Construct: ORF ccsbBroad304_13038
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014043.1_s304c1, BRDN0000399283
- Derived from:
- ccsbBroadEn_13038
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FMNL2 (114793)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13038
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 114793 | FMNL2 | formin like 2 | XM_011510536.3 | 18.7% | 17.6% | (many diffs) |
| 2 | human | 114793 | FMNL2 | formin like 2 | XM_005246263.2 | 16.3% | 16.3% | 1_2727del |
| 3 | human | 114793 | FMNL2 | formin like 2 | XM_011510531.1 | 16.2% | 16.2% | 1_2748del |
| 4 | human | 114793 | FMNL2 | formin like 2 | XM_005246265.3 | 15.5% | 15.1% | (many diffs) |
| 5 | human | 114793 | FMNL2 | formin like 2 | XM_011510533.3 | 15.4% | 15% | (many diffs) |
| 6 | human | 114793 | FMNL2 | formin like 2 | XM_011510535.2 | 15.4% | 14.5% | (many diffs) |
| 7 | human | 114793 | FMNL2 | formin like 2 | XM_011510534.1 | 15% | 14.1% | (many diffs) |
| 8 | human | 114793 | FMNL2 | formin like 2 | XM_011510532.3 | 14.9% | 13.5% | (many diffs) |
| 9 | human | 114793 | FMNL2 | formin like 2 | NM_052905.4 | 14.8% | 13.9% | (many diffs) |
| 10 | human | 114793 | FMNL2 | formin like 2 | XM_011510530.1 | 14.7% | 13.8% | (many diffs) |
| 11 | mouse | 71409 | Fmnl2 | formin-like 2 | XM_006498331.2 | 15% | 15.9% | (many diffs) |
| 12 | mouse | 71409 | Fmnl2 | formin-like 2 | NM_172409.2 | 14.3% | 14.9% | (many diffs) |
| 13 | mouse | 71409 | Fmnl2 | formin-like 2 | XM_006498334.2 | 14.2% | 14.8% | (many diffs) |
| 14 | mouse | 71409 | Fmnl2 | formin-like 2 | XM_006498338.2 | 14.1% | 14.2% | (many diffs) |
| 15 | mouse | 71409 | Fmnl2 | formin-like 2 | XM_006498336.2 | 14.1% | 14.1% | (many diffs) |
| 16 | mouse | 71409 | Fmnl2 | formin-like 2 | XM_006498335.3 | 13.7% | 13.3% | (many diffs) |
| 17 | mouse | 71409 | Fmnl2 | formin-like 2 | XM_006498332.2 | 13.6% | 13.2% | (many diffs) |
| 18 | mouse | 71409 | Fmnl2 | formin-like 2 | XM_017319260.1 | 13.6% | 13.2% | (many diffs) |
| 19 | mouse | 71409 | Fmnl2 | formin-like 2 | XM_006498333.3 | 13.5% | 13.5% | (many diffs) |
| 20 | mouse | 71409 | Fmnl2 | formin-like 2 | XM_006498330.2 | 13.4% | 13.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 600
- ORF length:
- 534
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga cttgaccaag agagagtaca ccatgcatga ccataacacg ctgctgaagg 121 agttcatcct caacaatgag gggaagctga agaagctgca ggatgatgcc aagatcgcac 181 aggatgcctt tgatgatgtt gtgaagtatt ttggagaaaa ccccaagaca acaccaccct 241 ctgtcttctt tcctgtcttt gtccggtttg tgaaagcata taagcaagca gaagaggaaa 301 atgagctgag gaaaaagcag gaacaagctc tcatggaaaa actcctagag caagaagctc 361 tgatggagca gcaggatcca aagtctcctt ctcataaatc aaagaggcag cagcaagagt 421 taattgcaga attaagaaga cgacaagtta aagataacag acatgtatat gagggaaaag 481 atggtgccat tgaagatatt atcacagcct taaagaagaa taatatcact aaatttccaa 541 atgttcactc gagggtaagg atttcttcta gcacaccggt ggtggaggat acacagagct 601 gcccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg 661 attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct 721 ggatta