Construct: ORF ccsbBroad304_13070
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014757.1_s304c1, BRDN0000400019
- Derived from:
- ccsbBroadEn_13070
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PIK3AP1 (118788)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13070
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 118788 | PIK3AP1 | phosphoinositide-3-kinase a... | XM_005269498.1 | 61.6% | 55.5% | (many diffs) |
| 2 | human | 118788 | PIK3AP1 | phosphoinositide-3-kinase a... | XM_005269499.1 | 61.6% | 55.5% | (many diffs) |
| 3 | human | 118788 | PIK3AP1 | phosphoinositide-3-kinase a... | NM_152309.3 | 48% | 43.5% | (many diffs) |
| 4 | human | 118788 | PIK3AP1 | phosphoinositide-3-kinase a... | XM_011539249.1 | 41.6% | 37.1% | (many diffs) |
| 5 | human | 118788 | PIK3AP1 | phosphoinositide-3-kinase a... | XM_011539248.1 | 41.5% | 36.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1278
- ORF length:
- 1212
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgag gtttttcacc agcgtggcct gctatggatc ttgtctcttt gcatctgagt 121 tgctgatcag atgcaaggac tggctcaaag gaagaccagc tctcttcact gccctgctag 181 cttgtgtcct ttatctttgt gagtggactg gtgccaagca tgtcccaggg tcatcttatg 241 ttgaaatgct tcaggccagt acatctaacc caatccctgg agatggtttc tctcgggcca 301 ctaaggactc tatgatccgc aagtttttag aaggcaacag catgggaatg accaatctgg 361 agagagatca gtgccatctt ggtcaggaag aagatgttta tcacacggtg gatgacgatg 421 aggccttttc tgtggacttg gccagcaggc cccctgtccc agtgcccaga ccagagacca 481 ctgctcctgg tgctcaccag ctgcctgaca acgaaccata catttttaaa gtttttgcag 541 aaaaaagtca agagcggcct gggaatttct acgtttcctc agagagcatc aggaaagggc 601 cgcccgtcag accatggagg gacaggcccc agtcgagtat atatgaccct tttgcgggaa 661 tgaaaacgcc aggccagcgg cagcttatca ccctccagga gcaggtgaag ctgggcattg 721 tcaacgtgga tgaggctgtg ctccacttca aagagtggca gctcaaccag aagaaacgat 781 cggagtcctt tcgtttccag caggaaaatc ttaaacggct aagagacagc atcacccgaa 841 gacagagaga gaagcaaaaa tcaggaaagc agacagactt ggagatcacg gtcccaattc 901 ggcactcaca gcacctgcct gcaaaagtgg agtttggagt ctatgagagt ggccccagga 961 aaagcgtcat tccccctagg acggagctga gacgaggaga ctggaaaaca gacagcacct 1021 ccagcacagc aagtagcaca agtaaccgct ccagcacccg gagcctcctc agtgtgagca 1081 gcgggatgga aggggacaac gaggataatg aagtccctga ggttaccaga agtcgcagtc 1141 caggcccccc acaagtggat gggacaccca ccatgtccct cgagagaccc cccagggtgc 1201 ctccgagagc tgcctcacag aggcctccga ccagggagac cttccatcct cctccacctg 1261 ttccacccag aggacgctgc ccaactttct tgtacaaagt ggttggtaag cctatcccta 1321 accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta 1381 agtcgacaat caacctctgg atta