Construct: ORF ccsbBroad304_12373
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014987.1_s304c1, BRDN0000461496
- Derived from:
- ccsbBroadEn_12373
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- WDR48 (57599)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12373
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57599 | WDR48 | WD repeat domain 48 | NM_001346227.2 | 100% | 100% | |
| 2 | human | 57599 | WDR48 | WD repeat domain 48 | NM_001303402.2 | 84.3% | 84.3% | 1_279del |
| 3 | human | 57599 | WDR48 | WD repeat domain 48 | NM_001346228.1 | 82.7% | 82.7% | 1_315del |
| 4 | human | 57599 | WDR48 | WD repeat domain 48 | NM_001346226.2 | 80.9% | 80.9% | 1_354del |
| 5 | human | 57599 | WDR48 | WD repeat domain 48 | XM_011533971.2 | 76.5% | 75.3% | (many diffs) |
| 6 | human | 57599 | WDR48 | WD repeat domain 48 | NM_001303403.2 | 75.1% | 75.1% | 1_498del |
| 7 | human | 57599 | WDR48 | WD repeat domain 48 | NM_020839.4 | 74.1% | 74.1% | 1_525del |
| 8 | human | 57599 | WDR48 | WD repeat domain 48 | NM_001346225.2 | 70.2% | 70.2% | 1_639del |
| 9 | human | 57599 | WDR48 | WD repeat domain 48 | XM_005265346.4 | 69% | 67.8% | (many diffs) |
| 10 | human | 57599 | WDR48 | WD repeat domain 48 | NR_144399.2 | 39.9% | 1_405del;1912_3769del | |
| 11 | human | 57599 | WDR48 | WD repeat domain 48 | NR_144400.2 | 38.6% | 1_535del;2042_3899del | |
| 12 | human | 57599 | WDR48 | WD repeat domain 48 | NR_144401.2 | 37.1% | 1_535del;1482_1565del;2126_4049del | |
| 13 | mouse | 67561 | Wdr48 | WD repeat domain 48 | NM_026236.3 | 65.4% | 73.1% | (many diffs) |
| 14 | mouse | 67561 | Wdr48 | WD repeat domain 48 | XM_006512246.2 | 64% | 71.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1572
- ORF length:
- 1506
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgaa tcaactggga acaatcattg tatcagggtc cactgaaaag gtgttacggg 121 tatgggatcc aagaacatgt gcaaaactaa tgaagcttaa agggcacacg gataatgtga 181 aggcattgct attaaacaga gatggcacgc aatgcctgtc aggcagttct gatgggacaa 241 ttcgcctttg gtcccttggc cagcagagat gtatagcaac ataccgagtc catgatgaag 301 gtgtttgggc gctgcaagtc aatgatgcct tcacacatgt gtattctggt ggaagggaca 361 ggaagattta ttgtacagac ctaagaaacc ctgacattcg ggtgctaatt tgtgaagaaa 421 aagcaccagt tctcaagatg gagcttgata gatcagctga tcctcctcct gcaatttggg 481 ttgcaacaac taagtctaca gtaaataaat ggactttgaa aggaattcat aattttagag 541 cctctggaga ttatgacaat gactgtacaa atcctataac acctctttgt acacaacctg 601 accaggttat taaagggggt gctagtatta ttcagtgcca cattcttaat gataagagac 661 atatattaac caaagatacc aataataatg tggcatattg ggatgtattg aaggcatgta 721 aagttgaaga tctgggcaaa gtggattttg aagatgaaat taagaaaaga tttaaaatgg 781 tgtatgtgcc aaattggttc tcagtagact taaaaacagg gatgttaact attactttgg 841 atgaaagtga ttgttttgct gcctgggttt ctgcaaaaga tgctggtttc agcagccctg 901 atgggtcaga tccaaaattg aatttaggag gacttttact ccaagcactc ctggaatatt 961 ggcctagaac acatgtgaat ccaatggatg aagaggaaaa tgaagtaaac catgtaaatg 1021 gggagcagga gaaccgagtg cagaagggaa atggatattt tcaagtgccc ccacatacac 1081 ccgtgatctt tggtgaagct ggaggtcgca cactgttcag gctgctctgc cgagattccg 1141 ggggtgagac tgagtctatg cttcttaatg aaacagtgcc acaatgggta attgacatca 1201 ctgtggataa aaatatgccc aaattcaaca aaattccttt ctacctccaa cctcatgcat 1261 cttcaggagc aaaaacctta aaaaaagata gactctctgc tagtgacatg ctccaagtcc 1321 gaaaagttat ggaacatgtt tatgaaaaaa ttatcaactt ggataatgag tctcaaacca 1381 ctagctcttc taataatgaa aaaccaggag aacaggaaaa agaagaagat attgctgtgt 1441 tggcagagga gaaaattgaa cttttgtgcc aggaccaggt tttggatcca aatatggacc 1501 ttcgaacagt gaaacacttc atatggaaga gcggtggaga cctcaccctc cattaccgtc 1561 agaagtccac gtgcccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc 1621 tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga 1681 caatcaacct ctggatta