Transcript: Mouse NM_025952.4

Mus musculus magnesium transporter 1 (Magt1), transcript variant 2, mRNA.

Source:
NCBI, updated 2017-05-27
Taxon:
Mus musculus (mouse)
Gene:
Magt1 (67075)
Length:
4115
CDS:
38..1144

Additional Resources:

NCBI RefSeq record:
NM_025952.4
NBCI Gene record:
Magt1 (67075)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_025952.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000110311 GCTCTCTATCTTTCGATCAAA pLKO.1 1087 CDS 100% 5.625 7.875 N Magt1 n/a
2 TRCN0000302768 GCTCTCTATCTTTCGATCAAA pLKO_005 1087 CDS 100% 5.625 7.875 N Magt1 n/a
3 TRCN0000064384 GCTACCTCTGACATGGATATT pLKO.1 1004 CDS 100% 13.200 9.240 N MAGT1 n/a
4 TRCN0000333179 GCTACCTCTGACATGGATATT pLKO_005 1004 CDS 100% 13.200 9.240 N MAGT1 n/a
5 TRCN0000110313 GCTCCAACTTTCATCAACTTT pLKO.1 542 CDS 100% 5.625 3.938 N Magt1 n/a
6 TRCN0000302767 GCTCCAACTTTCATCAACTTT pLKO_005 542 CDS 100% 5.625 3.938 N Magt1 n/a
7 TRCN0000110312 CCACCGAGAAATTACTCAGTT pLKO.1 338 CDS 100% 4.950 3.465 N Magt1 n/a
8 TRCN0000331856 CCACCGAGAAATTACTCAGTT pLKO_005 338 CDS 100% 4.950 3.465 N Magt1 n/a
9 TRCN0000110310 CCTGTGTCTTTCCACCTGAAA pLKO.1 1272 3UTR 100% 4.950 3.465 N Magt1 n/a
10 TRCN0000302766 CCTGTGTCTTTCCACCTGAAA pLKO_005 1272 3UTR 100% 4.950 3.465 N Magt1 n/a
11 TRCN0000110314 GCTCTCCAACTTCATAGACAA pLKO.1 374 CDS 100% 4.950 3.465 N Magt1 n/a
12 TRCN0000331631 GCTCTCCAACTTCATAGACAA pLKO_005 374 CDS 100% 4.950 3.465 N Magt1 n/a
13 TRCN0000120127 CCTGAGTTCAAATCCCAGCAA pLKO.1 2569 3UTR 100% 2.640 1.320 Y Adsl n/a
14 TRCN0000339691 CCTGAGTTCAAATCCCAGCAA pLKO_005 2569 3UTR 100% 2.640 1.320 Y Adsl n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_025952.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12768 pDONR223 100% 82.3% 85.6% None (many diffs) n/a
2 ccsbBroad304_12768 pLX_304 0% 82.3% 85.6% V5 (many diffs) n/a
3 TRCN0000467201 ACATTCACGGATTTCTCGACATCA pLX_317 39.5% 82.3% 85.6% V5 (many diffs) n/a
4 ccsbBroadEn_12769 pDONR223 100% 32% 31.4% None (many diffs) n/a
5 ccsbBroad304_12769 pLX_304 0% 32% 31.4% V5 (many diffs) n/a
6 TRCN0000466839 GTTCCCTCCCGCACGTGCTCACCC pLX_317 78.6% 32% 31.4% V5 (many diffs) n/a
Download CSV