Construct: ORF ccsbBroad304_13073
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006783.1_s304c1, BRDN0000402624
- Derived from:
- ccsbBroadEn_13073
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BORCS7 (119032)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13073
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 119032 | BORCS7 | BLOC-1 related complex subu... | NM_001136200.2 | 99% | 99% | 1_3delATG |
| 2 | human | 119032 | BORCS7 | BLOC-1 related complex subu... | NM_144591.4 | 99% | 99% | 1_3delATG |
| 3 | human | 100528007 | BORCS7-ASMT | BORCS7-ASMT readthrough (NM... | NR_037644.1 | 11.5% | 1_80del;396_2732del | |
| 4 | mouse | 66439 | Borcs7 | BLOC-1 related complex subu... | NM_025563.3 | 90.1% | 96.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 381
- ORF length:
- 315
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc gactggaacg ccagagtctc aagcgcggtt cggtcagtcc gtgaaggggc 121 ttctcacgga gaaggtgacc acctgtggta ctgacgtaat cgcgctcacc aagcaggtgc 181 tgaaaggctc ccggagctcc gagctgctag gtcaggcagc tcgaaacatg gtactccagg 241 aagatgccat cttgcactca gaagatagtt taaggaagat ggcaataata acaacacatc 301 ttcaatacca gcaagaagct attcagaaga atgttgaaca gtcatcggat ctacaggacc 361 agttgaatca tctgttgaaa tacccaactt tcttgtacaa agtggttggt aagcctatcc 421 ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg 481 ttaagtcgac aatcaacctc tggatta