Construct: ORF ccsbBroad304_13159
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010158.1_s304c1, BRDN0000461300
- Derived from:
- ccsbBroadEn_13159
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZPLD1 (131368)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13159
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 131368 | ZPLD1 | zona pellucida like domain ... | NM_001329788.1 | 99.8% | 99.7% | 111A>G;725A>G |
| 2 | human | 131368 | ZPLD1 | zona pellucida like domain ... | XM_017005703.1 | 99.8% | 99.7% | 111A>G;725A>G |
| 3 | human | 131368 | ZPLD1 | zona pellucida like domain ... | XM_017005704.1 | 99.8% | 99.7% | 111A>G;725A>G |
| 4 | human | 131368 | ZPLD1 | zona pellucida like domain ... | NM_175056.2 | 96.1% | 96% | 1_48del;159A>G;773A>G |
| 5 | mouse | 239852 | Zpld1 | zona pellucida like domain ... | NM_178720.4 | 87.6% | 91.3% | (many diffs) |
| 6 | mouse | 239852 | Zpld1 | zona pellucida like domain ... | XM_006522132.2 | 87.6% | 91.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1314
- ORF length:
- 1245
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaacaaata tggttgctgc tgcttctaac aattagagtg cttccggggt 121 ctgctcagtt caacggctac aactgtgatg ccaacctcca cagtagattt cctgctgaga 181 gagacatcag tgtctattgt ggagtgcagg ctattacgat gaagattaat ttttgcacgg 241 tacttttctc gggttattcg gaaacagatc tggcactgaa tggaaggcat ggggactccc 301 actgcagagg gttcatcaat aacaacacct ttccagcagt ggtcattttt atcatcaatc 361 tcagcacctt ggagggctgt ggaaacaacc tggtggtatc cacaattcct ggagtcagtg 421 cttatggaaa tgcaacttca gtgcaagtag gaaatatttc aggatatatt gatactccag 481 acccaccaac aatcatcagc tatctacctg ggcttcttta caaatttagt tgtagttatc 541 cattggaata cctggttaat aatacccagc ttgcttcgtc ctcagcggct atttctgtga 601 gagagaacaa tggcacattt gtcagcactt tgaacctgct cctttataac gattcaacct 661 acaaccagca gttaattatc cccagtatag gattaccttt gaaaaccaaa gtatttgcag 721 ctgtccaagc cactaatttg gatggcagat ggaatgtttt aatggattat tgctatacta 781 ccccatcagg aagcccaaat gatgacattc gatatgatct tttccttagc tgtgacaagg 841 accctcagac caccgtcatt gagaatggcc gaagccagcg gggccggttt tcttttgaag 901 tgttccgatt tgtgaaacac aagaatcaga aaatgtccac tgtcttcttg cactgcgtta 961 ccaagctctg cagagcagat gactgcccct tccttatgcc gatttgcagc cacagagaaa 1021 ggagagatgc tgggaggagg acgacttgga gcccccagag ctcttctggc agcgcggtgc 1081 tctctgctgg tcccatcatt actcggagtg atgagactcc aaccaacaat tcgcaacttg 1141 gttctccaag tatgcctccc ttccagctga acgccatcac cagcgcactg atatcaggaa 1201 tggtcattct gggagttacg agcttttctc ttcttctgtg ctcactggcc cttctgcaca 1261 ggaagggacc cacaagttta gtgttgaatg gcataagaaa cccagtcttt gacttgccaa 1321 ctttcttgta caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta 1381 cgtagtaatg agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta