Construct: ORF ccsbBroad304_13081

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016938.1_s304c1, BRDN0000408622
Derived from:
ccsbBroadEn_13081
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TPH2 (121278)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_13081
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 121278 TPH2 tryptophan hydroxylase 2 NM_173353.4 98.6% 98.7% 105_106insGCTTCGACATTCCTGAAG;936A>G;1125A>T
2 mouse 216343 Tph2 tryptophan hydroxylase 2 NM_173391.3 86.7% 92.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1557
ORF length:
1488
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gcagccagca atgatgatgt tttccagtaa atactgggca cggagagggt
121tttccctgga ttcagcagtg cccgaagagc atcagctact tggcagctca acagcttcga
181cattcctgaa gctaaataaa cctaactctg gcaaaaatga cgacaaaggc aacaagggaa
241gcagcaaacg tgaagctgct accgaaagtg gcaagacagc agttgttttc tccttgaaga
301atgaagttgg tggattggta aaagcactga ggctctttca ggaaaaacgt gtcaacatgg
361ttcatattga atccaggaaa tctcggcgaa gaagttctga ggttgaaatc tttgtggact
421gtgagtgtgg gaaaacagaa ttcaatgagc tcattcagtt gctgaaattt caaaccacta
481ttgtgacgct gaatcctcca gagaacattt ggacagagga agaagagcta gaggatgtgc
541cctggttccc tcggaagatc tctgagttag acaaatgctc tcacagagtt ctcatgtatg
601gttctgagct tgatgctgac cacccaggat ttaaggacaa tgtctatcga cagagaagaa
661agtattttgt ggatgtggcc atgggttata aatatggtca gcccattccc agggtggagt
721atactgaaga agaaactaaa acttggggtg ttgtattccg ggagctctcc aaactctatc
781ccactcatgc ttgccgagag tatttgaaaa acttccctct gctgactaaa tactgtggct
841acagagagga caatgtgcct caactcgaag atgtctccat gtttctgaaa gaaaggtctg
901gcttcacggt gaggccggtg gctggatacc tgagcccacg agactttctg gcaggactgg
961cctacagagt gttccactgt acccagtaca tccggcatgg ctcagatccc ctctacaccc
1021cggaaccaga cacatgccat gaactcttgg gacatgttcc actacttgcg gatcctaagt
1081ttgctcagtt ttcacaagaa ataggtctgg cgtctctggg agcatcagat gaagatgttc
1141agaaactagc cacgtgctat ttcttcacaa tcgagtttgg cctttgcaag caagaagggc
1201aactgcgggc ttatggagca ggactccttt cctccattgg agaattaaag cacgcccttt
1261ctgacaaggc atgtgtgaaa gcctttgacc caaagacaac ttgcttacag gaatgcctta
1321tcaccacctt ccaggaagcc tactttgttt cagaaagttt tgaagaagcc aaagaaaaga
1381tgagggactt tgcaaagtca attacccgtc ccttctcagt atacttcaat ccctacacac
1441agagtattga aattctgaaa gacaccagaa gtattgaaaa tgtggtgcag gaccttcgca
1501gcgacttgaa tacagtgtgt gatgctttaa acaaaatgaa ccaatatctg gggattttgc
1561caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt
1621ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga
1681tta

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