Construct: ORF ccsbBroad304_12896

Construct Description:

• Construct Type: ORF
• Other Identifiers: ORF001880.1_s304c1, BRDN0000409061
• Derived from: ccsbBroadEn_12896
• DNA Barcode: None
• Epitope Tag: V5
• Notes: No stop codon in insert

Originally Annotated References:

• Gene: DISP1 (84976)

Vector Information:

• Vector Backbone: pLX_304
• Pol II Cassette 1: PGK-BlastR
• Pol II Cassette 2: CMV-ccsbBroad304_12896
• Selection Marker: BlastR
• Visible Reporter: n/a
• Epitope Tag: V5

Current transcripts matched by this ORF:

	Taxon	Gene	Symbol	Description	Transcript	Nuc. Match %	Prot. Match %	Match Diffs
1	human	84976	DISP1	dispatched RND transporter ...	NM_001350630.2	71.5%	71.5%	1_1092del;3093A>C;3493G>A
2	human	84976	DISP1	dispatched RND transporter ...	XM_006711594.3	68.8%	68.8%	1_1239del;3240A>C;3640G>A
3	human	84976	DISP1	dispatched RND transporter ...	XM_017002612.1	68.8%	68.8%	1_1239del;3240A>C;3640G>A
4	human	84976	DISP1	dispatched RND transporter ...	NM_001369594.1	60.1%	60.1%	1_1821del;3822A>C;4222G>A
5	human	84976	DISP1	dispatched RND transporter ...	NM_032890.5	60.1%	60.1%	1_1821del;3822A>C;4222G>A
6	human	84976	DISP1	dispatched RND transporter ...	XM_006711592.2	60.1%	60.1%	1_1821del;3822A>C;4222G>A
7	human	84976	DISP1	dispatched RND transporter ...	XM_011510072.2	60.1%	60.1%	1_1821del;3822A>C;4222G>A
8	human	84976	DISP1	dispatched RND transporter ...	XM_011510073.2	60.1%	60.1%	1_1821del;3822A>C;4222G>A
9	human	84976	DISP1	dispatched RND transporter ...	XM_011510074.2	60.1%	60.1%	1_1821del;3822A>C;4222G>A
10	human	84976	DISP1	dispatched RND transporter ...	XM_011510075.2	60.1%	60.1%	1_1821del;3822A>C;4222G>A
11	human	84976	DISP1	dispatched RND transporter ...	XM_011510077.2	60.1%	60.1%	1_1821del;3822A>C;4222G>A
12	human	84976	DISP1	dispatched RND transporter ...	XM_017002611.1	60.1%	60.1%	1_1821del;3822A>C;4222G>A

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:

66

ORF end:

2817

ORF length:

2751

Sequence:

1	ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61	ttggcatgtt cgtcaccagt tttaccactg ctgctgcctt ttatgctaac tatgttagca
121	acattacagc aatccgatgc tttggggttt atgcggggac agctatattg gtgaattacg
181	ttttgatggt cacatggctt ccagcagttg ttgtgctgca tgagcggtat cttcttaata
241	tattcacttg cttcaaaaag ccccagcagc aaatatatga taacaaaagc tgctggacag
301	tggcttgcca gaagtgccac aaagtactct ttgccatttc agaagcatct cgaatttttt
361	tcgaaaaagt attgccatgc attgtcatta agtttcgcta cctttggctg ttttggttcc
421	ttgccttaac tgtaggtggg gcctacattg tatgtataaa tccaaagatg aaactgccct
481	cactggagtt atccgagttc caggtgttcc ggtcgtccca tccttttgag cgttatgatg
541	ctgaatacaa aaagcttttc atgtttgaac gtgttcacca tggcgaggag ctccacatgc
601	ccatcacagt aatctggggc gtgtccccag aagacaatgg caacccacta aatcccaaga
661	gtaaagggaa gttgacatta gatagcagtt ttaacatcgc cagcccagct tcccaggcct
721	ggattttgca cttctgtcaa aaactgagaa accaaacatt cttttaccag actgatgaac
781	aggacttcac cagctgcttc attgagacat tcaaacagtg gatggaaaac caggactgtg
841	atgagcctgc cctgtaccca tgctgcagcc actggagctt cccctacaag caagagattt
901	ttgaactgtg catcaagaga gctatcatgg agctggaaag gagtacaggg taccatttgg
961	atagcaaaac cccagggccg aggtttgata tcaatgatac tatcagggca gtggtgttag
1021	agttccagag tacctacctc ttcacactgg cttatgaaaa gatgcatcag ttttataaag
1081	aggtggactc gtggatatcc agtgagctga gttcggcccc tgaaggcctc agcaatggtt
1141	ggtttgtcag caatctggag ttctatgacc tccaggatag cctctccgat ggcaccctca
1201	ttgccatggg gctgtcagtt gctgttgcat ttagcgtgat gctgctgaca acttggaaca
1261	tcatcataag cctttatgcc atcatttcaa ttgctggaac gatatttgtc actgttggtt
1321	ctcttgtcct gctgggctgg gagctcaatg tgttggaatc tgtcaccatt tcggttgccg
1381	tcggcttgtc tgtagacttt gccgtccatt atggggttgc ctaccgcttg gctccagatc
1441	ccgaccgaga aggcaaagtg atcttctctc tgagtcgcgt gggctctgcg atggccatgg
1501	ctgccctgac caccttcgtg gcaggggcca tgatgatgcc ctccacagtt ctagcttaca
1561	cccagctggg caccttcatg atgctcatca tgtgtatcag ttgggctttc gccaccttct
1621	ttttccagtg catgtgccgg tgccttggac cacagggtac ctgtggtcag attcctttac
1681	ctaaaaaact acagtgcagt gccttttccc atgccttgtc tacaagtccc agtgacaagg
1741	gacaaagcaa aacacatacc ataaatgctt atcatttaga tcccaggggc ccaaaatctg
1801	aactggagca tgagttttat gaattagaac ctctggcttc ccacagctgc actgcccctg
1861	agaagaccac ttatgaagag acccacatct gctctgaatt tttcaacagc caagcaaaga
1921	atttagggat gcctgtgcat gcagcttaca acagtgaact cagcaaaagc actgaaagtg
1981	acgctggctc tgccttgtta cagccccctc ttgaacagca taccgtgtgt cacttcttct
2041	ctctgaatca gagatgtagc tgccccgatg cctacaaaca cttgaactat ggcccacact
2101	cttgccagca gatgggggac tgcttgtgcc accagtgctc tcctaccact agcagctttg
2161	tccagatcca aaacggcgtg gcacctctga aggccacaca ccaagctgtc gagggctttg
2221	tgcaccccat cacgcacatc caccactgtc cctgcctgca gggcagagta aagccagccg
2281	gaatgcagaa ttctctgcct aggaattttt tcctccaccc agtgcagcac attcaggccc
2341	aagaaaaaat tggcaagacc aatgtacaca gtcttcagag gagcatagaa gagcatcttc
2401	caaagatggc agagccatcg tcatttgtct gcagaagcac tggatcgtta ctcaaaacgt
2461	gttgcaaccc cgagaataaa caaagggaac tctgtaaaaa tagagacgtg agcaatctgg
2521	agagcagtgg agggactgaa aacaaggcag gagggaaagt ggagctgagc ttgtcacaga
2581	cggatgcaag tgtgaactca gaacatttca atcagaatga accaaaagtc ctatttaatc
2641	atttaatggg ggaggctggt tgtaggtctt gcccaaataa ttcacaaagt tgtggcagaa
2701	ttgtgagagt gaagtgcaat tctgtggact gtcaaatgcc aaacatggaa gccaatgtgc
2761	ctgctgtatt aacacactcg gaactttctg gtgaaagttt gttaataaaa acactatacc
2821	caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt
2881	ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga
2941	tta

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