Construct: ORF ccsbBroad304_12348
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015181.1_s304c1, BRDN0000408076
- Derived from:
- ccsbBroadEn_12348
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- CNOT6 (57472)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12348
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57472 | CNOT6 | CCR4-NOT transcription comp... | XR_001742164.1 | 4.5% | 1_3880del;4157_6028del | |
| 2 | human | 57472 | CNOT6 | CCR4-NOT transcription comp... | NR_163438.1 | 4.5% | 1_3916del;4193_6064del | |
| 3 | human | 57472 | CNOT6 | CCR4-NOT transcription comp... | NR_163437.1 | 4.3% | 1_4175del;4452_6323del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 67
- ORF end:
- 343
- ORF length:
- 276
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggtaatgt gcatcatgac aatttccagt gaaggtgagc tggagctggt tggactaatg 121 agactgagga agcagctttt cctacgatct gcattatgta atcacaggtc cagagagctt 181 tatggaagcg ggagaggagg agcacttact catgttgtat ttgttaatgg aggatgtcat 241 cttttcatag atgctggaac tagagtgcac ttgttagatg ctaaaggttt gagctttaca 301 caaaatgtct tcatctgtat ttgttattgt ctacaatata tttgaccaac tttcttgtac 361 aaagtggttg gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga 421 gctagcgcta accggtggcg cgttaagtcg acaatcaacc tctggatta