Construct: ORF ccsbBroad304_13108
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006367.1_s304c1, BRDN0000405951
- Derived from:
- ccsbBroadEn_13108
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KIF19 (124602)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13108
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 124602 | KIF19 | kinesin family member 19 | XR_001752425.1 | 14.2% | (many diffs) | |
| 2 | human | 124602 | KIF19 | kinesin family member 19 | XR_001752426.1 | 13.8% | (many diffs) | |
| 3 | human | 124602 | KIF19 | kinesin family member 19 | XM_017024158.1 | 13.1% | 3.9% | 1_112del;470_2721del |
| 4 | human | 124602 | KIF19 | kinesin family member 19 | XM_017024157.1 | 13.1% | 3.8% | 1_112del;470_2724del |
| 5 | human | 124602 | KIF19 | kinesin family member 19 | XM_017024156.1 | 12.5% | 3.7% | (many diffs) |
| 6 | human | 124602 | KIF19 | kinesin family member 19 | XM_017024155.1 | 12.4% | 3.7% | (many diffs) |
| 7 | human | 124602 | KIF19 | kinesin family member 19 | XM_017024154.1 | 12.4% | 3.7% | 1_112del;470_2868del |
| 8 | human | 124602 | KIF19 | kinesin family member 19 | XM_005257030.1 | 12.4% | 3.6% | 1_112del;470_2871del |
| 9 | human | 124602 | KIF19 | kinesin family member 19 | XM_017024153.1 | 12.2% | 3.6% | 1_112del;470_2907del |
| 10 | human | 124602 | KIF19 | kinesin family member 19 | XM_017024152.1 | 12.2% | 3.6% | 1_112del;470_2910del |
| 11 | human | 124602 | KIF19 | kinesin family member 19 | NM_153209.4 | 11.8% | 3.5% | (many diffs) |
| 12 | human | 124602 | KIF19 | kinesin family member 19 | XM_005257029.1 | 11.8% | 3.5% | (many diffs) |
| 13 | human | 124602 | KIF19 | kinesin family member 19 | XM_017024151.1 | 11.7% | 3.5% | (many diffs) |
| 14 | human | 124602 | KIF19 | kinesin family member 19 | XM_017024150.1 | 11.7% | 3.5% | (many diffs) |
| 15 | mouse | 286942 | Kif19a | kinesin family member 19A | NM_001102615.1 | 10.9% | 2.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 423
- ORF length:
- 357
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgag cagatggtgg ttctcatgga cccaatggag gatcccgacg acatcctgcg 121 ggcgcatcgc tcccgggaga agtcctacct gttcgacgtg gcctttgact tcaccgccac 181 ccaggagatg gtgtatcagg ccaccaccaa gagcctcatc gagggcgtca tctcaggcta 241 caatgccact gtctttgcct atggccccac aggctgtggg aaaacctaca ccatgctggg 301 cacagaccag gagcctggca tctatgttca gaccctcaac gacctcttcc gtgccatcga 361 ggagaccagc aatgacatgg agtatgaggt ctccatgtcc tacctggaga tcatgcagct 421 gctgcccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc 481 tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc 541 tctggatta