Construct: ORF ccsbBroad304_13136
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006447.1_s304c1, BRDN0000404373
- Derived from:
- ccsbBroadEn_13136
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EDARADD (128178)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13136
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2023 | ENO1 | enolase 1 | NM_001428.5 | 83.1% | 79.2% | (many diffs) |
| 2 | human | 2023 | ENO1 | enolase 1 | NM_001353346.3 | 80.7% | 77.4% | (many diffs) |
| 3 | human | 2023 | ENO1 | enolase 1 | NM_001201483.4 | 62.4% | 59.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1230
- ORF length:
- 1164
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtc tattctcaag atccatgcca gggagctctt tgactctcgt gggaatccca 121 ctgttgaggt tgatctcttc acctcagaag gtctcttcag agctgctgtg cccagtggtg 181 cttcaactgg tatctatgag gtcctagagc tccaggacaa tgataagact cgctatatgg 241 ggaagggtgt ctcaaagcct gttgagccca tcaataaaac tattgcacct gtcctggtta 301 gcaagaaact gaacgtcaca gaacaagaga agattgacaa acttatgata gagatggatg 361 gaacagaaaa taaatctaaa tttggtgcaa atgccattct gggagtgtcc ctcgctgcct 421 gcaaagctag tgctgttgag aagggggttc ccctgtacca ccacatcgcc gacttgtctg 481 gcaactccaa agtcatcttg ccagtcccgg tgttcaatgt catcaatggc agttctcatg 541 ctgtcaccaa gctggccatg caggagttca tggtcctccc agtcggtgca gcaaacttca 601 gggaagccat gcccattgga gcggaggttt accacagcct gaagaatgtc atcaaggaga 661 aatatgggaa agatgccacc ggtgtggggg atggaggcgc gtttgctccc aacatcctgg 721 agaataaaga aggcctggag ctgctgaaga ctgcgattgg gaaagctggc tacactgata 781 aggtgatcgt cagcatggac gtagaggcct ccgagttctt caggtctgga aagtatgacc 841 tggaattcaa gtttctcgac gaccccacca ggtacatctc acctgactgt ctggctgacc 901 tgtacaagtc cttcatcaaa aactacccag tggtgtctac tgaagatccc tttgaccagg 961 atgactgggg agcttggcag aagttcacgg ccagtgcagg aatccaggta gtggaggatg 1021 atctcagagt gaccaaccca aagaggacag cctcggccgt gaatgagaag aagtgcaact 1081 gcctcctgct caaagtgaac cagattcgct ccgtgactga gtcccttcag gcgtgcaagc 1141 tggcccaggc gaatggttgg tgtgtcatgg tgcctcatca ttctggggag actgaaaata 1201 ccttcatcac tgacctggtg gtggggctgt gcccaacttt cttgtacaaa gtggttggta 1261 agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc 1321 ggtggcgcgt taagtcgaca atcaacctct ggatta