Construct: ORF ccsbBroad304_12817
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005500.1_s304c1, BRDN0000462296
- Derived from:
- ccsbBroadEn_12817
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MAML2 (84441)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12817
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84441 | MAML2 | mastermind like transcripti... | NM_032427.4 | 99.7% | 99.7% | 1398T>C;1780_1788delCAGCAGCAG |
| 2 | human | 84441 | MAML2 | mastermind like transcripti... | XM_011543023.3 | 85.1% | 84.2% | (many diffs) |
| 3 | human | 84441 | MAML2 | mastermind like transcripti... | XM_011543024.3 | 79.9% | 80% | 0_1ins684;714T>C;1096_1104delCAGCAGCAG |
| 4 | human | 84441 | MAML2 | mastermind like transcripti... | XM_011543025.2 | 62.3% | 61.6% | (many diffs) |
| 5 | mouse | 270118 | Maml2 | mastermind like 2 (Drosophila) | NM_001013813.3 | 83% | 82.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3528
- ORF length:
- 3459
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gggggacaca gcgcccccgc aggcccccgc aggagggcta gggggggcct 121 ctggggcggg gctccttgga gggggctcag tcaccccgag agtgcacagt gctatcgtgg 181 agcgcctccg ggctcggatc gctgtctgcc gccaacacca cctgagctgt gaaggacgat 241 atgaacgagg tagggccgag agctcagacc gggaaagaga aagcaccttg cagctcctga 301 gccttgtaca gcatggccag ggggcaagga aagctggcaa acacaccaag gccaccgcca 361 ctgctgccac cactacagcc cctccaccgc cccctgctgc ccctcctgcg gcctcccaag 421 cagcagcaac agcagcccca ccgcccccac cagactatca ccatcaccac cagcagcacc 481 tgctgaacag tagcaataat ggtggcagtg gtgggataaa cggagagcag cagccgcccg 541 cttcaacccc aggggaccag aggaactcag ccctgattgc gctccagggt tccttgaaaa 601 gaaaacaggt agttaaccta tctcctgcca acagcaagcg acccaatggc tttgtggaca 661 actcatttct tgatatcaaa agaattcgtg ttggggagaa tctctctgca ggacaaggtg 721 gcctccaaat aaacaatgga caaagtcaga ttatgtcagg gaccttgcct atgagccaag 781 cacccctgcg aaagactaac actctgccat cccatacaca ttctcctggc aatggcctgt 841 ttaacatggg cttaaaggag gtaaagaagg agccaggaga gactctgtct tgcagtaagc 901 acatggatgg ccaaatgacc caagagaata tttttcctaa taggtacgga gacgaccctg 961 gagaacaact gatggatcct gagctgcagg aactgttcaa tgaactgacc aacatatctg 1021 tgcctcccat gagtgacctt gaactggaga acatgatcaa tgccaccata aagcaggatg 1081 acccatttaa cattgacttg ggtcagcaaa gccagaggag cacacctagg ccctccttac 1141 ccatggagaa aatagtgatc aaaagtgaat actcaccggg cttgactcag ggcccctcag 1201 gctctcctca gctgaggccc ccatcagctg gccccgcatt ctccatggcc aactctgccc 1261 tctccacttc gtctccaatc ccttcagtcc ctcagagcca ggctcagcct cagacaggct 1321 ccggagcaag ccgggccttg ccaagctggc aggaagtatc ccatgcccag cagctcaaac 1381 agatagctgc taatcgtcag cagcatgccc ggatgcagca gcaccagcag cagcaccagc 1441 ctaccaactg gtcagccttg ccctcctctg ctggaccatc accaggtcca tttgggcagg 1501 agaaaatccc cagcccttct tttggtcagc agacattcag cccacagagc tcccccatgc 1561 ctggggtagc tggcggcagc ggccagtcga aagtaatggc taactacatg tacaaggccg 1621 gcccctcagc ccagggtggg cacctagatg tcctcatgca gcaaaagcct caggatctca 1681 gtcgaagttt tattaacaac ccgcacccag ccatggagcc ccgtcagggc aacaccaagc 1741 ctttgtttca ttttaactca gatcaagcga accagcagat gccttctgtt ttgccttccc 1801 agaacaagcc ttctctccta cactacaccc aacagcaaca gcagcaacag cagcagcagc 1861 agcagcagca gcagcagcaa cagcagcagc agcagcaaca gcaacagcaa cagcaacagc 1921 agagttcaat ttcagctcaa caacagcaac agcagcagag ctcaatttca gcccaacagc 1981 agcagcagca gcaacaacag cagcagcagc agcaacaaca acagcaacaa cagcagcagc 2041 agcagcagca acaaccatct tctcagcctg cccaatctct accaagccag cctttgctaa 2101 ggtcaccttt gccacttcag caaaagctcc tacttcagca aatgcagaat cagcccattg 2161 caggaatggg ataccaagtc tcccaacaac agagacagga tcaacactct gtggtaggcc 2221 agaacacagg ccccagtcca agtcctaacc cctgctcaaa tccaaacact ggaagtggtt 2281 acatgaactc ccagcaatca ctgttgaatc agcaattgat gggaaagaag cagactctac 2341 agaggcagat catggagcag aaacagcaac ttcttctcca gcagcagatg ctggctgacg 2401 cggagaaaat tgctccacaa gatcagataa accgacattt gtcaaggcca cctccagatt 2461 ataaagacca aagaagaaat gtgggcaata tgcaaccaac tgctcagtat tctggtggct 2521 catccacaat aagcttaaac tctaaccagg ctttggcaaa cccagtttca acacacacca 2581 ttttaactcc caattccagc ctcctgtcta cttctcacgg gacaagaatg ccatcattat 2641 ctacagcagt tcagaatatg gggatgtatg gaaatctgcc ttgtaatcaa cctaacacat 2701 acagtgtcac ttcaggaatg aatcaattga cccaacagag aaacccaaag caattgttag 2761 caaatcaaaa caaccctatg atgccacggc cacctacctt agggccaagt aataataaca 2821 atgtagccac ttttggagct ggatctgttg gtaattcaca acaattgaga ccaaatttaa 2881 cccatagtat ggcaagcatg ccaccacaga gaacatcaaa cgtaatgatc acatccaaca 2941 caactgcacc aaactgggcc tctcaagaag gaacaagcaa acagcaagaa gccctgacgt 3001 ctgcaggagt ccgcttcccc acaggtacac ctgcagccta taccccaaat cagtcactgc 3061 aacaggcagt aggtagccag caattttccc agagggcagt ggctcctcct aaccagttaa 3121 caccagcagt gcaaatgaga cccatgaacc aaatgagcca aacactaaat gggcaaacca 3181 tgggtcccct caggggtctg aatctcagac ccaatcagct aagcacacag attttgccta 3241 atttgaatca gtcaggaaca gggttgaatc agtcgaggac gggcatcaac cagccaccat 3301 ccctgacgcc cagcaatttt ccttcaccca accaaagttc cagggctttt caaggaactg 3361 accacagcag tgacttagct tttgacttcc tcagccaaca aaatgataac atgggccctg 3421 ccctaaacag tgatgctgat ttcattgatt ctttattgaa gacagagcct ggtaatgatg 3481 actggatgaa agacatcaat cttgatgaaa tcttggggaa caattccttg ccaactttct 3541 tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt 3601 aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta