Construct: ORF ccsbBroad304_12910

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010638.1_s304c1, BRDN0000406891
Derived from:
ccsbBroadEn_12910
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SYDE1 (85360)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_12910
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 85360 SYDE1 synapse defective Rho GTPas... NM_001300910.2 100% 100%
2 human 85360 SYDE1 synapse defective Rho GTPas... NM_033025.6 90.8% 90.8% 88_288del
3 human 85360 SYDE1 synapse defective Rho GTPas... XM_024451750.1 76.1% 59.8% 88_288del;1416_1417ins161;1881_1882ins163
4 mouse 71709 Syde1 synapse defective 1, Rho GT... NM_027875.1 74.8% 79.9% (many diffs)
5 mouse 71709 Syde1 synapse defective 1, Rho GT... XM_006514148.1 53.1% 55.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2070
ORF length:
2004
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc cgagccgcta ctcaggaaaa ccttctcccg cctgcggggc cgggagaaac
121ttccccggaa aaagtcggac gccaaggagc gcgggcctgg ggtacctggc actggggagc
181ccgccggcga gatctggtac aaccccatcc ctgaggaaga ccccagacct ccagcacctg
241agcccccggg gccacagcct ggctcagctg agtcagaggg cctggccccc caaggtgcag
301cccccgccag ccccccaacc aaagcctccc gcaccaagtc cccgggcccc gccaggcgcc
361tctccataaa gatgaagaag ctgccggaac tgcggcgccg cctgagcctg cgaggccccc
421gggctggcag ggagcgcgag agggctgccc ctgcgggctc cgtcatcagc cgctaccacc
481tggacagcag cgtggggggc cccgggccgg cagcagggcc tgggggcacc cggagcccga
541gggccggtta cctcagcgac ggggactcac cggagcgccc agctgggccc ccatcaccca
601cctccttccg gccctacgag gtgggtcccg cagcccgggc acccccggcc gcactctggg
661gccgcctcag cctgcacctg tacggtctcg gggggctgcg gccagcgccg ggggccaccc
721ccagggacct ctgctgccta ctgcaagtgg atggggaggc cagggcccga acagggccac
781tgcgaggggg gccggacttc ctgcggctgg accacacctt ccacctggag ctggaggccg
841ccaggctcct gcgcgccctg gtgcttgcgt gggaccctgg cgtgagaagg caccggccct
901gtgcccaggg caccgtgctg ctgcccacgg tcttccgagg gtgccaggcc caacagctgg
961ccgtgcgcct ggagcctcag gggctgctgt atgccaagct gaccctgtcg gagcagcagg
1021aagcccctgc cacagctgag ccccgcgtct ttgggctgcc cctgccactg ctggtggagc
1081gggagcggcc ccccggccag gtgcccctca tcatccagaa gtgcgttggg cagatcgagc
1141gccgagggct gcgggtagtg ggactgtacc gtctttgtgg ctcagcggca gtgaagaaag
1201agcttcggga tgcctttgag cgggacagtg cagcggtctg cctatctgag gacctgtacc
1261ccgatatcaa tgtcatcact ggcatcctca aggattatct tcgagagttg cccaccccac
1321tcatcaccca gcccctgtat aaggtggtac tggaggccat ggcccgggac cccccaaaca
1381gagttccccc caccactgag ggcacccgag ggctcctcag ctgcctgcca gatgtggaaa
1441gggccacgct gacgcttctc ctggaccacc tgcgcctcgt ctcctccttc catgcctaca
1501accgcatgac cccacagaac ttggccgtgt gcttcgggcc tgtgctgctg ccggcacgcc
1561aggcgcccac aaggcctcgt gcccgcagct ccggcccagg ccttgccagt gcagtggact
1621tcaagcacca catcgaggtg ctgcactacc tgctgcagtc ttggccagat ccccgcctgc
1681cccgacaatc tccagatgtc gcgccttact tgcgacccaa acgacagcca cctctgcacc
1741tgccgctggc agaccccgaa gtggtgactc ggccccgcgg tcgaggaggc cccgaaagcc
1801ccccgagcaa ccgctacgcc ggcgactgga gcgtttgcgg gcgggacttc ctgccctgtg
1861ggcgggattt cctgtccggg ccagactacg accacgtgac gggcagtgac agcgaggacg
1921aggacgagga ggtcggcgag ccgagggtca ccggtgactt cgaagacgac ttcgatgcgc
1981ccttcaaccc gcacctgaat ctcaaagact tcgacgccct catcctggat ctggagagag
2041agctctccaa gcaaatcaac gtgtgcctct gcccaacttt cttgtacaaa gtggttggta
2101agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc
2161ggtggcgcgt taagtcgaca atcaacctct ggatta

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