Construct: ORF ccsbBroad304_12722

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002959.1_s304c1, BRDN0000461459
Derived from:
ccsbBroadEn_12722
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
COL21A1 (81578)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_12722
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 81578 COL21A1 collagen type XXI alpha 1 c... XM_024446561.1 99.8% 99.5% 908T>G;974G>A
2 human 81578 COL21A1 collagen type XXI alpha 1 c... NM_001318753.2 73.7% 73.1% (many diffs)
3 human 81578 COL21A1 collagen type XXI alpha 1 c... NM_001318754.2 65.2% 47.2% (many diffs)
4 human 81578 COL21A1 collagen type XXI alpha 1 c... NM_001318752.2 50.2% 50% (many diffs)
5 human 81578 COL21A1 collagen type XXI alpha 1 c... NM_001318751.2 50% 49.8% (many diffs)
6 human 81578 COL21A1 collagen type XXI alpha 1 c... NM_030820.4 50% 49.8% (many diffs)
7 human 81578 COL21A1 collagen type XXI alpha 1 c... XM_011514924.2 50% 49.8% (many diffs)
8 human 81578 COL21A1 collagen type XXI alpha 1 c... XM_011514925.3 50% 49.8% (many diffs)
9 human 81578 COL21A1 collagen type XXI alpha 1 c... XM_011514926.1 50% 49.8% (many diffs)
10 human 81578 COL21A1 collagen type XXI alpha 1 c... XM_011514927.1 50% 49.8% (many diffs)
11 human 81578 COL21A1 collagen type XXI alpha 1 c... XM_006715223.2 48.8% 48.5% (many diffs)
12 human 81578 COL21A1 collagen type XXI alpha 1 c... NR_134849.2 34.5% (many diffs)
13 human 81578 COL21A1 collagen type XXI alpha 1 c... NR_134850.2 34.3% (many diffs)
14 human 81578 COL21A1 collagen type XXI alpha 1 c... XR_001743657.2 34.2% (many diffs)
15 human 81578 COL21A1 collagen type XXI alpha 1 c... NR_134851.2 33.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1509
ORF length:
1440
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gggatatcag ggaattgcag ggacaccagg tgttccagga tctccaggaa
121tacaaggagc tcgaggacta ccaggttaca aaggagaacc agggcgagat ggtgacaagg
181gtgatcgtgg acttcctggt tttcctgggc ttcatggcat gccaggatca aagggtgaaa
241tgggtgccaa aggagacaaa ggatcacctg gattttatgg caaaaagggt gcaaaaggtg
301aaaaggggaa tgctggcttc cctggcctcc ctggacctgc tggagaacca ggaagacatg
361gaaaggatgg attaatgggt agtcccggtt tcaagggaga agcaggatcc cctggtgctc
421cggggcagga tggaacacgg ggagagcctg gaatcccagg atttcctgga aaccgaggat
481taatgggcca aaagggagaa attgggcctc caggacagca aggaaaaaaa ggagccccag
541ggatgcctgg tttaatggga agcaatggct caccaggcca gcctggaaca ccgggatcta
601agggaagcaa aggtgaacct ggaattcaag ggatgcctgg ggcttctggg ctcaagggag
661aaccaggagc aacgggttcc ccaggagaac caggatacat gggtttaccc gggattcaag
721gaaaaaaggg ggacaaagga aatcaaggtg aaaaaggtat tcagggtcaa aagggagaaa
781atggaagaca gggaattcca gggcaacagg gaattcaagg ccatcatggt gcaaaaggag
841agagaggtga aaagggagaa cctggtgtcc gaggtgccat tggatcaaaa ggagaatctg
901gggtggatgg cttgatgggg cccgcaggtc ctaaggggca acctggggat ccaggtcctc
961agggaccccc aggttgggat gggaagcccg gaagagagtt ttcagaacaa tttattcgac
1021aagtttgcac agatgtaata aaagcccagc taccagtctt acttcagagt ggaagaatta
1081gaaattgtga tcattgcctg tcccaacatg gctccccggg tattcctggg ccacctggtc
1141cgataggccc agagggtccc agaggattac ctggtttgcc aggaagagat ggtgttcctg
1201gattagtggg tgtccctgga cgtccaggtg tcagaggatt aaaaggccta ccaggaagaa
1261atggggaaaa agggagccaa gggtttgggt atcctggaga acaaggtcct cctggtcccc
1321caggtccaga gggccctcct ggaataagca aagaaggtcc tccaggagac ccaggtctcc
1381ctggcaaaga tggagaccat ggaaaacctg gaatccaagg gcaaccaggc cccccaggca
1441tctgcgaccc atcactatgt tttagtgtaa ttgccagaag agatccgttc agaaaaggac
1501caaactattt gccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc
1561tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa
1621tcaacctctg gatta

Download FASTA (ORF) (Full)