Construct: ORF ccsbBroad304_12752
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009241.1_s304c1, BRDN0000403930
- Derived from:
- ccsbBroadEn_12752
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CRISPLD2 (83716)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12752
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 83716 | CRISPLD2 | cysteine rich secretory pro... | NM_031476.4 | 89.2% | 85.9% | (many diffs) |
| 2 | human | 83716 | CRISPLD2 | cysteine rich secretory pro... | XM_005256190.1 | 89.2% | 85.9% | (many diffs) |
| 3 | human | 83716 | CRISPLD2 | cysteine rich secretory pro... | XM_024450463.1 | 82.3% | 81.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1413
- ORF length:
- 1347
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgag ctgcgtcctg ggtggtgtca tccccttggg gctgctgttc ctggtctgcg 121 gatcccaagg ctacctcctg cccaacgtca ctctcttaga ggagctgctc agcaaatacc 181 agcacaacga gtctcactcc cgggtccgca gagccatccc cagggaggac aaggaggaga 241 tcctcatgct gcacaacaag cttcggggcc aggtgcagcc tcaggcctcc aacatggagt 301 acatgacctg ggatgacgaa ctggagaagt ctgctgcagc gtgggccagt cagtgcatct 361 gggagcacgg gcccaccggt ctgctggtgt ccatcgggca gaacctgggc gctcactggg 421 gcaggtatcg ctctccgggg ttccatgtgc agtcctggta tgacgaggtg aaggactaca 481 cctaccccta cccgagcgag tgcaacccct ggtgtccaga gaggtgctcg gggcctatgt 541 gcacgcacta cacacagata gtttgggcca ccaccaacaa gatcggttgt gctgtgaaca 601 cctgccggaa gatgactgtc tggggagaag tttgggagaa cgcggtctac tttgtctgca 661 attattctcc aaaggggaac tggattggag aagcccccta caagaatggc cggccctgct 721 ctgagtgccc acccagctat ggaggcagct gcaggaacaa cttgtgttac cgagaagaaa 781 cctacactcc aaaacctgaa acggacgaga tgaatgaggt ggaaacggct cccattcctg 841 aagaaaacca tgtttggctc caaccgaggg tgatgagacc caccaagccc aagaaaacct 901 ctgcggtcaa ctacatgacc caagtcgtca gatgtgacac caagatgaag gacaggtgca 961 aagggtccac gtgtaacagg taccagtgcc cagcaggctg cctgaaccac aaggcgaaga 1021 tctttggaag tctgttctat gaaagctcgt ctagcatatg ccgcgccgcc atccactacg 1081 ggatcctgga tgacaaggga ggcctggtgg atatcaccag gaacgggaag gtccccttct 1141 tcgtgaagtc tgagagacac ggcgtgcagt ccctcagcaa atacaaacct tccagctcat 1201 tcatggtgtc aaaagtgaaa gtgcaggatt tggactgcta cacgaccgtt gctcagctgt 1261 gcccgtttga aaagccagca actcactgcc caagaatcca ttgtccggca cactgcaaag 1321 acgaaccttc ctactgggct ccggtgtttg gaaccaacat ctatgcagat gtgagtagga 1381 tgcattttca acaactatct cgcaaagcct gttacccaac tttcttgtac aaagtggttg 1441 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta 1501 accggtggcg cgttaagtcg acaatcaacc tctggatta