Construct: ORF ccsbBroad304_13099

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011273.1_s304c1, BRDN0000461177
Derived from:
ccsbBroadEn_13099
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TMEM266 (123591)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_13099
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 123591 TMEM266 transmembrane protein 266 XM_005254160.3 99.8% 99.4% 620G>A;728C>T
2 human 123591 TMEM266 transmembrane protein 266 XM_017021916.1 99.8% 99.4% 620G>A;728C>T
3 human 123591 TMEM266 transmembrane protein 266 XM_017021917.1 99.8% 99.4% 620G>A;728C>T
4 human 123591 TMEM266 transmembrane protein 266 NM_152335.3 65.2% 64.9% 1_552del;1172G>A;1280C>T
5 human 123591 TMEM266 transmembrane protein 266 XM_017021915.1 65.2% 64.9% 1_552del;1172G>A;1280C>T
6 mouse 244886 Tmem266 transmembrane protein 266 XM_006511162.1 72% 72% (many diffs)
7 mouse 244886 Tmem266 transmembrane protein 266 XM_006511163.3 72% 72% (many diffs)
8 mouse 244886 Tmem266 transmembrane protein 266 XM_006511164.1 72% 72% (many diffs)
9 mouse 244886 Tmem266 transmembrane protein 266 XM_006511159.1 62.9% 62.9% (many diffs)
10 mouse 244886 Tmem266 transmembrane protein 266 XM_006511160.3 62.9% 62.9% (many diffs)
11 mouse 244886 Tmem266 transmembrane protein 266 XM_006511161.2 62.9% 62.9% (many diffs)
12 mouse 244886 Tmem266 transmembrane protein 266 NM_172923.3 55.3% 55.3% (many diffs)
13 mouse 244886 Tmem266 transmembrane protein 266 XM_011242746.2 33.2% 32.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1110
ORF length:
1041
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat ggtggcatcc actgtggcca atggacccag gagcccctgg gacgccatca
121gcctcatcat catgctccgg atctggaggg tgaagagggt cattgatgcc tacgtcctgc
181cagtgaagct ggagatggag atggttatcc agcagtacga gaaggccaag gtcatccaag
241acgagcagct ggagaggctg acgcagatct gtcaggagca agggtttgag atccggcagc
301tgcgcgcgca cctggcgcag caggacctgg acctggctgc cgagcgcgaa gcggcgctcc
361aggccccgca cgtgctcagc cagccgcgca gccgcttcaa agtgttggag gccggcacgt
421gggacgagga gacggcggcc gagagcgtcg tggaggagct gcagccctcg caagaagcca
481cgatgaagga cgacatgaac agctacatca gtcagtatta caatgggccc agcagtgaca
541gcggtgtccc agagccagct gtgtgtatgg tcaccacggc cgcaatagac attcaccagc
601ccaacatctc ctcggacctc ttctctctgg acatgcccct caaactcggc ggtaatggca
661ccagcgccac ctcggagagt gcctcccaca gctcagtcac ccgggcccag agtgacagca
721gccagacgct gggctcctcc atggactgca gcactgcccg cgaggagccg tcctctgagc
781ccggcccttc tcccctgccg ctgccatccc agcagcaggt ggaggaggcc acagtccagg
841acctgctgtc ctccctgtcg gaggacccct gcccttccca gaaggccttg gacccagccc
901ccctcgcccg gcccagccca gcgggctcgg cccaaaccag ccccgagctg gaacacaggg
961taagtctgtt caaccagaag aaccaggagg gcttcactgt ctttcagatc aggcctgtca
1021tccacttcca gcccactgtg cccatgctgg aggacaagtt cagatctttg gaatccaaag
1081agcaaaagct gcacagggtc cctgaggcct tgccaacttt cttgtacaaa gtggttggta
1141agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc
1201ggtggcgcgt taagtcgaca atcaacctct ggatta

Download FASTA (ORF) (Full)