343
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CLK4 (57396)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12343
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57396 | CLK4 | CDC like kinase 4 | NM_020666.3 | 11.1% | 11% | 162_1443delinsG |
| 2 | mouse | 12750 | Clk4 | CDC like kinase 4 | NM_007714.6 | 9.9% | 10.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 228
- ORF length:
- 162
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgcg gcattccaaa agaactcact gtcctgattg ggatagcaga gaaagctggg 121 gacatgaaag ctatcgtgga agtcacaagc ggaagaggag atctcatagt agcacacaag 181 agaacaggca ttgtaaacca catcaccagt ttaaagaatc tgattggtac ccaactttct 241 tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt 301 aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta