Construct: ORF ccsbBroad304_12360

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013036.1_s304c1, BRDN0000407762
Derived from:
ccsbBroadEn_12360
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TBC1D14 (57533)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_12360
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 57533 TBC1D14 TBC1 domain family member 14 NM_001113361.1 97.7% 97.8% 1_45del;2067G>A
2 human 57533 TBC1D14 TBC1 domain family member 14 NM_020773.3 97.7% 97.8% 1_45del;2067G>A
3 human 57533 TBC1D14 TBC1 domain family member 14 XM_006713895.3 97.7% 97.8% 1_45del;2067G>A
4 human 57533 TBC1D14 TBC1 domain family member 14 XM_005247986.3 94.3% 94.3% 1_45del;1446_1447ins72;1995G>A
5 human 57533 TBC1D14 TBC1 domain family member 14 XM_017008477.1 94.3% 94.3% 1_45del;1446_1447ins72;1995G>A
6 human 57533 TBC1D14 TBC1 domain family member 14 XM_011513507.3 93.2% 93.2% 1_45del;1447_1548del;2169G>A
7 human 57533 TBC1D14 TBC1 domain family member 14 XM_011513508.1 93.2% 93.2% 1_45del;1447_1548del;2169G>A
8 human 57533 TBC1D14 TBC1 domain family member 14 NM_001330638.2 67.3% 67.1% (many diffs)
9 human 57533 TBC1D14 TBC1 domain family member 14 NM_001113363.2 60.8% 60.7% 0_1ins795;2T>A;1227G>A
10 human 57533 TBC1D14 TBC1 domain family member 14 XM_011513513.2 57.9% 57.8% (many diffs)
11 human 57533 TBC1D14 TBC1 domain family member 14 XM_017008479.1 57.2% 57.2% (many diffs)
12 human 57533 TBC1D14 TBC1 domain family member 14 NM_001286805.1 52% 52% 0_1ins975;1047G>A
13 human 57533 TBC1D14 TBC1 domain family member 14 XM_017008480.1 49.5% 49.5% 0_1ins975;427_528del;1149G>A
14 human 57533 TBC1D14 TBC1 domain family member 14 XR_241653.1 35.6% (many diffs)
15 mouse 100855 Tbc1d14 TBC1 domain family, member 14 NM_133910.3 84.8% 88.7% (many diffs)
16 mouse 100855 Tbc1d14 TBC1 domain family, member 14 XM_006503641.3 84.8% 88.7% (many diffs)
17 mouse 100855 Tbc1d14 TBC1 domain family, member 14 XM_006503642.3 84.8% 88.7% (many diffs)
18 mouse 100855 Tbc1d14 TBC1 domain family, member 14 NM_001113362.1 82.4% 86.2% (many diffs)
19 mouse 100855 Tbc1d14 TBC1 domain family, member 14 XM_006503640.3 82.4% 86.2% (many diffs)
20 mouse 100855 Tbc1d14 TBC1 domain family, member 14 XM_006503644.3 60.1% 63.7% (many diffs)
21 mouse 100855 Tbc1d14 TBC1 domain family, member 14 NM_001113364.1 54.5% 58.7% (many diffs)
22 mouse 100855 Tbc1d14 TBC1 domain family, member 14 XM_006503645.1 54.5% 58.7% (many diffs)
23 mouse 100855 Tbc1d14 TBC1 domain family, member 14 XM_006503647.3 46.5% 50.1% (many diffs)
24 mouse 100855 Tbc1d14 TBC1 domain family, member 14 XR_376793.2 36% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2100
ORF length:
2034
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggg tattctggat ggtcgaccag gaaaccccct tcagaacctg caacacgtca
121atctcaaggc gccccgactc ctctccgcgc ctgagtacgg gcccaagctg aaactcaggg
181ctttagaaga ccggcacagc ctccagtccg tggactcggg gattcctacc ctggagatcg
241ggaacccgga gcctgtaccc tgcagcgcgg tccacgtgag gaggaagcag tccgactccg
301acctcatccc cgagcgggcc ttccagagcg cctgcgcgct gccatcctgt gcgccaccag
361ctcctagcag caccgagcgg gaacagagcg tgcgcaaatc ctccacgttt cccaggacag
421gctatgactc ggtaaagctc tatagcccga cctccaaagc cctgacccgc agcgatgatg
481tctccgtctg cagcgtgtcc agtcttggga cagagctgtc caccacgctg tccgtcagca
541atgaggacat cttggacctt gtggtcacga gcagctccag tgccattgtg accctggaga
601atgacgatga cccacagttt accaacgtca ccttgagctc tatcaaggaa acccgtggct
661tacaccagca ggactgtgtt catgaagctg aggaggggag taaattgaaa atattggggc
721catttagtaa cttctttgca aggaacttgc ttgctagaaa acaaagtgca aggcttgaca
781aacacaatga cttgggatgg aagttatttg ggaaagcgcc actccgagag aatgcccaga
841aggattcaaa gagaatacag aaggaatatg aagacaaggc tggaagacct agcaagccac
901cctctccaaa gcagaatgtg aggaagaatc ttgactttga accactttcc accaccgcac
961tcatcctcga ggacagacca gcaaatctcc cagcaaaacc agctgaagaa gctcagaagc
1021acagacagca gtatgaagaa atggtggttc aggccaaaaa gcgagagctg aaagaagccc
1081agcgaaggaa gaagcagctg gaagaaagat gcagagtcga ggaaagcatt ggaaacgctg
1141tgctcacctg gaataatgag atcttaccta actgggaaac aatgtggtgc tctagaaaag
1201ttcgagattt atggtggcag ggaatccctc caagtgtgag aggcaaagtc tggagcttag
1261ccattggcaa cgagttaaat atcacccacg agctctttga catctgtctt gcccgagcca
1321aggagaggtg gcggtccctt agcacaggag gctctgaagt ggagaacgaa gatgctggtt
1381tttcagcagc agacagagaa gccagtctgg agcttattaa actggacatt tctagaacat
1441ttcctaatct ctgcattttc cagcaaggtg gtccatatca tgacatgttg cacagtattt
1501tgggcgctta tacttgttac cggccagatg tgggttatgt ccagggcatg tccttcatag
1561cagcagtgtt gatcttgaac ttagatactg cagatgcctt tattgccttt tctaaccttc
1621tgaataaacc ctgtcaaatg gcgtttttta gagtggacca tggccttatg ttgacttatt
1681ttgctgcatt tgaagtattc tttgaagaaa atttgccgaa attatttgcg catttcaaga
1741agaacaacct aactccagat atctacctaa ttgattggat ctttacctta tatagtaaat
1801ctctgcccct cgacctggcc tgtcgtatct gggacgtgtt ctgtcgcgat ggggaagagt
1861tcctgttccg cacggccctg ggcatcctga agctgttcga ggacatcctg accaagatgg
1921acttcattca catggcccag ttcctgaccc ggctgcccga ggacctgccc gccgaggagc
1981tgtttgcctc catcgccacg atccagatgc agagccgaaa caagaagtgg gctcaggtac
2041tgactgcatt gcagaaagac agccgggaaa tggagaaggg aagtccatcc ctccgacact
2101gcccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg
2161attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct
2221ggatta

Download FASTA (ORF) (Full)