Construct: ORF ccsbBroad304_12534

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF015643.1_s304c1, BRDN0000399421
Derived from:
ccsbBroadEn_12534
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DDX54 (79039)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_12534
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 79039 DDX54 DEAD-box helicase 54 NM_001111322.2 23.5% 21.5% (many diffs)
2 human 79039 DDX54 DEAD-box helicase 54 NM_024072.4 23.4% 21.3% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
738
ORF length:
672
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgca cctcggatgt ggagccggac acccgggaga tggtgcgtgc ccagaacaag
121aagaagaaga agtctggagg cttccagtcc atgggcctga gctacccggt gttcaaaggc
181atcatgaaga agggggagcc tttgagcagc aggcagctgg cgctgtcctg gacttgatgg
241gggatgaagc ccagaacctg acgaggggcc ggcagcagct caagtgggac cgtaagaaga
301agcggtttgt gggacagtca ggacaggaag acaagaagaa gattaagaca gagagcggcc
361gctacatcag cagctcctac aagcgagacc tctatcagaa gtggaaacag aaacagaaaa
421ttgatgatcg tgactcggac gaagaagggg catctgaccg gcgaggccca gagcgaagag
481gtgggaagcg agaccgtggc caagcaggtg catcccggcc ccacgcccca ggcacccctg
541caggccgagt ccgcccggaa ctcaagacca agcagcagat cctgaagcag cggcgccggg
601cccagaagct gcacttcctg cagcgtggtg gcctcaagca gctctctgcc cgcaaccgcc
661gccgcgtcca ggagctgcag cagggcgcct tcggccgggg tgcccgctcc aagaagggca
721agatgcggaa gaggatgtgc ccaactttct tgtacaaagt ggttggtaag cctatcccta
781accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta
841agtcgacaat caacctctgg atta

Download FASTA (ORF) (Full)