Construct: ORF ccsbBroad304_12970
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008354.1_s304c1, BRDN0000402657
- Derived from:
- ccsbBroadEn_12970
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CALML4 (91860)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12970
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 91860 | CALML4 | calmodulin like 4 | NM_001286694.1 | 100% | 100% | |
| 2 | human | 91860 | CALML4 | calmodulin like 4 | NM_001031733.2 | 72.7% | 66.4% | (many diffs) |
| 3 | human | 91860 | CALML4 | calmodulin like 4 | NM_001286695.1 | 67.5% | 67.5% | 0_1ins117 |
| 4 | human | 91860 | CALML4 | calmodulin like 4 | NM_033429.2 | 61.2% | 61.2% | 1_228del |
| 5 | mouse | 75600 | Calml4 | calmodulin-like 4 | XM_006511530.1 | 83% | 85.8% | (many diffs) |
| 6 | mouse | 75600 | Calml4 | calmodulin-like 4 | NM_138304.2 | 66.6% | 67.9% | (many diffs) |
| 7 | mouse | 75600 | Calml4 | calmodulin-like 4 | XM_006511529.3 | 44.2% | 45.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 426
- ORF length:
- 360
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggt ggccatgagg tgcctggggg ccagcccgac gccaggggag gtgcagcggc 121 acctgcagac ccacgggata gacggaaatg gagagctgga tttctccact tttctgacca 181 ttatgcacat gcaaataaaa caagaagacc caaagaaaga aattcttcta gccatgttga 241 tggtggacaa ggagaagaaa ggttacgtca tggcgtccga cctgcggtca aaactcacga 301 gtctggggga gaagctcacc cacaaggaag tggatgatct cttcagggaa gcagatatcg 361 aacccaatgg caaagtgaag tatgatgaat ttatccacaa gatcaccctt cctggacggg 421 actattgccc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg 481 gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca 541 acctctggat ta