Construct: ORF ccsbBroad304_13010
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001128.1_s304c1, BRDN0000405822
- Derived from:
- ccsbBroadEn_13010
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TGS1 (96764)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13010
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 96764 | TGS1 | trimethylguanosine synthase 1 | NM_001363184.2 | 18.5% | 18.5% | 1_1857del |
| 2 | human | 96764 | TGS1 | trimethylguanosine synthase 1 | NM_024831.8 | 16.5% | 16.5% | 1_2136del |
| 3 | mouse | 116940 | Tgs1 | trimethylguanosine synthase 1 | XM_006537558.2 | 16.7% | 17.5% | (many diffs) |
| 4 | mouse | 116940 | Tgs1 | trimethylguanosine synthase 1 | NM_054089.4 | 15% | 15.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 489
- ORF length:
- 423
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgag agtgattgcc attgatatcg atcctgttaa gattgccctt gctcgcaata 121 atgcagaagt ttatgggata gcagataaga tagagttcat ctgtggagat ttcttgctgc 181 tggcttcttt tttaaaggct gatgttgtgt tcctcagccc accttgggga gggccagact 241 atgccactgc agagaccttt gacattagaa caatgatgtc tcctgatggc tttgaaattt 301 tcagactttc taagaagatc actaataata ttgtttattt tcttccaaga aatgctgata 361 ttgaccaggt ggcatcctta gctgggcctg gagggcaagt ggaaatagaa cagaacttcc 421 ttaacaacaa attgaagaca atcactgcat attttggtga cctaattcga agaccagcct 481 ctgaaaccta cccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc 541 tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa 601 tcaacctctg gatta