Construct: ORF ccsbBroad304_12769
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001934.1_s304c1, BRDN0000410859
- Derived from:
- ccsbBroadEn_12769
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MAGT1 (84061)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12769
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84061 | MAGT1 | magnesium transporter 1 | NM_001367916.1 | 39.7% | 38.8% | (many diffs) |
| 2 | human | 84061 | MAGT1 | magnesium transporter 1 | NM_032121.5 | 36.2% | 35.4% | (many diffs) |
| 3 | mouse | 67075 | Magt1 | magnesium transporter 1 | NM_001190409.1 | 32% | 31.4% | (many diffs) |
| 4 | mouse | 67075 | Magt1 | magnesium transporter 1 | NM_025952.4 | 32% | 31.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 468
- ORF length:
- 402
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc agcgcgttgg cggttttggt gtgtctctgt gaccatggtg gtggcgctgc 121 tcatcgtttg cgacgttccc tcagcctctg cccaaagaaa gaaggagatg gtgttatctg 181 aaaaggttag tcagctgatg gaatggacta acaaaagacc tgtaataaga atgaatggag 241 acaagttccg tcgccttgtg aaagccccac cgagaaatta ctccgttatc gtcatgttca 301 ctgctctcca actgcataga cagtgtgtcg tttgcaagca agctgatgaa gaattccaga 361 tcctggcaaa ctcctggcga tactccagtg cattcaccaa caggatattt tttgccatgg 421 tggattttga tgaaggctct gatgtatttc agatgtttca ggttttttgc ccaactttct 481 tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt 541 aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta