Construct: ORF ccsbBroad304_13088
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003990.1_s304c1, BRDN0000409628
- Derived from:
- ccsbBroadEn_13088
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AK7 (122481)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13088
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 122481 | AK7 | adenylate kinase 7 | NM_152327.5 | 90.5% | 90.4% | (many diffs) |
| 2 | human | 122481 | AK7 | adenylate kinase 7 | XM_006720021.2 | 89.6% | 89.5% | (many diffs) |
| 3 | human | 122481 | AK7 | adenylate kinase 7 | NM_001350890.2 | 87.3% | 87.2% | (many diffs) |
| 4 | human | 122481 | AK7 | adenylate kinase 7 | NM_001350891.2 | 86.9% | 86.8% | (many diffs) |
| 5 | human | 122481 | AK7 | adenylate kinase 7 | NM_001350888.2 | 86.5% | 86.4% | (many diffs) |
| 6 | human | 122481 | AK7 | adenylate kinase 7 | XM_006720022.2 | 86.1% | 86% | (many diffs) |
| 7 | human | 122481 | AK7 | adenylate kinase 7 | NM_001350892.2 | 81.4% | 81.3% | (many diffs) |
| 8 | human | 122481 | AK7 | adenylate kinase 7 | XM_017020958.1 | 80.6% | 80.5% | (many diffs) |
| 9 | human | 122481 | AK7 | adenylate kinase 7 | XM_017020960.1 | 77% | 76.9% | (many diffs) |
| 10 | mouse | 78801 | Ak7 | adenylate kinase 7 | XM_006516391.3 | 57.4% | 59.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2034
- ORF length:
- 1968
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc tgaagaagag gaaactgctg ctctcacgga gaaggttatc cggacccaga 121 gggtgtttat aaacctgttg gattcctaca gcagcggaaa catcgggaag tttctatcta 181 actgtgtagt tggggcttcg cttgaagaaa ttacagagga agaggaagag gaagatgaaa 241 ataagtcagc tatgctggaa gcttcctcaa ccaaggtgaa ggaaggcaca ttccagattg 301 tgggcacgct gtccaagcct gacagcccgc ggcctgactt tgcggtggag acgtactctg 361 ccatctctcg agaagacctt ctcatgcgcc tgctggagtg tgatgttatt atttataaca 421 tcactgagag ctcacagcaa atggaggaag ccatctgggc agtctctgca ctcagtgaag 481 aagtcagcca ctttgaaaag cgaaagctat ttattttact gtcgacggtg atgacttggg 541 cgcgctccaa agccctggac cccgaggatt ctgaggttcc attcactgaa gaagattatc 601 gaagaagaaa gtctcatcct aattttctgg accacataaa tgctgaaaaa atggttctca 661 aatttggaaa aaaggccaga aaatttgcag catacgtagt tgctgctgga ctccagtatg 721 gagcggaagg aggcatgtta cacacatttt ttaagatggc ttggttgggc gagattcctg 781 cattaccagt ttttggcgat ggaacaaatg taattccaac aatccatgtt cttgatctag 841 caggagtgat acaaaacgtc atagatcacg tgccaaagcc tcactacctg gttgctgtgg 901 atgagtctgt tcataccctg gaagacatag tcaagtgtat cagtaaaaat actggccctg 961 ggaaaatcca gaaaataccc agagaaaatg catacctaac caaggactta acgcaagatt 1021 gtcttgacca tttactggtc aacttgagaa tggaagcgct ctttgtgaag gagaatttta 1081 atattcgatg ggctgcccaa acaggatttg tggaaaatat caacactatc ctcaaggagt 1141 acaagcaaag cagaggattg atgccaatca agatctgcat tcttggtccc cctgctgtgg 1201 gaaaatccag tattgctaaa gaattggcca agtactacaa actgcatcac atccaactga 1261 aggatgtcat ttctgaagcc atagcaaaac tggaggcgat tgttgcccct aacgatgtag 1321 gggaaggaga agaagaagtc gaagaggaag aggaggagga gaatgtggaa gatgcacagg 1381 agctcctaga tggcatcaag gagagcatgg agcagaatgc aggtcaacta gacgatcaat 1441 atataattag atttatgaaa gaaaagctaa aatcaatgcc ttgcaggaat caaggttata 1501 ttttggatgg attcccaaag acctatgatc aagcaaaaga cctgttcaat caggaagatg 1561 aggaggagga agatgatgtc agaggcagaa tgtttccctt tgataaatta attatacctg 1621 aattcgtttg tgcactggat gcttcggatg agtttctgaa ggagcgtgtg ataaaccttc 1681 ctgagagcat cgtggcgggg acccactaca gccaagaccg attcctccgg gctctgagca 1741 actaccggga catcaatatc gacgatgaga ctgtcttcaa ctattttgat gaacttgaaa 1801 ttcacccgat acatattgat gtaggaaaac ttgaagatgc tcagaataga cttgctatca 1861 aacagctcat caaagagact ggggagcctc gaaattatgg tttaacagac gaagaaaagg 1921 cagaagagga gcggaaggct gcggaggagc ggctggccag ggaggctgct gaggaagcag 1981 aacgcgagca ccaggaggcc gtggagatgg cagagaagat agctcgctgg gagtacccaa 2041 ctttcttgta caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta 2101 cgtagtaatg agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta