Construct: ORF ccsbBroad304_12538
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011167.1_s304c1, BRDN0000411659
- Derived from:
- ccsbBroadEn_12538
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TRPM8 (79054)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12538
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79054 | TRPM8 | transient receptor potentia... | XM_024453134.1 | 75.6% | 75.6% | 0_1ins249;838_1167del |
| 2 | human | 79054 | TRPM8 | transient receptor potentia... | XM_024453133.1 | 73.6% | 72.2% | (many diffs) |
| 3 | human | 79054 | TRPM8 | transient receptor potentia... | XM_024453132.1 | 66.3% | 66.2% | (many diffs) |
| 4 | human | 79054 | TRPM8 | transient receptor potentia... | XM_017004891.1 | 64.9% | 63.7% | (many diffs) |
| 5 | human | 79054 | TRPM8 | transient receptor potentia... | NM_024080.5 | 61.6% | 61.5% | (many diffs) |
| 6 | human | 79054 | TRPM8 | transient receptor potentia... | XM_011511810.2 | 60.4% | 59.3% | (many diffs) |
| 7 | mouse | 171382 | Trpm8 | transient receptor potentia... | NM_134252.3 | 54.3% | 58% | (many diffs) |
| 8 | mouse | 171382 | Trpm8 | transient receptor potentia... | XM_006529225.1 | 54.3% | 58% | (many diffs) |
| 9 | mouse | 171382 | Trpm8 | transient receptor potentia... | XM_006529226.1 | 54.3% | 58% | (many diffs) |
| 10 | mouse | 171382 | Trpm8 | transient receptor potentia... | XM_006529229.1 | 50.4% | 50.8% | (many diffs) |
| 11 | mouse | 171382 | Trpm8 | transient receptor potentia... | XM_017319118.1 | 35.7% | 34.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2115
- ORF length:
- 2046
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gcaggccatc aatacctcca tcaaaaataa aattccttgt gtggtggtgg 121 aaggctcggg ccagatcgct gatgtgatcg ctagcctggt ggaggtggag gatgccctga 181 catcttctgc cgtcaaggag aagctggtgc gctttttacc ccgcacggtg tcccggctgc 241 ctgaggagga gactgagagt tggatcaaat ggctcaaaga aattctcgaa tgttctcacc 301 tattaacagt tattaaaatg gaagaagctg gggatgaaat tgtgagcaat gccatctcct 361 acgctctata caaagccttc agcaccagtg agcaagacaa ggataactgg aatgggcagc 421 tgaagcttct gctggagtgg aaccagctgg acttagccaa tgatgagatt ttcaccaatg 481 accgccgatg ggagtctgct gaccttcaag aagtcatgtt tacggctctc ataaaggaca 541 gacccaagtt tgtccgcctc tttctggaga atggcttgaa cctacggaag tttctcaccc 601 atgatgtcct cactgaactc ttctccaacc acttcagcac gcttgtgtac cggaatctgc 661 agatcgccaa gaattcctat aatgatgccc tcctcacgtt tgtctggaaa ctggttgcga 721 acttccgaag aggcttccgg aaggaagaca gaaatggccg ggacgagatg gacatagaac 781 tccacgacgt gtctcctatt actcggcacc ccctgcaagc tctcttcatc tgggccattc 841 ttcagaataa gaaggaactc tccaaagtca tttgggagca gaccaggggc tgcactctgg 901 cagccctggg agccagcaag cttctgaaga ctctggccaa agtgaagaac gacatcaatg 961 ctgctgggga gtccgaggag ctggctaatg agtacgagac ccgggctgtt gagctgttca 1021 ctgagtgtta cagcagcgat gaagacttgg cagaacagct gctggtctat tcctgtgaag 1081 cttggggtgg aagcaactgt ctggagctgg cggtggaggc cacagaccag catttcatcg 1141 cccagcctgg ggtccagtgg tacgtaaatg gggtgaatta ttttactgac ctgtggaatg 1201 tgatggacac gctggggctt ttttacttca tagcaggaat tgtatttcgg ctccactctt 1261 ctaataaaag ctctttgtat tctggacgag tcattttctg tctggactac attattttca 1321 ctctaagatt gatccacatt tttactgtaa gcagaaactt aggacccaag attataatgc 1381 tgcagaggat gctgatcgat gtgttcttct tcctgttcct ctttgcggtg tggatggtgg 1441 cctttggcgt ggccaggcaa gggatcctta ggcagaatga gcagcgctgg aggtggatat 1501 tccgttcggt catctacgag ccctacctgg ccatgttcgg ccaggtgccc agtgacgtgg 1561 atggtaccac gtatgacttt gcccactgca ccttcactgg gaatgagtcc aagccactgt 1621 gtgtggagct ggatgagcac aacctgcccc ggttccccga gtggatcacc atccccctgg 1681 tgtgcatcta catgttatcc accaacatcc tgctggtcaa cctgctggtc gccatgtttg 1741 gctacacggt gggcaccgtc caggagaaca atgaccaggt ctggaagttc cagaggtact 1801 tcctggtgca ggagtactgc agccgcctca atatcccctt ccccttcatc gtcttcgctt 1861 acttctacat ggtggtgaag aagtgcttca agtgttgctg caaggagaaa aacatggagt 1921 cttctgtctg ctgtttcaaa aatgaagaca atgagactct ggcatgggag ggtgtcatga 1981 aggaaaacta ccttgtcaag atcaacacaa aagccaacga cacctcagag gaaatgaggc 2041 atcgatttag acaactggat acaaagctta atgatctcaa gggtcttctg aaagagattg 2101 ctaataaaat caaattgcca actttcttgt acaaagtggt tggtaagcct atccctaacc 2161 ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt 2221 cgacaatcaa cctctggatt a