Construct: ORF ccsbBroad304_13130
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002727.1_s304c1, BRDN0000411771
- Derived from:
- ccsbBroadEn_13130
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RNF19B (127544)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13130
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 127544 | RNF19B | ring finger protein 19B | NM_153341.4 | 74.8% | 74.8% | 1_552del |
| 2 | human | 127544 | RNF19B | ring finger protein 19B | XM_006710356.2 | 74.8% | 74.8% | 1_552del |
| 3 | human | 127544 | RNF19B | ring finger protein 19B | NM_001300826.2 | 74.7% | 74.7% | 1_552del;838_839insCAG |
| 4 | human | 127544 | RNF19B | ring finger protein 19B | XM_006710357.3 | 68.8% | 68.8% | 1_552del;1610_1611ins132 |
| 5 | human | 127544 | RNF19B | ring finger protein 19B | XM_006710358.3 | 54.8% | 54.6% | (many diffs) |
| 6 | human | 127544 | RNF19B | ring finger protein 19B | NM_001127361.3 | 54.6% | 54.5% | (many diffs) |
| 7 | mouse | 75234 | Rnf19b | ring finger protein 19B | XM_006503458.3 | 68.2% | 71.6% | (many diffs) |
| 8 | mouse | 75234 | Rnf19b | ring finger protein 19B | NM_029219.1 | 68.1% | 71.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1713
- ORF length:
- 1644
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gcacaagtac gaggagttca tgctgcgccg ctacctagcc tcggaccccg 121 actgccgctg gtgcccggcc ccggactgcg gttatgctgt tattgcctat ggctgtgcca 181 gctgcccgaa gctaacttgt gagagggaag gttgccagac tgagttctgc taccactgca 241 agcagatatg gcatccaaat cagacatgcg atatggcccg tcaacagagg gcccagactt 301 tacgagttcg gaccaaacac acttcaggtc tcagttatgg gcaagaatct ggaccagcag 361 atgacatcaa gccatgccca cgatgcagtg catacattat caagatgaat gatggaagct 421 gtaatcacat gacctgtgca gtgtgtggct gtgaattctg ttggctttgt atgaaagaga 481 tctcagactt gcattacctc agcccctctg gctgtacatt ctggggcaag aagccatgga 541 gccgtaagaa gaaaattctt tggcagctgg gcacgttgat tggtgctcca gtggggattt 601 ctctcattgc tggcattgcc attcctgcca tggtcattgg cattcctgtt tatgttggaa 661 ggaagattca cagcaggtat gagggaagga aaacctccaa acacaagagg aatttggcta 721 tcactggagg agtgactttg tcggtcattg catccccagt tattgctgca gttagtgttg 781 gtattggtgt ccccattatg ctggcatatg tttatggggt tgtgcccatt tctctttgtc 841 gtggaggcgg ctgtggagtt agcacagcca acggaaaagg agtgaaaatt gaatttgatg 901 aagatgatgg tccaatcaca gtggcagatg cctggagagc cctcaagaat cccagcattg 961 gggaaagcag cattgaaggc ctgactagtg tattgagcac tagtggaagc cctacagatg 1021 gacttagtgt tatgcaaggt ccttacagcg aaacggccag ctttgcagcc ctctcagggg 1081 gcacgctgag tggcggcatt ctctccagtg gcaagggaaa atatagcagg ttagaagttc 1141 aagccgatgt ccaaaaggaa attttcccca aagacacagc cagtcttggt gcaattagtg 1201 acaacgcaag cactcgtgct atggccggtt ccataatcag ttcctacaac ccacaggaca 1261 gagaatgcaa caatatggaa atccaagtgg acattgaagc caaaccaagc cactatcagc 1321 tggtgagtgg aagcagcacg gaggactcgc tccatgttca tgctcagatg gcagagaatg 1381 aagaagaagg tagtggtggc ggaggcagtg aagaggatcc cccctgcaga caccaaagct 1441 gtgaacagaa agactgcctg gccagcaaac cttgggacat cagcctggcc cagcctgaaa 1501 gcatccgcag tgacctagag agttctgatg cacagtcaga cgatgtgcca gacatcacct 1561 cagatgagtg tggctccccc cgctcccata ctgcagcctg cccctcgacc cccagagccc 1621 aaggtgcacc gagcccaagt gcccatatga acctctctgc cctagccgag ggacaaactg 1681 tcttgaagcc agaaggtgga gaagccagag tattgccaac tttcttgtac aaagtggttg 1741 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta 1801 accggtggcg cgttaagtcg acaatcaacc tctggatta