Construct: ORF ccsbBroad304_13130

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002727.1_s304c1, BRDN0000411771
Derived from:
ccsbBroadEn_13130
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
RNF19B (127544)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_13130
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 127544 RNF19B ring finger protein 19B NM_153341.4 74.8% 74.8% 1_552del
2 human 127544 RNF19B ring finger protein 19B XM_006710356.2 74.8% 74.8% 1_552del
3 human 127544 RNF19B ring finger protein 19B NM_001300826.2 74.7% 74.7% 1_552del;838_839insCAG
4 human 127544 RNF19B ring finger protein 19B XM_006710357.3 68.8% 68.8% 1_552del;1610_1611ins132
5 human 127544 RNF19B ring finger protein 19B XM_006710358.3 54.8% 54.6% (many diffs)
6 human 127544 RNF19B ring finger protein 19B NM_001127361.3 54.6% 54.5% (many diffs)
7 mouse 75234 Rnf19b ring finger protein 19B XM_006503458.3 68.2% 71.6% (many diffs)
8 mouse 75234 Rnf19b ring finger protein 19B NM_029219.1 68.1% 71.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1713
ORF length:
1644
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gcacaagtac gaggagttca tgctgcgccg ctacctagcc tcggaccccg
121actgccgctg gtgcccggcc ccggactgcg gttatgctgt tattgcctat ggctgtgcca
181gctgcccgaa gctaacttgt gagagggaag gttgccagac tgagttctgc taccactgca
241agcagatatg gcatccaaat cagacatgcg atatggcccg tcaacagagg gcccagactt
301tacgagttcg gaccaaacac acttcaggtc tcagttatgg gcaagaatct ggaccagcag
361atgacatcaa gccatgccca cgatgcagtg catacattat caagatgaat gatggaagct
421gtaatcacat gacctgtgca gtgtgtggct gtgaattctg ttggctttgt atgaaagaga
481tctcagactt gcattacctc agcccctctg gctgtacatt ctggggcaag aagccatgga
541gccgtaagaa gaaaattctt tggcagctgg gcacgttgat tggtgctcca gtggggattt
601ctctcattgc tggcattgcc attcctgcca tggtcattgg cattcctgtt tatgttggaa
661ggaagattca cagcaggtat gagggaagga aaacctccaa acacaagagg aatttggcta
721tcactggagg agtgactttg tcggtcattg catccccagt tattgctgca gttagtgttg
781gtattggtgt ccccattatg ctggcatatg tttatggggt tgtgcccatt tctctttgtc
841gtggaggcgg ctgtggagtt agcacagcca acggaaaagg agtgaaaatt gaatttgatg
901aagatgatgg tccaatcaca gtggcagatg cctggagagc cctcaagaat cccagcattg
961gggaaagcag cattgaaggc ctgactagtg tattgagcac tagtggaagc cctacagatg
1021gacttagtgt tatgcaaggt ccttacagcg aaacggccag ctttgcagcc ctctcagggg
1081gcacgctgag tggcggcatt ctctccagtg gcaagggaaa atatagcagg ttagaagttc
1141aagccgatgt ccaaaaggaa attttcccca aagacacagc cagtcttggt gcaattagtg
1201acaacgcaag cactcgtgct atggccggtt ccataatcag ttcctacaac ccacaggaca
1261gagaatgcaa caatatggaa atccaagtgg acattgaagc caaaccaagc cactatcagc
1321tggtgagtgg aagcagcacg gaggactcgc tccatgttca tgctcagatg gcagagaatg
1381aagaagaagg tagtggtggc ggaggcagtg aagaggatcc cccctgcaga caccaaagct
1441gtgaacagaa agactgcctg gccagcaaac cttgggacat cagcctggcc cagcctgaaa
1501gcatccgcag tgacctagag agttctgatg cacagtcaga cgatgtgcca gacatcacct
1561cagatgagtg tggctccccc cgctcccata ctgcagcctg cccctcgacc cccagagccc
1621aaggtgcacc gagcccaagt gcccatatga acctctctgc cctagccgag ggacaaactg
1681tcttgaagcc agaaggtgga gaagccagag tattgccaac tttcttgtac aaagtggttg
1741gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta
1801accggtggcg cgttaagtcg acaatcaacc tctggatta

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