Construct: ORF ccsbBroad304_12487

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018012.1_s304c1, BRDN0000400330
Derived from:
ccsbBroadEn_12487
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CCDC14 (64770)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_12487
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 64770 CCDC14 coiled-coil domain containi... NM_001308317.2 99.8% 99.8% 493A>C;1107T>C;1842C>T
2 human 64770 CCDC14 coiled-coil domain containi... NM_001366336.1 99.8% 99.8% 493A>C;1107T>C;1842C>T
3 human 64770 CCDC14 coiled-coil domain containi... NM_001366337.1 99.8% 99.8% 493A>C;1107T>C;1842C>T
4 human 64770 CCDC14 coiled-coil domain containi... XM_005247713.2 99.8% 99.8% 493A>C;1107T>C;1842C>T
5 human 64770 CCDC14 coiled-coil domain containi... NM_001366339.1 94.9% 94.9% (many diffs)
6 human 64770 CCDC14 coiled-coil domain containi... XM_024453715.1 94.8% 94.8% (many diffs)
7 human 64770 CCDC14 coiled-coil domain containi... NM_022757.5 87% 87% (many diffs)
8 human 64770 CCDC14 coiled-coil domain containi... NM_001366335.1 83% 83% (many diffs)
9 human 64770 CCDC14 coiled-coil domain containi... NM_001366338.1 80.8% 80.8% (many diffs)
10 human 64770 CCDC14 coiled-coil domain containi... XM_017007059.2 78.7% 78.8% 263_264ins477;630T>C;1365C>T
11 human 64770 CCDC14 coiled-coil domain containi... XM_006713731.3 63.9% 63.9% (many diffs)
12 human 64770 CCDC14 coiled-coil domain containi... XM_017007061.1 63.5% 59.5% (many diffs)
13 human 64770 CCDC14 coiled-coil domain containi... XM_005247711.4 62.2% 62.3% (many diffs)
14 human 64770 CCDC14 coiled-coil domain containi... XR_001740237.1 50.7% (many diffs)
15 human 64770 CCDC14 coiled-coil domain containi... XM_005247715.4 50.2% 47.1% (many diffs)
16 human 64770 CCDC14 coiled-coil domain containi... XM_011513081.2 49.5% 47.5% (many diffs)
17 human 64770 CCDC14 coiled-coil domain containi... XM_006713733.3 47.2% 47.3% (many diffs)
18 human 64770 CCDC14 coiled-coil domain containi... XM_005247716.4 33.8% 33.7% 1_600del;1093A>C;1569_1570ins1290
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2325
ORF length:
2259
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgta ttcacccata atataccaag ccctctgtga gcacgtgcag actcagatgt
121cactgatgaa tgacttgact tcaaagaaca tccctaatgg aattcctgct gtaccatgcc
181atgctccctc tcattctgaa tctcaggcaa ctcctcattc tagttatggc ttatgtacct
241ccaccccagt ctggtcactt cagcggccac cctgccctcc aaaggttcat tctgaagttc
301aaactgatgg caacagtcag tttgcatcac aaggtaaaac agtttctgca acctgtactg
361atgttctacg gaattcattt aataccagtc ctggagttcc atgtagcctg cccaaaactg
421acatatcagc tattccaaca ttgcagcaac tgggccttgt taatggaatt ctgccacaac
481aaggaattca taaggaaaca gacctactaa aatgtattca aacatatttg tctctttttc
541gatctcatgg aaaagaaccg catctggaca gtcagacaca ccgaagccct actcagtcac
601aaccagcttt cttggccact aatgaagaaa aatgtgccag agagcaaatt agagaggcca
661caagtgaaag aaaggattta aacatacatg tgcgagatac aaaaacagtg aaggatgtac
721agaaggcaaa aaatgtgaac aagacagctg aaaaagttag aattataaaa tatttgttgg
781gagagctcaa ggccctggta gcagaacaag aggattcaga aattcagagg ttgattacag
841aaatggaggc atgtatatct gtacttccaa cagtaagtgg aaacacagat attcaagttg
901agatagcact ggccatgcaa ccattaagaa gtgagaatgc tcagttacga aggcagttga
961gaattttgaa ccagcaactc agagaacaac agaaaactca aaaaccatct ggtgctgtgg
1021attgcaacct tgaattgttt tctcttcagt cattgaatat gtcactgcaa aatcaattgg
1081aggagtcact aaagagccag gaattactgc agagtaaaaa tgaagagctg ttaaaagtga
1141ttgaaaatca gaaagatgaa aacaaaaaat tcagtagtat atttaaagac aaagatcaaa
1201ctatacttga aaataaacag caatatgata ttgagataac aagaataaaa attgaattgg
1261aggaagccct agtcaatgtg aaaagctccc agtttaagtt agaaactgct gaaaaggaaa
1321accagatatt ggggataaca ttacgtcagc gtgatgctga ggtgactcga ctaagagaat
1381taaccagaac tttacagact agcatggcaa agcttctctc cgatcttagt gtggacagtg
1441ctcgctgcaa gcctgggaat aaccttacca aatcactctt gaacattcat gataaacaac
1501ttcaacatga cccagctcct gctcacactt ccataatgag ctatctaaat aagttagaaa
1561caaattacag ttttacacat tcagagccac tttctacaat taaaaatgag gaaaccatag
1621agccagacaa aacctatgaa aatgttctgt cctccagagg ccctcaaaat agtaacacta
1681ggggcatgga ggaagcatct gcacctggaa ttatttctgc cctttcaaaa caggattctg
1741atgaagggag tgaaactatg gctttaatag aagatgagca taatttggat aatacaattt
1801acattccttt tgctagaagc actcctgaaa agaaatcacc actttctaag agactatccc
1861ctcagccaca aataagagca gctacaacac agctagtcag caacagtgga cttgctgtct
1921ctggaaaaga aaataaactg tgtacacctg taatctgttc ctcttcaaca aaggaagcag
1981aagatgcacc tgaaaaactt tccagagcat ctgatatgaa ggacacacag ctcctcaaga
2041aaataaagga agcaattggt aagatccctg ctgccaccaa ggagccagag gaacaaactg
2101catgtcatgg cccatcaggt tgtcttagca acagccttca agtgaaaggc aatactgtct
2161gtgatggtag tgttttcact tctgacttga tgtctgactg gagcatctct tcgttttcaa
2221cgttcacttc tcgtgatgaa caagacttca gaaatggcct tgcggcatta gatgccaaca
2281tagctagact ccagaagtct ttaaggactg gtcttctgga gaaatgccca actttcttgt
2341acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat
2401gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a

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