Construct: ORF ccsbBroad304_12487
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018012.1_s304c1, BRDN0000400330
- Derived from:
- ccsbBroadEn_12487
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CCDC14 (64770)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12487
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64770 | CCDC14 | coiled-coil domain containi... | NM_001308317.2 | 99.8% | 99.8% | 493A>C;1107T>C;1842C>T |
| 2 | human | 64770 | CCDC14 | coiled-coil domain containi... | NM_001366336.1 | 99.8% | 99.8% | 493A>C;1107T>C;1842C>T |
| 3 | human | 64770 | CCDC14 | coiled-coil domain containi... | NM_001366337.1 | 99.8% | 99.8% | 493A>C;1107T>C;1842C>T |
| 4 | human | 64770 | CCDC14 | coiled-coil domain containi... | XM_005247713.2 | 99.8% | 99.8% | 493A>C;1107T>C;1842C>T |
| 5 | human | 64770 | CCDC14 | coiled-coil domain containi... | NM_001366339.1 | 94.9% | 94.9% | (many diffs) |
| 6 | human | 64770 | CCDC14 | coiled-coil domain containi... | XM_024453715.1 | 94.8% | 94.8% | (many diffs) |
| 7 | human | 64770 | CCDC14 | coiled-coil domain containi... | NM_022757.5 | 87% | 87% | (many diffs) |
| 8 | human | 64770 | CCDC14 | coiled-coil domain containi... | NM_001366335.1 | 83% | 83% | (many diffs) |
| 9 | human | 64770 | CCDC14 | coiled-coil domain containi... | NM_001366338.1 | 80.8% | 80.8% | (many diffs) |
| 10 | human | 64770 | CCDC14 | coiled-coil domain containi... | XM_017007059.2 | 78.7% | 78.8% | 263_264ins477;630T>C;1365C>T |
| 11 | human | 64770 | CCDC14 | coiled-coil domain containi... | XM_006713731.3 | 63.9% | 63.9% | (many diffs) |
| 12 | human | 64770 | CCDC14 | coiled-coil domain containi... | XM_017007061.1 | 63.5% | 59.5% | (many diffs) |
| 13 | human | 64770 | CCDC14 | coiled-coil domain containi... | XM_005247711.4 | 62.2% | 62.3% | (many diffs) |
| 14 | human | 64770 | CCDC14 | coiled-coil domain containi... | XR_001740237.1 | 50.7% | (many diffs) | |
| 15 | human | 64770 | CCDC14 | coiled-coil domain containi... | XM_005247715.4 | 50.2% | 47.1% | (many diffs) |
| 16 | human | 64770 | CCDC14 | coiled-coil domain containi... | XM_011513081.2 | 49.5% | 47.5% | (many diffs) |
| 17 | human | 64770 | CCDC14 | coiled-coil domain containi... | XM_006713733.3 | 47.2% | 47.3% | (many diffs) |
| 18 | human | 64770 | CCDC14 | coiled-coil domain containi... | XM_005247716.4 | 33.8% | 33.7% | 1_600del;1093A>C;1569_1570ins1290 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2325
- ORF length:
- 2259
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgta ttcacccata atataccaag ccctctgtga gcacgtgcag actcagatgt 121 cactgatgaa tgacttgact tcaaagaaca tccctaatgg aattcctgct gtaccatgcc 181 atgctccctc tcattctgaa tctcaggcaa ctcctcattc tagttatggc ttatgtacct 241 ccaccccagt ctggtcactt cagcggccac cctgccctcc aaaggttcat tctgaagttc 301 aaactgatgg caacagtcag tttgcatcac aaggtaaaac agtttctgca acctgtactg 361 atgttctacg gaattcattt aataccagtc ctggagttcc atgtagcctg cccaaaactg 421 acatatcagc tattccaaca ttgcagcaac tgggccttgt taatggaatt ctgccacaac 481 aaggaattca taaggaaaca gacctactaa aatgtattca aacatatttg tctctttttc 541 gatctcatgg aaaagaaccg catctggaca gtcagacaca ccgaagccct actcagtcac 601 aaccagcttt cttggccact aatgaagaaa aatgtgccag agagcaaatt agagaggcca 661 caagtgaaag aaaggattta aacatacatg tgcgagatac aaaaacagtg aaggatgtac 721 agaaggcaaa aaatgtgaac aagacagctg aaaaagttag aattataaaa tatttgttgg 781 gagagctcaa ggccctggta gcagaacaag aggattcaga aattcagagg ttgattacag 841 aaatggaggc atgtatatct gtacttccaa cagtaagtgg aaacacagat attcaagttg 901 agatagcact ggccatgcaa ccattaagaa gtgagaatgc tcagttacga aggcagttga 961 gaattttgaa ccagcaactc agagaacaac agaaaactca aaaaccatct ggtgctgtgg 1021 attgcaacct tgaattgttt tctcttcagt cattgaatat gtcactgcaa aatcaattgg 1081 aggagtcact aaagagccag gaattactgc agagtaaaaa tgaagagctg ttaaaagtga 1141 ttgaaaatca gaaagatgaa aacaaaaaat tcagtagtat atttaaagac aaagatcaaa 1201 ctatacttga aaataaacag caatatgata ttgagataac aagaataaaa attgaattgg 1261 aggaagccct agtcaatgtg aaaagctccc agtttaagtt agaaactgct gaaaaggaaa 1321 accagatatt ggggataaca ttacgtcagc gtgatgctga ggtgactcga ctaagagaat 1381 taaccagaac tttacagact agcatggcaa agcttctctc cgatcttagt gtggacagtg 1441 ctcgctgcaa gcctgggaat aaccttacca aatcactctt gaacattcat gataaacaac 1501 ttcaacatga cccagctcct gctcacactt ccataatgag ctatctaaat aagttagaaa 1561 caaattacag ttttacacat tcagagccac tttctacaat taaaaatgag gaaaccatag 1621 agccagacaa aacctatgaa aatgttctgt cctccagagg ccctcaaaat agtaacacta 1681 ggggcatgga ggaagcatct gcacctggaa ttatttctgc cctttcaaaa caggattctg 1741 atgaagggag tgaaactatg gctttaatag aagatgagca taatttggat aatacaattt 1801 acattccttt tgctagaagc actcctgaaa agaaatcacc actttctaag agactatccc 1861 ctcagccaca aataagagca gctacaacac agctagtcag caacagtgga cttgctgtct 1921 ctggaaaaga aaataaactg tgtacacctg taatctgttc ctcttcaaca aaggaagcag 1981 aagatgcacc tgaaaaactt tccagagcat ctgatatgaa ggacacacag ctcctcaaga 2041 aaataaagga agcaattggt aagatccctg ctgccaccaa ggagccagag gaacaaactg 2101 catgtcatgg cccatcaggt tgtcttagca acagccttca agtgaaaggc aatactgtct 2161 gtgatggtag tgttttcact tctgacttga tgtctgactg gagcatctct tcgttttcaa 2221 cgttcacttc tcgtgatgaa caagacttca gaaatggcct tgcggcatta gatgccaaca 2281 tagctagact ccagaagtct ttaaggactg gtcttctgga gaaatgccca actttcttgt 2341 acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat 2401 gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a