Construct: ORF ccsbBroad304_12356

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009293.1_s304c1, BRDN0000405114
Derived from:
ccsbBroadEn_12356
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
XPO5 (57510)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_12356
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 57510 XPO5 exportin 5 NM_020750.3 55.4% 55.4% 1_1608del
2 human 57510 XPO5 exportin 5 NR_144392.1 36.4% 1_1819del;2372_2509del;3962_5502del
3 mouse 72322 Xpo5 exportin 5 NM_028198.3 47.9% 48.4% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2070
ORF length:
2004
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggt tctgaacttt gataccaagg atcccctcat cctgtcctgc gtccttacta
121atgtctctgc actctttcca tttgtcacct acagaccaga gttcctgccc caggtcttct
181ctaagctatt ttcatctgtc acttttgaaa ctgttgaaga aagtaaggcc cccagaaccc
241gggcagtgag gaatgtgagg aggcatgctt gttcctccat catcaagatg tgtcgtgact
301acccccagct tgtgctgccc aattttgaca tgctttataa ccatgtgaag caactcctct
361ccaatgagct actcctgaca caaatggaga agtgtgccct catggaagcc ctggttctca
421ttagcaacca atttaagaac tacgagcgtc agaaggtgtt cctagaggag ctgatggcac
481cagtggccag catctggctt tctcaagaca tgcacagagt gctgtcagat gttgatgctt
541tcattgcgta tgtgggtaca gatcagaaga gctgtgaccc aggcctggag gatccgtgtg
601gcttaaaccg tgcacgaatg agcttttgtg tatacagcat tctgggtgtg gtgaaacgaa
661cttgctggcc cactgaccta gaagaggcca aagctggggg atttgtggtg ggttatacat
721ccagtggaaa tccaatcttc cgtaacccct gcacagagca gattctgaaa cttcttgaca
781atttgcttgc gcttataaga acccacaata cattatatgc accagaaatg ctagccaaaa
841tggcagagcc tttcaccaag gctctggata tgcttgacgc ggaaaaatct gctatattag
901gattacctca acctctcttg gaactcaatg actctcctgt cttcaaaacc gtcttggaaa
961gaatgcagcg tttcttctct accctctatg aaaactgttt tcatatccta gggaaggcag
1021gcccttccat gcagcaagac ttctatactg tggaggacct tgctacccag cttctcagct
1081cagcctttgt caacttgaac aatattcctg actaccgact cagacccatg cttcgtgtct
1141ttgtaaagcc tctggtgctc ttctgtcccc cagagcacta tgaagccctg gtatccccca
1201tcctcggacc tcttttcacc tacctccata tgaggctttc tcagaaatgg caagttatca
1261accaaaggag cctgctgtgt ggagaagatg aggctgcaga tgaaaaccca gagtctcaag
1321agatgctgga ggagcaactg gtgaggatgt taacccgaga agtcatggac ctaatcacgg
1381tttgctgtgt ttcaaagaag ggtgctgacc acagtagtgc tcccccagca gatggagacg
1441atgaagaaat gatggccaca gaggtcaccc cctcagctat ggcagagctt acagacctgg
1501gcaaatgtct gatgaagcat gaggatgttt gtacagcgct attaattaca gccttcaatt
1561ccctggcctg gaaagatact ctgtcctgcc agaggacaac ctcacagctc tgctggcctc
1621tcctcaaaca agtgctgtca gggacactgc tcgcagatgc agttacgtgg cttttcacca
1681gtgtgctgaa aggcttacag atgcacgggc agcacgacgg gtgcatggct tccctggtcc
1741atctggcctt ccagatatac gaggcactgc gccccaggta cctggagata agagctgtaa
1801tggagcaaat ccctgaaata cagaaggact cactggacca gtttgactgc aagcttttaa
1861acccctccct gcagaaagtg gctgacaagc gccgaaagga ccaattcaaa cgcctcattg
1921ctggttgcat tgggaaaccc ttgggagagc agttccgaaa agaagttcac attaagaatc
1981ttccctcact tttcaaaaaa acaaagccaa tgctggagac ggaggtgctg gacaatgatg
2041ggggtggcct ggccaccatc tttgaaccct gcccaacttt cttgtacaaa gtggttggta
2101agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc
2161ggtggcgcgt taagtcgaca atcaacctct ggatta

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