Construct: ORF ccsbBroad304_12628
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017953.1_s304c1, BRDN0000403839
- Derived from:
- ccsbBroadEn_12628
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMC5 (79838)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12628
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79838 | TMC5 | transmembrane channel like 5 | NM_024780.5 | 85% | 85% | 1_339del;902T>C |
| 2 | human | 79838 | TMC5 | transmembrane channel like 5 | NM_001105248.1 | 64.2% | 64.2% | 1_1077del;1640T>C |
| 3 | human | 79838 | TMC5 | transmembrane channel like 5 | NM_001261841.2 | 64.2% | 64.2% | 1_1077del;1640T>C |
| 4 | human | 79838 | TMC5 | transmembrane channel like 5 | NM_001308161.1 | 59.1% | 59% | 1_1077del;1640T>C;1781_1782ins156 |
| 5 | human | 79838 | TMC5 | transmembrane channel like 5 | NM_001105249.1 | 58.5% | 58.4% | 1_1077del;1640T>C;2573_2574ins174 |
| 6 | mouse | 74424 | Tmc5 | transmembrane channel-like ... | NM_028930.3 | 72.1% | 72.8% | (many diffs) |
| 7 | mouse | 74424 | Tmc5 | transmembrane channel-like ... | XM_006508253.2 | 56.9% | 57.4% | (many diffs) |
| 8 | mouse | 74424 | Tmc5 | transmembrane channel-like ... | NM_001105252.1 | 56.5% | 57% | (many diffs) |
| 9 | mouse | 74424 | Tmc5 | transmembrane channel-like ... | XM_011241924.2 | 56.5% | 57% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2007
- ORF length:
- 1941
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgac atccagagac agaattaaag ccatcaggaa ccagccaagg accatggaag 121 agaaaaggaa ccttaggaaa atagttgaca aagaaaaaag caaacagacc catcgtatcc 181 ttcagctcaa ttgctgtatt cagtgtctga actccatttc ccgggcttat cggagatcca 241 agaacagcct gtcggaaatt ctgaattcca tcagcctgtg gcagaagacg ctgaagatca 301 ttggaggcaa gtttggaacc agcgtcctct cctatttcaa ctttctgaga tggcttttga 361 agttcaacat tttctcattc atcctgaact tcagcttcat cataatccct cagtttaccg 421 tggccaaaaa gaacaccctc cagttcactg ggctggagtt tttcactggg gtgggttatt 481 ttagggacac agtgatgtac tatggctttt acaccaattc caccatccag cacgggaaca 541 gcggggcatc ctacaacatg cagctggcct acatcttcac aatcggagca tgcttgacca 601 cctgcttctt cagtttgctg ttcagcacgg ccaagtattt ccggaacaac ttcattaatc 661 cccacattta ctccggaggg atcaccaagc tgatcttttg ctgggacttc actgtcactc 721 atgaaaaagc tgtgaagcta aaacagaaga atcttagcac tgagataagg gagaacctgt 781 cagagctccg tcaggagaat tccaagttga cgttcaatca gctgctgacc cgcttctctg 841 cctacatggt agcctgggtt gtctctacag gagtggccat agcctgctgt gcagccgttt 901 attacctggc tgagtacaac ttagagttcc tgaagacaca cagtaaccct ggggcggtgc 961 tgttactgcc tttcgttgtg tcctgcatta atctggccgt gccatgcatc tactccatgt 1021 tcaggcttgt ggagaggtac gagatgccac ggcacgaagt ctacgttctc ctgatccgaa 1081 acatcttttt gaaaatatca atcattggca ttctttgtta ctattggctc aacaccgtgg 1141 ccctgtctgg tgaagagtgt tgggaaaccc tcattggcca ggacatctac cggctccttc 1201 tgatggattt tgtgttctct ttagtcaatt ccttcctggg ggagtttctg aggagaatca 1261 ttgggatgca actgatcaca agtcttggcc ttcaggagtt tgacattgcc aggaacgttc 1321 tagaactgat ctatgcacaa actctggtgt ggattggcat cttcttctgc cccctgctgc 1381 cctttatcca aatgattatg cttttcatca tgttctactc caaaaatatc agcctgatga 1441 tgaatttcca gcctccgagc aaagcctggc gggcctcaca gatgatgact ttcttcatct 1501 tcttgctctt tttcccatcc ttcaccgggg tcttgtgcac cctggccatc accatctgga 1561 gattgaagcc ttcagctgac tgtggccctt ttcgaggtct gcctctcttc attcactcca 1621 tctacagctg gatcgacacc ctaagtacac ggcctggcta cctgtgggtt gtttggatct 1681 atcggaacct cattggaagt gtgcacttct ttttcatcct caccctcatt gtgctaatca 1741 tcacctatct ttactggcag atcacagagg gaaggaagat tatgataagg ctgctccatg 1801 agcagatcat taatgagggc aaagataaaa tgttcctgat agaaaaattg atcaagctgc 1861 aggatatgga gaagaaagca aaccccagct cacttgttct ggaaaggaga gaggtggagc 1921 aacaaggctt tttgcatttg ggggaacatg atggcagtct tgacttgcga tctagaagat 1981 cagttcaaga aggtaatcca agggcctgcc caactttctt gtacaaagtg gttggtaagc 2041 ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt 2101 ggcgcgttaa gtcgacaatc aacctctgga tta