Construct: ORF ccsbBroad304_13159

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010158.1_s304c1, BRDN0000461300
Derived from:
ccsbBroadEn_13159
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ZPLD1 (131368)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_13159
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 131368 ZPLD1 zona pellucida like domain ... NM_001329788.1 99.8% 99.7% 111A>G;725A>G
2 human 131368 ZPLD1 zona pellucida like domain ... XM_017005703.1 99.8% 99.7% 111A>G;725A>G
3 human 131368 ZPLD1 zona pellucida like domain ... XM_017005704.1 99.8% 99.7% 111A>G;725A>G
4 human 131368 ZPLD1 zona pellucida like domain ... NM_175056.2 96.1% 96% 1_48del;159A>G;773A>G
5 mouse 239852 Zpld1 zona pellucida like domain ... NM_178720.4 87.6% 91.3% (many diffs)
6 mouse 239852 Zpld1 zona pellucida like domain ... XM_006522132.2 87.6% 91.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1314
ORF length:
1245
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat ggaacaaata tggttgctgc tgcttctaac aattagagtg cttccggggt
121ctgctcagtt caacggctac aactgtgatg ccaacctcca cagtagattt cctgctgaga
181gagacatcag tgtctattgt ggagtgcagg ctattacgat gaagattaat ttttgcacgg
241tacttttctc gggttattcg gaaacagatc tggcactgaa tggaaggcat ggggactccc
301actgcagagg gttcatcaat aacaacacct ttccagcagt ggtcattttt atcatcaatc
361tcagcacctt ggagggctgt ggaaacaacc tggtggtatc cacaattcct ggagtcagtg
421cttatggaaa tgcaacttca gtgcaagtag gaaatatttc aggatatatt gatactccag
481acccaccaac aatcatcagc tatctacctg ggcttcttta caaatttagt tgtagttatc
541cattggaata cctggttaat aatacccagc ttgcttcgtc ctcagcggct atttctgtga
601gagagaacaa tggcacattt gtcagcactt tgaacctgct cctttataac gattcaacct
661acaaccagca gttaattatc cccagtatag gattaccttt gaaaaccaaa gtatttgcag
721ctgtccaagc cactaatttg gatggcagat ggaatgtttt aatggattat tgctatacta
781ccccatcagg aagcccaaat gatgacattc gatatgatct tttccttagc tgtgacaagg
841accctcagac caccgtcatt gagaatggcc gaagccagcg gggccggttt tcttttgaag
901tgttccgatt tgtgaaacac aagaatcaga aaatgtccac tgtcttcttg cactgcgtta
961ccaagctctg cagagcagat gactgcccct tccttatgcc gatttgcagc cacagagaaa
1021ggagagatgc tgggaggagg acgacttgga gcccccagag ctcttctggc agcgcggtgc
1081tctctgctgg tcccatcatt actcggagtg atgagactcc aaccaacaat tcgcaacttg
1141gttctccaag tatgcctccc ttccagctga acgccatcac cagcgcactg atatcaggaa
1201tggtcattct gggagttacg agcttttctc ttcttctgtg ctcactggcc cttctgcaca
1261ggaagggacc cacaagttta gtgttgaatg gcataagaaa cccagtcttt gacttgccaa
1321ctttcttgta caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta
1381cgtagtaatg agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta

Download FASTA (ORF) (Full)

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