Construct: ORF ccsbBroad304_12360
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013036.1_s304c1, BRDN0000407762
- Derived from:
- ccsbBroadEn_12360
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TBC1D14 (57533)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12360
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57533 | TBC1D14 | TBC1 domain family member 14 | NM_001113361.1 | 97.7% | 97.8% | 1_45del;2067G>A |
| 2 | human | 57533 | TBC1D14 | TBC1 domain family member 14 | NM_020773.3 | 97.7% | 97.8% | 1_45del;2067G>A |
| 3 | human | 57533 | TBC1D14 | TBC1 domain family member 14 | XM_006713895.3 | 97.7% | 97.8% | 1_45del;2067G>A |
| 4 | human | 57533 | TBC1D14 | TBC1 domain family member 14 | XM_005247986.3 | 94.3% | 94.3% | 1_45del;1446_1447ins72;1995G>A |
| 5 | human | 57533 | TBC1D14 | TBC1 domain family member 14 | XM_017008477.1 | 94.3% | 94.3% | 1_45del;1446_1447ins72;1995G>A |
| 6 | human | 57533 | TBC1D14 | TBC1 domain family member 14 | XM_011513507.3 | 93.2% | 93.2% | 1_45del;1447_1548del;2169G>A |
| 7 | human | 57533 | TBC1D14 | TBC1 domain family member 14 | XM_011513508.1 | 93.2% | 93.2% | 1_45del;1447_1548del;2169G>A |
| 8 | human | 57533 | TBC1D14 | TBC1 domain family member 14 | NM_001330638.2 | 67.3% | 67.1% | (many diffs) |
| 9 | human | 57533 | TBC1D14 | TBC1 domain family member 14 | NM_001113363.2 | 60.8% | 60.7% | 0_1ins795;2T>A;1227G>A |
| 10 | human | 57533 | TBC1D14 | TBC1 domain family member 14 | XM_011513513.2 | 57.9% | 57.8% | (many diffs) |
| 11 | human | 57533 | TBC1D14 | TBC1 domain family member 14 | XM_017008479.1 | 57.2% | 57.2% | (many diffs) |
| 12 | human | 57533 | TBC1D14 | TBC1 domain family member 14 | NM_001286805.1 | 52% | 52% | 0_1ins975;1047G>A |
| 13 | human | 57533 | TBC1D14 | TBC1 domain family member 14 | XM_017008480.1 | 49.5% | 49.5% | 0_1ins975;427_528del;1149G>A |
| 14 | human | 57533 | TBC1D14 | TBC1 domain family member 14 | XR_241653.1 | 35.6% | (many diffs) | |
| 15 | mouse | 100855 | Tbc1d14 | TBC1 domain family, member 14 | NM_133910.3 | 84.8% | 88.7% | (many diffs) |
| 16 | mouse | 100855 | Tbc1d14 | TBC1 domain family, member 14 | XM_006503641.3 | 84.8% | 88.7% | (many diffs) |
| 17 | mouse | 100855 | Tbc1d14 | TBC1 domain family, member 14 | XM_006503642.3 | 84.8% | 88.7% | (many diffs) |
| 18 | mouse | 100855 | Tbc1d14 | TBC1 domain family, member 14 | NM_001113362.1 | 82.4% | 86.2% | (many diffs) |
| 19 | mouse | 100855 | Tbc1d14 | TBC1 domain family, member 14 | XM_006503640.3 | 82.4% | 86.2% | (many diffs) |
| 20 | mouse | 100855 | Tbc1d14 | TBC1 domain family, member 14 | XM_006503644.3 | 60.1% | 63.7% | (many diffs) |
| 21 | mouse | 100855 | Tbc1d14 | TBC1 domain family, member 14 | NM_001113364.1 | 54.5% | 58.7% | (many diffs) |
| 22 | mouse | 100855 | Tbc1d14 | TBC1 domain family, member 14 | XM_006503645.1 | 54.5% | 58.7% | (many diffs) |
| 23 | mouse | 100855 | Tbc1d14 | TBC1 domain family, member 14 | XM_006503647.3 | 46.5% | 50.1% | (many diffs) |
| 24 | mouse | 100855 | Tbc1d14 | TBC1 domain family, member 14 | XR_376793.2 | 36% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2100
- ORF length:
- 2034
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggg tattctggat ggtcgaccag gaaaccccct tcagaacctg caacacgtca 121 atctcaaggc gccccgactc ctctccgcgc ctgagtacgg gcccaagctg aaactcaggg 181 ctttagaaga ccggcacagc ctccagtccg tggactcggg gattcctacc ctggagatcg 241 ggaacccgga gcctgtaccc tgcagcgcgg tccacgtgag gaggaagcag tccgactccg 301 acctcatccc cgagcgggcc ttccagagcg cctgcgcgct gccatcctgt gcgccaccag 361 ctcctagcag caccgagcgg gaacagagcg tgcgcaaatc ctccacgttt cccaggacag 421 gctatgactc ggtaaagctc tatagcccga cctccaaagc cctgacccgc agcgatgatg 481 tctccgtctg cagcgtgtcc agtcttggga cagagctgtc caccacgctg tccgtcagca 541 atgaggacat cttggacctt gtggtcacga gcagctccag tgccattgtg accctggaga 601 atgacgatga cccacagttt accaacgtca ccttgagctc tatcaaggaa acccgtggct 661 tacaccagca ggactgtgtt catgaagctg aggaggggag taaattgaaa atattggggc 721 catttagtaa cttctttgca aggaacttgc ttgctagaaa acaaagtgca aggcttgaca 781 aacacaatga cttgggatgg aagttatttg ggaaagcgcc actccgagag aatgcccaga 841 aggattcaaa gagaatacag aaggaatatg aagacaaggc tggaagacct agcaagccac 901 cctctccaaa gcagaatgtg aggaagaatc ttgactttga accactttcc accaccgcac 961 tcatcctcga ggacagacca gcaaatctcc cagcaaaacc agctgaagaa gctcagaagc 1021 acagacagca gtatgaagaa atggtggttc aggccaaaaa gcgagagctg aaagaagccc 1081 agcgaaggaa gaagcagctg gaagaaagat gcagagtcga ggaaagcatt ggaaacgctg 1141 tgctcacctg gaataatgag atcttaccta actgggaaac aatgtggtgc tctagaaaag 1201 ttcgagattt atggtggcag ggaatccctc caagtgtgag aggcaaagtc tggagcttag 1261 ccattggcaa cgagttaaat atcacccacg agctctttga catctgtctt gcccgagcca 1321 aggagaggtg gcggtccctt agcacaggag gctctgaagt ggagaacgaa gatgctggtt 1381 tttcagcagc agacagagaa gccagtctgg agcttattaa actggacatt tctagaacat 1441 ttcctaatct ctgcattttc cagcaaggtg gtccatatca tgacatgttg cacagtattt 1501 tgggcgctta tacttgttac cggccagatg tgggttatgt ccagggcatg tccttcatag 1561 cagcagtgtt gatcttgaac ttagatactg cagatgcctt tattgccttt tctaaccttc 1621 tgaataaacc ctgtcaaatg gcgtttttta gagtggacca tggccttatg ttgacttatt 1681 ttgctgcatt tgaagtattc tttgaagaaa atttgccgaa attatttgcg catttcaaga 1741 agaacaacct aactccagat atctacctaa ttgattggat ctttacctta tatagtaaat 1801 ctctgcccct cgacctggcc tgtcgtatct gggacgtgtt ctgtcgcgat ggggaagagt 1861 tcctgttccg cacggccctg ggcatcctga agctgttcga ggacatcctg accaagatgg 1921 acttcattca catggcccag ttcctgaccc ggctgcccga ggacctgccc gccgaggagc 1981 tgtttgcctc catcgccacg atccagatgc agagccgaaa caagaagtgg gctcaggtac 2041 tgactgcatt gcagaaagac agccgggaaa tggagaaggg aagtccatcc ctccgacact 2101 gcccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg 2161 attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct 2221 ggatta