Construct: ORF ccsbBroad304_12535
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010076.1_s304c1, BRDN0000402372
- Derived from:
- ccsbBroadEn_12535
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TSEN34 (79042)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12535
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79042 | TSEN34 | tRNA splicing endonuclease ... | NM_001077446.4 | 79.2% | 68.3% | 334C>G;805delA;930_931ins241 |
| 2 | human | 79042 | TSEN34 | tRNA splicing endonuclease ... | NM_001282332.1 | 79.2% | 68.3% | 334C>G;805delA;930_931ins241 |
| 3 | human | 79042 | TSEN34 | tRNA splicing endonuclease ... | NM_024075.5 | 79.2% | 68.3% | 334C>G;805delA;930_931ins241 |
| 4 | human | 79042 | TSEN34 | tRNA splicing endonuclease ... | XM_011527294.3 | 79.2% | 68.3% | 334C>G;805delA;930_931ins241 |
| 5 | human | 79042 | TSEN34 | tRNA splicing endonuclease ... | XM_005278290.4 | 78.6% | 67.8% | (many diffs) |
| 6 | human | 79042 | TSEN34 | tRNA splicing endonuclease ... | NM_001282333.1 | 77% | 70.7% | (many diffs) |
| 7 | mouse | 66078 | Tsen34 | tRNA splicing endonuclease ... | NM_001164205.1 | 60.8% | 58% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1236
- ORF length:
- 1170
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgct ggtggtggag gtggcgaacg gccgctccct ggtgtgggga gccgaggcgg 121 tgcaggccct ccgggagcgc ctgggtgtgg ggggccgcac ggtaggcgcc ctgccccgcg 181 ggccccgcca gaactcgcgc ctgggcctcc cgctgctgct gatgcccgaa gaggcgcggc 241 tcttggccga gatcggcgcc gtgactctgg tcagcgcccc gcgtccagac tctcggcacc 301 acagcctggc cctgacatcc ttcaagcgcc agcaagagga gagcttccag gagcagagcg 361 ccttggcagc tgaggcccgg gagacccgtc gtcaggaggt cctggagaag attacggagg 421 gccaggctgc taagaagcag aaactagaac aggcttcagg ggccagctca agccaggagg 481 ccggctcgag ccaggctgcc aaagaggatg agaccagtga tggccaggct tcgggagagc 541 aggaggaagc tggcccctcg tcttcccaag caggaccctc aaatggggta gcccccttgc 601 ccagatctgc tctccttgtc cagctggcca ctgccaggcc tcgaccggtc aaggccaggc 661 ccctggactg gcgtgtccag tctaaagact ggccccacgc cggccgccct gcccacgagc 721 tgcgctacag tatctacaga gacctgtggg agcgaggctt cttcctcagt gcggctggca 781 agttcggagg tgacttcctg gtctatcctg gtgaccccct ccgcttccac gcccattata 841 tcgctcagtg ctgggcccct gaggacacct cccactccaa gacctggttg ctgctgggcg 901 ccttggaacc agcgtcagaa agaccctgct cctctgttct ccgcagcctg atggtaaggt 961 ggtctacacc tccctgcaat gggccagcct gcagtgaact ccagagacct aggggatgtg 1021 gctgtgtcgg cagcaagagc ctttctggat gttccccagc tcttctctgg gagtctagaa 1081 catcctccta cctttctccg cggttagttt ttgattccag gttttcgaac actacatctt 1141 ttttatgttc ttccttgttt caaagcactt attggctgtg tttttgtagt tacctatttt 1201 cacactgtga gcttcccgag aatggggcct gggtttgccc aactttcttg tacaaagtgg 1261 ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg 1321 ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta