Construct: ORF ccsbBroad304_12373

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF014987.1_s304c1, BRDN0000461496
Derived from:
ccsbBroadEn_12373
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
WDR48 (57599)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_12373
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 57599 WDR48 WD repeat domain 48 NM_001346227.2 100% 100%
2 human 57599 WDR48 WD repeat domain 48 NM_001303402.2 84.3% 84.3% 1_279del
3 human 57599 WDR48 WD repeat domain 48 NM_001346228.1 82.7% 82.7% 1_315del
4 human 57599 WDR48 WD repeat domain 48 NM_001346226.2 80.9% 80.9% 1_354del
5 human 57599 WDR48 WD repeat domain 48 XM_011533971.2 76.5% 75.3% (many diffs)
6 human 57599 WDR48 WD repeat domain 48 NM_001303403.2 75.1% 75.1% 1_498del
7 human 57599 WDR48 WD repeat domain 48 NM_020839.4 74.1% 74.1% 1_525del
8 human 57599 WDR48 WD repeat domain 48 NM_001346225.2 70.2% 70.2% 1_639del
9 human 57599 WDR48 WD repeat domain 48 XM_005265346.4 69% 67.8% (many diffs)
10 human 57599 WDR48 WD repeat domain 48 NR_144399.2 39.9% 1_405del;1912_3769del
11 human 57599 WDR48 WD repeat domain 48 NR_144400.2 38.6% 1_535del;2042_3899del
12 human 57599 WDR48 WD repeat domain 48 NR_144401.2 37.1% 1_535del;1482_1565del;2126_4049del
13 mouse 67561 Wdr48 WD repeat domain 48 NM_026236.3 65.4% 73.1% (many diffs)
14 mouse 67561 Wdr48 WD repeat domain 48 XM_006512246.2 64% 71.5% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1572
ORF length:
1506
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgaa tcaactggga acaatcattg tatcagggtc cactgaaaag gtgttacggg
121tatgggatcc aagaacatgt gcaaaactaa tgaagcttaa agggcacacg gataatgtga
181aggcattgct attaaacaga gatggcacgc aatgcctgtc aggcagttct gatgggacaa
241ttcgcctttg gtcccttggc cagcagagat gtatagcaac ataccgagtc catgatgaag
301gtgtttgggc gctgcaagtc aatgatgcct tcacacatgt gtattctggt ggaagggaca
361ggaagattta ttgtacagac ctaagaaacc ctgacattcg ggtgctaatt tgtgaagaaa
421aagcaccagt tctcaagatg gagcttgata gatcagctga tcctcctcct gcaatttggg
481ttgcaacaac taagtctaca gtaaataaat ggactttgaa aggaattcat aattttagag
541cctctggaga ttatgacaat gactgtacaa atcctataac acctctttgt acacaacctg
601accaggttat taaagggggt gctagtatta ttcagtgcca cattcttaat gataagagac
661atatattaac caaagatacc aataataatg tggcatattg ggatgtattg aaggcatgta
721aagttgaaga tctgggcaaa gtggattttg aagatgaaat taagaaaaga tttaaaatgg
781tgtatgtgcc aaattggttc tcagtagact taaaaacagg gatgttaact attactttgg
841atgaaagtga ttgttttgct gcctgggttt ctgcaaaaga tgctggtttc agcagccctg
901atgggtcaga tccaaaattg aatttaggag gacttttact ccaagcactc ctggaatatt
961ggcctagaac acatgtgaat ccaatggatg aagaggaaaa tgaagtaaac catgtaaatg
1021gggagcagga gaaccgagtg cagaagggaa atggatattt tcaagtgccc ccacatacac
1081ccgtgatctt tggtgaagct ggaggtcgca cactgttcag gctgctctgc cgagattccg
1141ggggtgagac tgagtctatg cttcttaatg aaacagtgcc acaatgggta attgacatca
1201ctgtggataa aaatatgccc aaattcaaca aaattccttt ctacctccaa cctcatgcat
1261cttcaggagc aaaaacctta aaaaaagata gactctctgc tagtgacatg ctccaagtcc
1321gaaaagttat ggaacatgtt tatgaaaaaa ttatcaactt ggataatgag tctcaaacca
1381ctagctcttc taataatgaa aaaccaggag aacaggaaaa agaagaagat attgctgtgt
1441tggcagagga gaaaattgaa cttttgtgcc aggaccaggt tttggatcca aatatggacc
1501ttcgaacagt gaaacacttc atatggaaga gcggtggaga cctcaccctc cattaccgtc
1561agaagtccac gtgcccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc
1621tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga
1681caatcaacct ctggatta

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