Construct: ORF ccsbBroad304_12565
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001574.1_s304c1, BRDN0000401146
- Derived from:
- ccsbBroadEn_12565
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- GLB1L (79411)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12565
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79411 | GLB1L | galactosidase beta 1 like | XM_024453139.1 | 100% | 100% | |
| 2 | human | 79411 | GLB1L | galactosidase beta 1 like | NM_001286427.1 | 72.8% | 72.8% | 1_459del |
| 3 | human | 79411 | GLB1L | galactosidase beta 1 like | XM_017004895.1 | 72.8% | 72.8% | 1_459del |
| 4 | human | 79411 | GLB1L | galactosidase beta 1 like | XM_024453138.1 | 72.8% | 72.8% | 1_459del |
| 5 | human | 79411 | GLB1L | galactosidase beta 1 like | NM_001286423.2 | 62.8% | 62.8% | 1_729del |
| 6 | human | 79411 | GLB1L | galactosidase beta 1 like | NM_024506.5 | 62.8% | 62.8% | 1_729del |
| 7 | human | 79411 | GLB1L | galactosidase beta 1 like | XM_024453137.1 | 62.8% | 62.8% | 1_729del |
| 8 | mouse | 74577 | Glb1l | galactosidase, beta 1-like | XR_373546.2 | 26.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1299
- ORF length:
- 1233
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttgacatgac caaaatcttt accctgcttc ggaagtatga accccatggg ccattggtaa 121 actctgagta ctacacaggc tggctggatt actggggcca gaatcactcc acacggtctg 181 tgtcagctgt aaccaaagga ctagagaaca tgctcaagtt gggagccagt gtgaacatgt 241 acatgttcca tggaggtacc aactttggat attggaatgg tgccgataag aagggacgct 301 tccttccgat tactaccagc tatgactatg atgcacctat atctgaagca ggggacccca 361 cacctaagct ttttgctctt cgagatgtca tcagcaagtt ccaggaagtt cctttgggac 421 ctttacctcc cccgagcccc aagatgatgc ttggacctgt gactctgcac ctggttgggc 481 atttactggc tttcctagac ttgctttgcc cccgtgggcc cattcattca atcttgccaa 541 tgacctttga ggctgtcaag caggaccatg gcttcatgtt gtaccgaacc tatatgaccc 601 ataccatttt tgagccaaca ccattctggg tgccaaataa tggagtccat gaccgtgcct 661 atgtgatggt ggatggggtg ttccagggtg ttgtggagcg aaatatgaga gacaaactat 721 ttttgacggg gaaactgggg tccaaactgg atatcttggt ggagaacatg gggaggctca 781 gctttgggtc taacagcagt gacttcaagg gcctgttgaa gccaccaatt ctggggcaaa 841 caatccttac ccagtggatg atgttccctc tgaaaattga taaccttgtg aagtggtggt 901 ttcccctcca gttgccaaaa tggccatatc ctcaagctcc ttctggcccc acattctact 961 ccaaaacatt tccaatttta ggctcagttg gggacacatt tctatatcta cctggatgga 1021 ccaagggcca agtctggatc aatgggttta acttgggccg gtactggaca aagcaggggc 1081 cacaacagac cctctacgtg ccaagattcc tgctgtttcc taggggagcc ctcaacaaaa 1141 ttacattgct ggaactagaa gatgtacctc tccagcccca agtccaattt ttggataagc 1201 ctatcctcaa tagcactagt actttgcaca ggacacatat caattccctt tcagctgata 1261 cactgagtgc ctctgaacca atggagttaa gtgggcactg accaactttc ttgtacaaag 1321 tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta 1381 gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta